Abstract:

Abstract:Objective To study the effect of trantinterol,a potent and highly selective  β2 receptor  agonist,on the cytochrome P450 enzymes (CYP450) mRNA expression and provide a theoretical basis for clinical drug combination.Methods  The cell proliferation of HepG2 cells treated with  2.4,12,60,300,1 200 and 4 800 ng/L  trantinterol was analyzed by MTT.The mRNA expression levels of CYP1A1,CYP2E1 and CYP3A5 in HepG2 cells were examined using the real-time quantitative reverse-transcriptase polymerase chain reaction in human hepatoma HepG2 cells.Results Different concentrations of trantinterol didn’[KG-*3]t inhibit the proliferation of HepG2 cells (P>0.05).Compared with control group,the mRNA expressions of CYP2E1 and CYP3A5 in trantinterol groups had no change,while the mRNA expression of CYP1A1 was inhibited (P<0.01).Conclusion Exposure of HepG2 cells to trantinterol has no effect on the mRNA expressions of CYP2E1 and CYP3A5 but inhibit the mRNA expression of CYP1A1.

Key words: trantinterol, cytochrome P450, HepG2 cell; , real-time quantitative polymerase chain reaction