Abstract:

To investigate the expression and correlation of Skp2 and P^27kip1 in colon carcinoma tissue and explore the relationship with the development of colon carcinoma.Methods  The protein expressions of Skp2,P^27kip1,P53 and Bcl-2 were detected by immunohistochemistry staining (SABC method) in 50 cases of colon carcinoma  tissue (CCT) and 20 normal colonic mucosas (NCM).The positive expression rates in different tissues were compared and the relationship between the expression and different clinicopathological parameters were analyzed.Results The expression of Skp2 in the CCT was significantly increased compared with NCM（P<0.05）.The protein expression of P^27kip1 in  CCT was significantly lower than that in NCM（P<0.05）.The expressions of Skp2 were significantly increased in the Duke’s staging C and D group,lower degree differentiation group and lymph node metastases positive group（P<0.05）.The expressions of P^27kip1 were significantly increased in the Duke’s staging A and B group,high degree differentiation group and  lymph
 node metastases negative group（P<0.05）.The expressions of P53 and Bcl-2 protein were significantly increased in the low degress differentiation CCT（P<0.05）. The negative correlation was present between  Skp2 and P^27kip1 in  CCT（r=－0.282，P<0.05）,and there were  positive correlations  between Skp2 and P53,Bcl-2  in  CCT (r=0.345，r=0.231,P<0.05）.Conclusion The abnormal expressions of both Skp2 and P^27kip1 participate in  the differentiation,infiltration and  metastases of colon carcinoma. It may be realized through  up-regulating the expressions of P53 and Bcl-2.

Key words: colon neoplasm；Skp2；P^27kip1；protein P53 ；proto-oncogene protein c-bcl-2