Abstract:

Abstract:Objective
To examine the regulatory mode of neuropeptide Y (NPY) in the central nucleus of amygdala(CeA) on feeding of fasted rats，and to explore the possible mechanism. Methods 34 male SD  rats were chosen,and 10 rats of them were divided into feeding group(n=5) and fasted 24 h group(n=5),and the rat brain was obtained. The expressions of NPY and neuropeptide Y 1 receptor(Y1R)in CeA were detected by immunohistochemical staining method. The other 24 rats were implanted cannula in CeA. The postoperative 12 rats were randomly divided into 2 groups,and the rats in one group were injected with NPY and the rats in another group were injected with saline in CeA(control 1 group),after 1,2 and 4 h,the intake of food was detected. The other postoperative 12 rats were randomly divided into 2 groups,and the rats in one group were injected with Y1R antagonist and the rats in another group were injected with saline in CeA(control 2 group),after 1,2 and 4 h,the intake of food was detected. Results  Compared with feeding group,the NPY expression in CeA of the rats in fasted 24 h group was significantly increased (P<0.05),and the Y1R expression in CeA of the rats in fasted 24 h group was significantly decreased (P<0.05). Compared with control 1 group,the intake of food of the rats   injected with NPY in CeA within 1 h was significantly increased(P<0.05);compared with control 2 group,the intake of food of the rats  injected with Y1R antagonist in CeA within 1 h was significantly decreased(P<0.05). Conclusion NPY in CeA has promotion effect on the feeding of fasted rats,and the mechanism may be regulated by Y1 receptor.
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Key words: neuropeptide Y, central nucleus of amygdala, neuropeptide Y1 receptor, feeding