Abstract:

Abstract:Objective To identify and isolate the CK17+,CD44v5+ and Bcrp1+ cells in HeLa cells，and investigate the relationship between them and  select cervical cancer stem cell markers.Methods  Flow cytometry was used to identify and isolate the CK17+,CD44v5+ and Bcrp1+ cells in HeLa cells；after isolation,the expressions of CK17 and CD44v5 in Bcrp1+ and Bcrp1-  phenotype HeLa cells were measured by  immunocytochemistry.Results HeLa cells contained 11% Bcrp1+ cells,0.7% CK17+ cells and 0.1%CD44v5+ cells.Bcrp1+ HeLa cells contained CK17+ and CD44v5+ HeLa cells,but the Bcrp1- Hela cells did not（P<0.05）.Conclusion HeLa cells expressed CK17 and CD44v5 antibody must express Bcrp1 antibody.CK17 and CD44v5 may be used as the markers of cervical cancer stem cells.

Key words: HeLa cells；carcinoma,squamous cell；CSC；CK17；CD44v5；Bcrp1；flow cytometry