Objective To study the effect of aFGF with the nuclear translocation sequenceknocked out on the mitogenic activity to NIH3T3 cells. Methods There were four groups in the experiment: waFGF, raFGF, waFGF+HS and raFGF+HS. The cell proliferation was measured with MTT and the cell cycle progression and apoptosis were measured with FCM. The expressions of bcl-2, p53 and c-fos genes were determined with RT PCR. Results As the concentrations of raFGF or waFGF were at 10-40μg•L^-1, the effect of raFGF on the cell proliferation was significantly lower than that of waFGF (P<0.001); when HS was added, the cell proliferations in waFGF+HS and raFGF+HS groups were significantly higher than those in waFGF and raFGF groups, respectively (P<0.001). The apoptotic percentages of NIH3T3 cells treated with aFGF and raFGF all decreased, but the effect of raFGF was weaker than that of waFGF. The expressions of c-fos gene in waFGF and waFGF+HS groups were higher than those in raFGF and raFGF+HS. The expressions of p53 gene in waFGF and raFGF groups increased. The expression of bcl-2 in waFGF+HS group was highest, next were those in waFGF and raFGF groups. Conclusion The mitogenic activity of raFGF is weaker than that of waFGF. They all can stimulate DNA synthesis and cell proliferation, and inhibit the NIH3T3 cell apoptosis. Heparin can enhance the aFGF activity, and act as a asistant factor to imitate the the internal environment in aFGF in vitro.

Objective To clone BMP7 gene in vitro, and determine the expression of BMP7 gene in articular chondrocytes of rabbits cultivated in vitro. Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting. Results The cloned BMP7 gene was about 1 300 bp, had the same length and sequence with those reported in GenBank. The expression of BMP7 gene in adult rabbit articular chondrocytes was determined. Conclusion The articular chondrocytes of adult rabb its transfected with pcDNA3.1-BMP7 are constructed sucessfully, and the chondr ocytes can express the target gene BMP7 stably. The transfected chondrocytes can be used in cellular transplantation therapy or in constructing the tissue engineerinig cartilage to repair the articular cartilage defect.

Objective To investigate the effect of Biondia Heriyi (BH) extract on Streptozocin(STZ)-induced diabetic rats. Methods Thirty two male rats were divided randomly into four groups: control group (A) and three groups of diabetic models. The diabetic model was established after three days according to blood glucose level (>16.65mmol•L^-1) measured by blood glucose analyzer. Then the diabetic rats were divided into three group, one was control group of diabetic rats, the other two groups were received BH (465 mg•kg^-1) and XKW (625 mg•kg^-1)into stomach respectively, the glucose concentration in blood was measured once a week. For all the three groups of diabetic rats, at the three days after injection of STZ and the end of the experiment, the insulin concentrations in blood were determined by radioimmunoassay. The morphological structure of the pancreatic island was also observed. Results After administration of BH extract and XKW for five weeks respectively ,the level of blood glucose in diabetic rats of BH-administration group decreased from (18.3±1.8) mmol•L^-1 to (5.3±1.2)mmol• L^-1, the level of blood glucose in diabetic rats of XKW-administration group decreased from (18.4±1.9)mmol•L^-1 to (5.2±0.8)mmol•L^-1,compared with the level in control group of diabetic rats ［(19.9±1.9)mmol•L^-1］,there was significant differente (P<0.001). The blood insulin level in diabetic rats of BH-administered group was increased from (18.8±2.3)mU• L^-1 to（42.2±3.5）mU•L^-1. The blood insulin level in diabetic rats of XKW-administration group increased from (18.1±3.3)mU• L^-1 to（40.9±3.7）mU•L^-1, compared with the level in the control group of diabetic rats［(18.7±3.1)mU •L^-1］, there was significant difference （P<0.01）. To corresponding the changes of the blood glucose and insulin, the morphological changes of the pancreatic island cells in diabetic rats were the changes in the cell atrophy, nucleus pyknosis, secretion particles and so on which were recovered to the normal situation five weeks after BH　extract and XKW were administered. Conclusion BH could effectively decrease the blood glucose and increase the insulin level of STZ-induced diabetic rats, and has effects to protect and recover the pancreatic island cells.

Objective To observe the effect of X-rays irradiation on IL-18 expression in mouse peritoneal macrophages. Methods Eiyghty Kunming white mice were divided into 10 groups(eight each group) : sham-irradiation, four groups of low-dose irradiation (0.05，0.075， 0.1 and 0.2 Gy), and five groups of high-dose irradiation (0.5，1.0，2.0，4.0 and 6.0 Gy). The mice were killed 16 h after whole body irradiation(WBI), with X-rays then the peritoneal macrophages were obtained and cultivated with culture medium of RPMI 1640 in culture box with 37℃，5％ CO₂ for 72 h. The supernate was gathered to detect the IL-18 expression level in macrophages by ELISA. Results The IL-18 expresson level in sham-irradiation group was (51.29±3.26 )ng•L^-1, IL-18 expression was enhanced after 0.05 Gy WBI, but there was no significant difference (P＞0.05）. The expression level was increased to (122.78±8.07) ng•L^-1 after 0.075 Gy WBI, and there was significant difference compared with sham-irridiation group(P<0.01). IL-18 expression was increased gradually with the enhancement of X-rays irradiated doses　（0.1， 0.2，0.5 and 1.0 Gy), and the values were (135.32±5.27)，(149.40±16.54)，(200.42±15.42)and(347.59 ±17.88)ng•L^-1, respectively. It peaked after 1.0 Gy irradiation, then it decreased after 2.0，4.0 and 6.0 Gy irradiation, and the values were (229.49±14.68)，(253.79±6.69) and (223.32±20.85 )ng•L^-1, respectively, but they were higher than that in sham-irradiation group(P<0.01). Conclusion Both high-dose and low-dose X-rays irradiation can stimulate IL-18 expression in mouse peritoneal macrophages.

Objective To evaluate the in situ biocompatibility of a new biodegradable ureteral stent made of L-lactide-glycolic acid copolymer 80:20(PLGA 80:20). Methods 16 dogs served as experimental animals. Ureteral stents of PLGA 80:20 were inserted in situ into the left ureter after transection at the mid level, then the ureters were routinely anastomosed. Ureters surrounding the stent were taken out 2,4,8 and 12 weeks postoperatively. The ureters were dissected to find changes of stents and local ureters. Histological analysis was performed to investigate tissue reactions to the stent and evaluate the biocompatibility. Rods of UROVISION stent served as controls. Results The PLGA stent was degraded completely within 12 weeks post implantation. In the early stage (2～4 weeks), both stents induced epithelial hyperplasia and inflammatory cell reaction at local ureter （P>0.05）. In the later stage (8～12 weeks), the tissue reaction nearly subsided in PLGA stented ureters after degradation of the device. Whereas, the tissue reaction induced by UROVISION stent had lasted throughout the observation period, even deteriorated with time going（P<0.05）. Conclusion The tissue reaction induced by PLGA stent is retrievable. PLGA is regarded highly compatible and can serve as an ideal material for biodegradable ureteral stent.

Objective To investigate the role of anion exchange in nonspecific phagocytic process of human retinal pigment epithelial (RPE) cells. Methods Fluorescent latex beads were incubated with RPE cells at 37℃, flow cytometry was performed on RPE cells that had been challenged with fluorescent latex beads. The quantity of phagocytosis was expressed as a phagocytic index. Experiments were also performed in the presence of different concentration anion exchange blocker DIDS（1，10，100，200，300，500 and 1 000 μmol•L^-1）.Results Phagocyticindex of RPE cells was (35.1±1.6)% at 6 h of incubation without DIDS. Phagocytic index of RPE cells was (34.1±2.1)% in the presence of 1 μmol•L^-1 DIDS（P>0.05）. Phagocytic index of RPE cells was (28.7±1.9)% in the presence of 10 μmol•L^-1 DIDS，10μmol•L^-1 DIDS significantly inhibited the nonspecific phagocytic process of human RPE cells（P<0.05）, and DIDS inhibited the process in concentration-dependent manner. Phagocytic index of RPE cells was (23.0±1.2)%，(17.9±1.5)%，(12.0±1.6)%，(7.0±2.2)% and (5.0±2.5)% in the presence of 100，200，300，500 and 1 000 μmol•L^-1 DIDS (P<0.01).Conclusion The inhibition of anion exchange activity will result in the decrease of nonspecific phagocytosis in RPE cells, anion exchange maybe participate in the nonspecific phagocytic process of RPE cells.

Objective To observe the action of Agaricus blazei Murrill ω-6 polyunsaturated fatty acid (ω-6APFA) on inhibiting the formation of thrombus and relieving arteriosclerosis. Methods Sixty rats were randomly divided into control group, natural soybean phospholipids capsule (NSPC) 0.70 g•kg^-1 •d^-1 group,ω-6APFA 0.07, 0.14 and 0.28 g•kg^-1•d^-1 groups (n=12). Rats in each group were exhibited once a day for 7 d,1 h after the last exhibition ,all rats were injected chloral hydrate 300 mg•kg^-1 in abdominal cavity to anesthetize, the carotid of the right neck was separated, and the time of thrombosis formation was determined by experimental intracorporeal thrombosis surveyor. Another 60 mice were randomly divided into control group, NSPC 1.0 g•kg^-1•d^-1group,ω-6APFA 0.1, 0.2 and 0.4 g•kg^-1•d^-1 groups (n=12). Mice were exhibited once a day for 7 d continuously, 1 h after the last exhibition, blood was extracted in the orbit vein, the clotting time was determined through glass slides method. Results NSPC 0.70 g•kg^-1• d^-1 and ω-6APFA 0.14，0.28 g•kg^-1•d^-1 had obvious inhibitory effect on thrombusformation in blood stasis rats, compared with model group(P＜0.05);andω-6APFA 0.14 and 0.28 g•kg^-1•d^-1 were superior to NSPC 0.70 g•kg^-1•d^-1. NSPC 1.0 g•kg^-1• d^-1 and ω-6APFA 0.2,0.4 g•kg^-1•d^-1 all could prolong the clotting time, compared with model group(P<0.05 or P<0.01),andω-6APFA　0.2, 0.4　g•kg^-1 •d^-1 were superior to NSPC 1.0 g•kg^-1 •d^-1. Conclusion ω-6APFA has the functions of inhibiting thrombusformation, promoting blood flow ability and lowering blood viscosity.

Objective To clone and analyze the MN/CA9 gene sequence in ICR mice and detect the expressions of MN/CA9 gene in various tissues of ICR mice. Methods The fresh tissues of small intesine, uterrus, skin, musle, liver, pancreas, heart, lung, thymus, spleen, kidney, ovary, stomach ,urinary bladder from ICR mice were obtained, the total RNA was extracted by GIT method, the 1st strand and 2nd strand of cDNA were synthesized, the EcoRⅠ adapters were lingated, the EcoRⅠ ends were phosphorylated, digested with XhoⅠ ,cDNA was ligated into the ZAP expression vector, packaged, planted, screened. The expressions of MN/CA 9 gene in various tissues in mice were detected by RT-PCR. Results A fragment of human MN/CA9 gene was used as probe, and 1.47×10³　clones were screened with radioactive isotopic ³²P labeled probe， after hybridizations, one positive signal of cDNA was detected and the complete nucleotide sequence contained 1 671 bp was determined （GenBank:AB086322）, The nucleotide similarity between mouse and human cDNA （GenBank:Z54349） was 69.1%.The MN/CA9 gene detected by RT-PCR assay (primer: P521-P1193) strongly expressed in small intesine,uterus, musle, pancreas, heart, lung, thymus, spleen, kidney, ovary, stomach,and urinary bladder,meanwhile did not express in skin and liver. Conclusion The expressions of MN/CA9 gene in some tissues of ICR mice are similar to that of human, it can be used to further functional analysis of MN/CA9 gene.

Objective To investigate the feasibility of using a new type carina type tracheal prosthesis made of complex material to replace trachea and carina defects. Methods According to biomechanics index, a new carina type tracheal prosthesis was designed and constituted with carbon fiber and silicon. 19 dogs were divided into two groups randomly: group A(n=11), the carina type tracheal prosthesis anastomosed directly to dog′s trachea and bronchus;group B(n=8), based on group A, meanwhile the diaphragm was incised and the pedicel caul was transplanted from abdominal cavity to thorax to enwrap the carina type tracheal prosthesis and anastomotic site.Results The average survival period post operation in group A and group B were (3.462±1.849)d and (6.475±1.062)d, respectively. There was significant difference of the survival period between two groups(P<0.05). The anastomose dehiscence: group A was（37.5±36.0）% ; group B was（16.0±25.0）%, there was significant difference (P<0.05). Conclusion Using the pedicel caul to enwrap the carina type tracheal prosthesis and anastomotic site can reduce the degree of anastomose dehiscence and prolong the survival period of the experimental dogs, but how to prevent the prosthesis shift and anastomose dehiscence will be still a most question to be settled.

Objective To investigate the degeneration of dopaminergic neurons induced by MPP⁺ in mouse mesencephalic dissociated culture. Methods OF1/SPF　mouse embroys of 14 d were used to make mecsencephalic dissociated culture, and the culture was divided into control and MPP⁺ -treated group. On the 10th day in vitro, different concen Trations of MPP⁺ (final concentrations of 0.1,1,10 and 15 μmol•L^-1) were added into the culture and incubated for 48 h. Dopaminergic neurons were identified and counted by using tyrosine hydroxylase (TH) immunochemistry under microscope. In order to investigate the time course effect of MPP⁺ on the degeneration of doapminergic neurons, 10 μmol•L^-1MPP⁺ was used and incubated for different time (2,4,8,24,48,72 and 96 h) on the 10th day in vitro. Results The numbers of dopaminergic neurons per well in the 0.1,1,10 and 15 μmol•L^-1 MPP⁺ groups were (70.30±6.38)%,(67.39±4.92)%,(51.68±2.95)%, and (50.91±5.60)% of control level, respectively. The cell death of dopaminergic neurons induced by 10 μmol•L^-1 MPP⁺ presented in two different ways. Loss of fibers and terminals of dopaminergic neurons was common way of damage, only few degenerated dopaminergic neurons presented as the loss of cell body. The number of dopaminergic neurons in the control group was (839.8±15.5) per well;meanwhile (677.2±6.1),(411.5±26.6),(229.0±20.3), and (191.3±15.2) were found in MPP⁺-treated groups 24, 48, 72 and 96 h after administration, respectively, which were lower significantly than that in control group（P<0.05）. Conclusion MPP⁺ with different concentrations（0.1,1,10 and 15 μmol•L^-1） could induce the damage and dead of dopaminergic neurons.The degeneration and death are both dose-and time-dependency. And two different mechanism may exist in the damage and death of dopaminergic neurons induced by MPP⁺.

Objective To observe the effects of purified product trihydroxybenzoic acid dimmer in different doses from Trapa manshurica Fler on apoptosis and cell cycle progression of liver cancer SMMC-7721 cells in vitro and explore its possible mechanism of inhibiting tumor growth. Methods The changes of apoptosis and cell cycle progression were measured with flow cytometry. Results The apoptotic percentages of the cells treated with the purified products with doses of 3.125, 6.250, 12.500 and 25.000 mg•L^-1 increased significantly as compared with that in the control (P<0.001). And the percentages of G₂+M phase cells increased, those in 3.125, 6.250 and 12.500 mg•L^-1 groups were significantly higher than that in the control (P<0.01 or P<0.05); while the percentages of S phase cells decreased，especially decreased in 6.250, 12.500 and 25.000 mg•L^-1 group(P<0.05). Conclusion The inhibitory effect of purified product trihydroxybenzoic acid dimmer from Trapa manshurica Fler on tumor growth could be due to cell apoptosis caused by G₂+M phase arrest.

Objective To study the effects of MT egg-milk powder on the contents of lead,zinc,iron and copper in brain and femur of lead poisoning rats. Methods 30 Wistar rats were divided into three groups(n=10): normal control group, lead poisoning group and lead poisoning rats treated with MT egg-milk powder group. After treated with 0.2% lead acetate daily for three weeks, the rats were fed with 500 g•L^-1 MT egg-milk powder for three weeks. At the end of the experiment，samples of brain and femur were collected for trace elements detection by atomic absorption spectroscopy. The concentration of hemoglobin was determined by haemiglobincyanide method.Results Compared with the control, the contents of zinc and hemoglobin in brain and femur were significantly decreased (P<0.05), but the contents of lead and iron in brain and femur were significantly elevated（P<0.01）in lead poisoning rats. After the lead poisoning rats were exposed to MT egg-milk powder, the content of lead was decreased (P<0.05),the contents of hemoglobin and copper in both brain and femur were obviously increased（P<0.01）.Conclusion MT egg-milk powder can obviously improve the metabolism disorder of trace elements in lead poisoning rats.

Objective To observe the distribution and location of TGF-β₁ in different stages of experimental lesions and to discuss its potential function.Methods Thirty Wistar rats were established experimental periapical lesions in maxillary first and second molar as experimental group while mandibular molar as control group, and they were divided into ten groups by the time（1，2，3，4，5，6，7，8，9, and 10 weeks）after pulp exposure, three rats in everygroup. Expression of TGF-β₁ was detected by histopathology and immunohistochemistry.Results Strong expression of TGF-β₁ was observedin acute inflammation of pulp (1st-2nd week) and periapical tissue (4th-6th week), and idisappeared in pulp aecrosis and in chronic inflammation of periapical tissue(7th week). The main cells expressing TGF-β₁ were macrophage，lymphocyte and fibroblast. Conclusion TGF-β₁ has the certain function in inflammation and prevention of experimental periapical lesions.

Objective To study the inhibitory effects of N-aetyl-cysteine(NAC) on the expressions of matrix metalloproteinases (MMPs) of macrophage-derived foam cells (FCs). Methods The hypercholesterolemic (0.5% cholesterol and 4.5% coconut) rabbit model of atherosclerosis was used to investigate the gelatinolytic activity associated with FCs，which were isolated from rabbit atheroma. FCs were maintained in culture medium for 24 h up to 4 d, NAC (10 mmol•L^-1) was added to culture medium of FCs.The expresions of MMPs were detected with SDS PAGE and immunohistochemistry assay.Results The Pro-MMP9 concentrations in NAC group at 24 h,48 h and 4 d［(654±33.5)，(780±65.0) and (799±48.9)INT•mm^-2］were lower than those in control group［(1455±50.9),(15 615±52.0) and (1 467±48.8)INT•mm^-2］ (P<0.01).At 24 h,48 h and 4 d the concentrations of MMP2 in NAC group ［(221±25.3),(224±25.0) and (202±33.9) INT•mm^-2］were lower than those in control group［467±34.3),(651±42.0) and (671±34.8)INT•mm^-2］(P<0.01),the immunohistochemical result showed MMP2(+ ), macrophage(+), and MMP9(+).Conclusion This newly described action of antioxidant therapy might be useful to inhibit matrix degradation and to improve vascular stability. NAC represents an attractive therapeutic target.

Objective To study the effects of low dose radiation（LDR）on apoptosis of transplantation tumor cells of human glioma (U251)-bearing nude mice.Methods Thirty-five tumor-bearing nude mice were divided into seven groups(n=5):sham-irradiated group,0 mGy；D₁ group,75 mGy；D₂ group,4 Gy；D₁-12 h+D₂ group,the time between D₁ and D₂ was 12 h; D₁-24 h+D₂ group,the time between D₁ and D₂ was 24 h；D₁-48 h+D₂ group,the time between D₁ and D₂ was 48 h；D₁-72 h+D₂ group,the time between D₁ and D₂ was 72 h. The apoptosis of transplantation tumor cells of tumor(U251)- bearing nude mice was detected by TUNEL. Results There were TUNEL positive cells in all groups. The apoptotic index (AI) in 75 mGy group(0.19±0.07) was slightly increased, but the difference was not significant compared with sham-irradiated group(0.17±0.07)（P>0.05）.While in D₂ and D₁+D₂ groups, the AI (0.54±0.05,0.64±0.04,0.63±0.04,0.65±0.11,0.67±0.07) were increased, and the latter was more obviously. There were significant differences compared with sham-irradiated group（P＜0.01）.Conclusion LDR may promote apoptosis of transplantation tumor cells to some extent ,and could enhance high dose radiation to induce apoptosis of transplantation tumor cells.

Objective To investigate the possibility and mechanism of ¹²⁵I in treatment of glioma. Methods SHG-44 glioma cells were cultivated in vitro, the inhibitory effect of¹²⁵I on SHG-44 cell proliferation was determined by MTT method. The stereotactic method was used to establish the rat intracranial glioma model. The MRI was performed at 1st week after implantation and ²⁵I was implanted in the glioma area, the MRI was performed to measure the diameter of tumor 2 weeks after implantation. The rats were killed after 2 weeks ,PCNA gene experession was determined with immunohistological method both in control and experiment group.Results one week after implantation the glioma grew,the results of MTT method showed the growth of the SHG-44 was inhibited, ¹²⁵I inhibited the expression of PCNA gene and enlonged the rat survival period. Conclusion ¹²⁵I can inhibit the growth of glioma ,the mechanism may be concerned with its inhibitory effect on PCNA gene expression.

Objective To investigate the effects of zinc on apoptosis of prostate cancer cells(PC-3M). Methods The cell proliferation was determined by MTT assay treated with different concentrations of ZnSO₄•7H₂O(end concentrations were 6.25，15，25，50，100，150，200，250，and 300 μmol•L^-1,respectively), the cell morphological changes after exposed to zinc for 24 h was observed under phase-contrast microscope, the apoptosis was detected by AO/EB assay, the cell cycle and apoptotic peak were detected by flow cytometry, DNA fragmentation was observed by agarose gel electrophoresis(AGE).Results PC-3M cells proliferation rate was obviously changedafter exposed to different levels of zinc for 24 h. Zinc(250 μmol•L^-1) inhibited proliferation of PC-3M cells (P<0.01). Compared with control cells, the changes of morphological characteristics of PC-3M cells were found,including shrank and floated,decreased refraction in the cells exposed tozinc (250 μmol•L^-1) for 24 h. The confocal microscopy showed the changes of cells through AO/EB staining.The cellular membrane was integrity but vacuolization of the cytoplasm,chromatin was flavo-green, nuclear condensed, nuclear side collection, caryolysis, nuclear fragmented. The flow cytometry showed the hypodip-loid peak in G₀/G₁ phase after exposed to zinc (250μmol•L^-1)for 24 h. It had visible DNA fragmentation by AGE.Conclusion Zinc with high concentrationcan inhibit human prostate cancer cell growth and induce apoptosis of human prostate cancer cells.

Objective To explore the effect of Acanthopanax senticosus injection (ASI) on brain multi-infract dementia rats. Methods Thirty-six male Wistar rats were randomly divided into 3 groups:control group, model group and ASI group. The multi-infract dementia rat mo dels were set up by injecting mini-sludged blood in carotis internal arteries, learning and memory function of rats were determined by Morris water maze and Step-down test, the section of rat cerebral cortex and hippocampus were stained with haematoxylin eosin (HE).Results Compared with model group, latency in ASI group shortened significantly at 2nd,5th and 6th day, the distance shortened at 1st,2nd,5th and 6th day. The seeking tactics of ASI trgated rats improved in the Morris water maze test. The error times of ASI treated rats decreasd at 1st and 2nd day in Step-down test; ASI did not reduce significantly pathological changes of vascular dementia rats. Conclusion ASI has effect of treatment on multi-infract dementia rats.

Objective To study the β-catenin gene expression and inhibitory effects ondiffused malignant plural mesothelioma tissues. MethodsB NCI-H28 cell apoptosis was detected with the techniques of Annexin V and TUNEL.The inhibitory effect of β-catenin was observed with incubation in vitro.Results The cells transferring wild β-catenin（β-CAT-V5-FP）and mutant β-catenin （β-CAT-S37C-V5-FP）were increased signifcantly compared with blank carrier group. The β-catenin gene transferring carboxyl absent also resulted in apoptosis, but the apoptotic rate was lower than those of cells transferring wild β-catenin（β-CAT-V5-FP）and mutant β-catenin （β-CAT-S37C-V5-FP）. Conclusion β-catenin gene can induce apoptosis on non-epithelioid tumor-diffused malignant plural mesothelioma.

Objective To evaluate the inhibitory effect of the polyI： C on subcutaneous prostate carcinoma of mice. Methods The mouse prostate carcinoma models were established by inoculating RM-1 cells to subcutaneous tissue of mice. Then the mice were randomly divided into two groups: control group and polyI：C group according to tumor weight(n=8) .After seven times′ treatment, the prostate carcinoma was separated from mice and the tumor and mouse weight were measured and the inhibitory rates of polyI：C was calculated. The tumor growth curves and mice survival curves were drew. HE staining was used to evaluate the morphological changes of prostate carcinoma tissue.Results The tumor weights in control and polyI：C group were (4.14±1.56) and　(1.58±0.67)g ,respectively,and there was significant difference between two groups(P<0.05). The tumor inhibitory rate of polyI：C was 67.85%.　The tumor growth curves showed that the growth of polyI：C group was slower than that in control and the mouse survival curves showed that there was significant difference of mouse survival time between two groups　(P<0.05).HE staining showed that the cell proliferation in control group was more active than that in polyI：C group. Conclusion PolyI：C can strongly inhibit not only the growth but the migration rate and death rate of prostate carcinoma.

Objective To investigate and evaluate the curative effect of radioimmunological targeting drug on nude mice bearing breast cancer. Methods The anti-CEA monoclonal antibody C50 was combined with ¹³¹I to produce radioimmunological targeting drug. 16 nude mice inoculated subcutaneously with breast cancer cell MCF-7 with tumor diameter about 0.5 cm were randomly into 4 groups(n=4): group Ⅰ, injected in part with ¹³¹I-C50 18.5 MBq; group Ⅱ, injected in part with ¹³¹I-C50 3.7 MBq; group Ⅲ, injected in part with¹³¹I-mIgG 18.5 MBq; group Ⅳ, injected in part with C50 0.75 μg. The size of tumor volume and inhibitory rate (IR) after treatment for six weeks were calculated and compared with the control group. Results The tumor volume and curves for tumor growth and tumor weight had significant differences between group Ⅰ and the group Ⅲ as well as group Ⅱ (P<0.01); and there was significant difference between group Ⅰ and group Ⅱ (P<0.05), there was no significant difference between group Ⅲ and group Ⅳ (P>0.05). Conclusion Anti-CEA monoclonal antibody C50 labeled with radionuclide ¹³¹I could inhibit the growth of the tumor when given locally. ¹³¹I-C50 has a potential value of clinical application .

Objective To investigate the effects of pancreatic kininogenase on pressure,myocardical fibrosis and serum nitric oxide (NO) level in spontaneously hypertensive rats (SHR). Methods Twenty-four (fifteen weeks) male SHR were randomly divided into three groups:SHR group,pancreatic kininogenase group and captopril group（n=8 ），8 male Wistar kyoto rats with normal blood pressure were considered as control group. Pancreatic kininogenase was given by peritoneal injection (7.2 U•kg^-1•d^-1), captopril was given by intragast ric administration（10 mg•kg^-1•d^-1）, the rats in SHR group and rats with normol blood pressure in control group were treated with 0.9% NaCl（2 mL•kg^-1•d^-1）administered through peritoneal injection. After four-week experiment, the pressure was measured in rats througth carotid artery,then the rats were sacrificed , and LVMI,CVF,PVCA and serum NO level were measured. Results In SHR group, SBP,LVMI,CVF and PVCA were higher, serum NO level was decreased obviously than those in control group (P<0.05). After treatment of pancreatic kininogenase,the serum NO level raised obviously,other indexes obviously reduced than those in SHR group (P<0.05),but except SBP,there were no significant difference compared with captopril group (P>0.05). Conclusion Pancreatic kininogenase can obviously reduce the blood pressure and reverse myocardial fibrosis,its mechanism may be concerned with the increasing of NO level in SHR.

Objective To study the anti-ageing and antioxidation of polyphends of vitis amurensis Rupr(PVAR).Methods Forty senile male Wistar rats (20 months) were divided into 4 goups according rats′ weights:senile negtive control group(A), fed with basic forage;two senile PVAR groups(B and C),fed with basic forage inchuding 200 and 400 mg•kg^-1•d^-1 PVAR;senile positive control(D), fed with basic forage inchuding 200 mg•kg^-1•d^-1 melissic powder.After fed continuously for 8 weeks,the MDA contents in RBC-membrane, plasma and liver,sulfhydryl content in RBC-membrane, sialic acid content,activity of Na⁺-K⁺-ATPasc,fluidity of RBC-membrane,and activity of plasma GSH-Px in rats were detected.Results Compared with group A,the contents of MDA in groups B,C,and D decreased significantly(P<0.05，P<0.01)；sulfhydryl contents in RBC-membrane and activities of plasma GSH-Px in group C and D increased significantly(P<0.05).There were no significant differences of various indexes between group B,C and group D (P<0.05).Conclusion PVAR has anti-ageing and antioxidation on senile rats.

Objective To study the dose distribution of different bolus after different energy electron beam irradiation to different chest wall radiotherapy for the patients with breast cancer. Methods The paper simulated the dose distribution of women's left breast cancer after radical mastectomy by 6 and 9MeV electron beam irradiation, and TLD was used to measure. Results The dose of skin became higher and the dose of lung was less when 0.5 and 1.0 cm bolus were used on the body；with the increasing of the energy of electron beam, the high dose field became larger；and with the same energy of electron beam, the high dose field moved to surface of the body when the bolus was thicker.Conclusion When different energy electron ray irradiates different thickness bolus ,the dosage of skin surface increases and the dosage of anterior margin of lung reduces. With electron ray energy increasing ,the high dosage field is widen ,when the electron ray energy is identity ,the high dosage field migrates to the surface after adding bolus.Using certain depth bolus may attain the therapeutical dose of target area.

Objective To study the changes of biochemistry indexes in blood serum and bone mineral density (BMD) of secondary osteoporosis after spinal cord injury (SCI) in rats. Methods 120 male Wistar rats were divided randomly into 12 groups (n=10). The rats in control groups were only removed larmina with dura intact, without spinal cord injury at the level of the tenth thoracic vertebrae, the rats in experimental groups were also removed larmina, then paraplegic model was made by the method of Allen's (60gcm). The changes of biochemistry indexes in blood serum and BMD were observed. Results Compared with control groups, the concentrations of alkaline phophatase (ALP) were decreased at the 1st and the 3rd week in experimental groups (P<0.05), increased significantly at the 7th week in experimental groups (P<0.01). the concentrations of phosphorus were increased at the 1st and the 2nd week in experimental groups (P<0.01); the concentrations of calcium were increased at the 1st ， 2nd and 3rd week in experimental groups (P<0.01); then the concentrations of calcium and phosphorus declined to a little higher than normal. Compared with control group, the BMD of humerus were decreased at the 3rd, 7th and 11th week in experimental group (P<0.01), especially at the 3rd week; BMD of femur was decreased at the 7th week in experimental group (P<0.01), there was no difference between the 11th week group and control group (P>0.05). BMD of tibia was decreased at the 7th and 11th week in experimental group (P<0.01), but there was no difference between the 7th and the 11th week in experimental groups. Conclusion The changes of biochemistry indexes in blood serum fluctuated after spinal cord injury can be found , osteoporosis is found in bones both superlesion and sublesion, and bone superl esion rehabilitate early in paraplegic rats

Objective To investigate the genetic relationship between the cytosolic phospholipase A2 (cPLA2) gene polymorphism and type 2 diabetes mellitus (T2DM) in Northern Han Chinese. Methods The method of PCR- RFLP was conducted to examine the genotype of 3 cPLA2 genes ( PLA2G4A, PLA2G4B and PLA2G4C genes) in 263 subjects, including 121 cases of T2DM and 142 controls. The conditional test was used to test the combined effect of distinct loci on the T2DM by conditioning on allele (COA) or by conditioning on genotype (COG) with UNPHASED analysis platform. Results The genotypic frequencies of the 3 genes did not deviate from Hardy-weinberg equilibrium in both case and control groups. The allelic and genotypic frequencies of the 3 genes had no remarkable difference between case and control (P>0.05). The COG test revealed that T2DM was associated with the rs1648833-rs1549637 combination (P<0.05), but the COA test did not show such an association with the combination. Neither the COA test nor the COG showed that T2DM was associated with the BanISNP-rs1648833 and BanISNP-rs1549637 combination. In the combination of all 3 SNPs (BanISNP-rs1648833-rs1549637), the COA test and the COG test didn′t show an association (P>0.05). Conclusion cPLA2 gene polymorphism may be involved in the etiology of T2DM in Northern Han Chinese, although their effects are relatively small.

Objective To investigate the changes of CD4⁺CD25⁺ regulator T cells in peripheral blood from the paitents with lung cancer and breast cancer. Methods Flow cytometry was used to analyze the proportion of CD4⁺CD25⁺ T cells/CD4⁺T cells in peripheral blood mononuclear cells (PBMC) from 9 patients with lung cancer, 9 patients with breast cancer and 10 patients without cancer as controls. The expression of foxp3 mRNA in PBMCwas detected by RT-PCR. Results The value of CD4+CD25+ T cells/ CD4+T cells in PBMC from patients with lung cancer and breast cancer (21.30±11.26)% was significantly higher compared with controls (14.19±〖JP〗8.56)%. The expression of foxp3 in PBMC enhanced in patients with cancer compared with controls，there was significant difference between two groups (P<0.05). It revealed that the population of CD4⁺CD25⁺ regulator T cells in peripheral blood of patients with cancer was increased. Conclusion CD4+CD25+ regulator T cells may be responsible for immune suppression in the tumor immunity while maintenance of immune tolerance and the increase of CD4+CD25+ regulator T cells may be involved in tumor generation.

Objective To study the biological effects of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in invasion and metastasis of prostate carcinoma. Methods Immunohistochemistry and double-immunofluorescence techniques were used to detect the expressions of MMPs and TIMPs in 5 normal prostate and 34 prostate carcinoma tissues. Results Immunohistochemistry showed that the expressions of MMP7 and MMP9 increased with malignant degree of prostate carcinoma (P<0.001). TIMP1 and TIMP2 expressions had not significant difference between normal prostate and prostate carcinoma (P>0.05). Immunofluorescence staining showed that MMP13 expressed in membrane of normal prostate cells and cancer cells. Double immunofluorescence staining showed that MMP7 and LN both expressed in cancer cells, basement membrane, smooth muscle cells and nerve fiber. MMP9 and collagen Ⅳ both expressed in basement membrane. Conclusion MMP7 and MMP9 can be used as markers for invasion and metastasis of prostate carcinoma . MMP13 has nothing with malignant degree of tumor. In different grades of prostate carcinoma , TIMPs and MMPs have different changes. 

Objective To investigate the application of PSA, fPSA concentrations and fPSA/PSA value for diagnosis and therapy of prostatic diseases detected with dissociation enhanced lauthanide fluoroimmunoassay (DELFIA) technique. Methods Thirty-four cases of normal individuals were used as control group, 46 patients with prostatitis as prostatitis group, 123 patients with prostatic hyperplasia as BPH group and 39 patients with prostate cancer as prostate cancer group. PSA, fPSA concentrations and fPSA/PSA value were detected with DELFIA technique. Results In prostate cancer group, the PSA and fPSA concentrations were significantly higher than those in other 3 groups (P<0.01),as fPSA/PSA value was remarkably lower than those in the other 3 groups (P<0.05).However, the fPSA/PSA values in other 3 groups demonstrated no significant difference (P>0.05).Conclusion DELFI A technique is an effective method to improve the diagnosis and differential diagnosis of prostatic diseases, especially for prostate cancer.

Objective To observe the effect of mifepristone on ultrastructure of fetal kidney in the second trimester pregnancy. Methods Twenty five normal pregnant women whose pregnancy ranged from 16-28 weeks were randomly divided into 2 groups: control group(n=10) and test group（ n=15 ）.The women in control group were given waterbag to induce labor alone. Test group was divided into 2 groups (test group Ⅰ：n=10, gestation of 16-22 weeks; test group Ⅱ：n=5, gestation of 23-28　weeks). Test group took mifepristone 100 mg orally per day for 3 d, then were given waterbag to induce labor. After delivery of fetus, fetal kidney was separated immediately for observation by transmission electron microscopy.Results In test group Ⅰ, Glomerulus′s cell was swelling and cavitation. Mesangial cell nuclei showed irregular shape and mitochondria swelled, stroma was normal. The thick endothelial cytoplasm, imperforation, thin basal membrane and podocyte matrix cavitation, villus-like podocyte, secondary enation,proximal convoluted tubule nuclei cavitation were found in filtration membrane, intracytoplasm was vacuoles and had lysosomes. Some nuclei and kytoplasm had cavitation in distal convoluted tubule. In test group Ⅱ, Glomerulus′s cell cavitation was seen. Hyperplasia look like arch local. Little mesangial cell nuclei showed irregular shape or light pycnosis. Mitochondrion in cytoplasm was mild swelling, crest fragmentated partly. The cytoplasm showed mild swelling, podocytes were normal, primary and secondary enation, local fusion and mild villus were found in filtration membrane ,proximal convoluted tubule and distal convoluted tubule were normal. Conclusion Mifepristone could take ischemia hypoxia changes in the ultrastructure of fetal kidney in the second trimester pregnancy. The earlier the stage of the fetus is, the more obvious the ultrastructural changes are.

Objective To investigate the association between angiotensin Ⅱ type 1 receptor (AT₁R) gene polymorphism and hypertensive disorder complicating pregnancy （HDCP）. Methods Eighty-six pregnant women with HDCP and 74 normal pregnant women (control group) were recruited. Gene polymorphism of AT₁R at (-535)was detected with PCR-based restriction fragment length polymorphism(RFLP)method. Results ① The frequencies of CC,CT,TT genotypes of AT₁R at (-535) were 74.4%,20.9%,4.7% in HDCP group and 87.8%,8.1%,4.1% in control group,respectively;there was no significant difference berween two group（P>0.05）.The frequencies of the two alleles C, T were 84.9%,15.1% in HDCP group and 91.9%,8.1%in control group,respectively;there was no significant difference between two group （P>0.05）;②The distribution frequencies of AT₁R at(-535) in HDCP group in gestational hypertension,mild preeclampsia,severe preeclampsia(5.9%,7.1%,15.2%)were lower than that in eclampsia in HDCP group（P<0.05）.Conclusion The AT₁R (-535) C→T gene polymorphism is not associated with the pathogenesis of HDCP, but it has an association with the severity of HDCP.

Objective To provide theoretical support for mandibular rigid internal fixation. Methods One of adult male complete dentition was chosen, and then using Frankfort horizonal plane as benchmark , CT scanning was done from condylar process to mental region,73 lamella in all.Using Photoshop 7.0 analysis bone tissue window fault, and coordinate value was load into Solid Works 2002 definite software ,then in a three-dimensional computer model of mandible, fracture mobility and plate strain were calculated for bite forces applied on incisors, fracture side and non-fracture side first molars. Simulated mandibular body and symphysis fractures were rigid internal fixed with two titanium miniplates. Fracture without interfragmentary contact were study. The loads were transmitted only through the plates and none stress was transmitted through the fracture surfaces. This computer-based study with finite element analysis (FEA) was done to estimate the stability of tention band fixation of mandibular fractures. Results With two miniplates, the symphysis and body fracture mobility were lower than 150 μm. To mandibular body fractures which had angle of more than 20 degrees with mesial surface, at both the incisors bite force and fracture molar force situation, the fracture mobility was also lower than 150 μm, but the fracture mobility above 150 μm was calculated at non-fracture molar bite forces. Conclusion From a mechanical point of view, based on the FEA model, two titanium miniplates are suitable for symphysis and body fractures. However two miniplates can not provide sufficient stabilizations for mandibular body fractures which have angle of more than 20 degrees with mesial surface . Suitable indications should be selected when miniplates are fixed mandibular angle fractures.

Objective To study the effects of changes of neuropeptide Y(NPY) and neurotensin(NT) concentrations in plasma and their ratio imbalance on the incidence of essential hypertension (EH).Methods NPY and NT in plasma in 176 patients with EH were measured with radioimmunoassay(RIA). Results NPY in the EH patients was higher than that in the normal subjects (P<0.01), and went up with the aggravation of the patients′condition( P<0.01).NT in the EH patients was lower compared with the normal subjects (P<0.01), and went down with the aggravation of the patients′ condition ( P<0.01). NPY/NT in the EH patients was higher than that in the normal subjects (P<0.01), and was accordant with patients′ condition. Conclusion NPY and NT are involved in the incidence of EH.The ratio imbalance of NPY/NT may be one of the factors causing EH.

Objective To compare the apoptotic rates of sperm in patients with oligozoospermia，asthenozoospermia，teratozoospermia. Methods Semen parameters were determined by computer assisted sperm analysis (CASA). Semen samples were from 14 fertility persons and 48 infertility patients, of which 11 patients with oligozoospermia( sperm concentration<20×10^６•mL^-1), 25 patients with asthenozoospermia (a+b<50%) and 12 patients with teratozoospermia(percentage of normal morphology sperm<14%).The apoptotic rates of sperm in two groups were analyzed by Wright-Giemsa staining. Results The apoptotic rates of sperm in infertility and oligozoospermia groups were significantly higher than that in fertility group (P<0.05). The apoptotic rates of sperm were not significantly different between asthenozoospermia and teratozoospermia groups (P>0.05). The apoptotic rate of sperm in abnormal sperm concentration (sperm concentration<20×10^６•mL-1)group was significantly higher than that in nomal group (P<0.05). No significant difference of apoptotic rate of sperm of a+b grade motile sperm was found between the normal group and abnormal group (P>0.05). The apoptotic rate of sperm was not significantly different between the normal morphology sperm group and abnormal morphology sperm group (P>0.05). Conclusion The apoptotic rate of sperm in infertility group is significantly higher than that in fertility group, specially in oligozoospermia patients.

Objective To ensure the proportion of bronchiectasis patients with reversible airflow limitation and their responsibility to the bronchodilatator and glucocorticosteroid. Methods The basic pulmonary function, PEF variability measurement and the experiments of bronchodilatation were performed in 48 patients who had been diagnosed for bronchiectasia by HRCT . The positive patients were divided into three groups randomly:group A,the routine treatment group(n=11);group B, the routine treatment +β₂-agonist-inhalation group(n=11);group C, the routine group +β₂-agonist-inhalation + glucocorticosteroid-inhalation group(n=12). Results Among the 48 cases, 31 cases were positive in bronchodilatation experiments ,the PEF variability of 26 cases was higher than 20%,and 34 cases(78.83%) had the reversible airflow limitation. There were 8 cases(72.73%) with the PEF variability lower than 20% in group B while only 5 cases(45.45%) in group A one week after treatment, the difference was significant between group A and group B(P<0.01).The number of days in hospital in group B［(12.18±1.94) d］ was shorter than that in group A［(15.58±2.64) d ］(P<0.01). There were 10 cases (83.33%) with the PEF variability lower than 20% in group C, there was significant difference compared with group B (P<0.01).Compared with group B,the quantity of sputum draining per day and the days in hospital shortened obviously in goups C (P<0.01). Conclusion β₂-agonist and glucocorticosteroid should be inhaled in patients with bronchiectasia and reversible airflow limitation to block the airflow limitation and decrease the quantity of sputum draining,and they are also beneficial to the control of infection.

Objective To study the effects of Ozagre on CD62p,CD61, which was used as platelet marks and von willebran factor (vWF) in patients with acute coronary syndrome (ACS),and meanwhile to study the relationships between CD62p,CD61 and vWF. Methods CD62p ,CD61 from whole-blood samples were measured by FCM and vWF was measured by ELISA method in 36 patients with ACS and 20 normal peoples used as control group. Results The amount of expression of CD62p was（89.60±6.98）% in ACS patients group and （40.88±7.56）% in control group, there was obvious difference between ACS group and control group (P<0.05). The amount of CD62p was decreased to (66.11±8.72)% after treated with Ozagre (P<0.05). There was also significant difference of CD62p in ACS group between before and after intervention of ozagre (P<0.05). The amount change of CD62p in patients with ACS was agreement with the improvement of angina pectoris and the changes in sum of ST segements. However there was no difference of CD61 between ACS group and control group (P>0.05).There was also no difference of CD61 between before and after treatment of Ozagre(P>0.05). Conclusion CD62p is a sensitive index of platelet activation in ACS patients and there is excellent relationship between CD62p and vWF and clinical symptoms.

Objective To estimate the prevalence of human papillomavirus（HPV）infection and associated risk factors in men attending a sexually transmitted disease (STD) clinic. Methods A cross-sectional study was conducted with male STD clinic participants who had no genital warts. A personal interview was done and material for anogenital HPV DNA detection was obtained with swabs. HPV DNA was detected by polymerase chain reaction (PCR). Results The positive rate of HPV DNA among 600 subjects was 14.50%(87/600).The positive rate among STD patients (29.30%) was significantly higher than that of the subjects without STDs (11.11%, P<0.05). The most important predictors of HPV DNA positivity were younger age at first sexual intercourse (OR=1.2, 95%CI 1.1-1.3, P<0.05)and a history of condyloma acuminatum (OR=3.3, 95%CI 1.2-9.3, P<0.05) or other STDs in the past two years(OR=3.8, 95%CI 1.4-10.2, P<0.05), while other factors including age , preputial status, number of sex partners, the average frequency of sexual intercourse , condom using and sex partners' history of genital warts in the past two years had no significant relation with latent HPV infection. Conclusion Asymptomatic HPV infection in anogenitalia of men is prevalent, and this infection is more common in STD patients than in patients without STDs. The risk factors of HPV persistent infection includes younger age at first intercourse , history of genital warts or other STDs.

Objective To assess the efficacy and safety of inhaled corticosteoids for childhood asthma．Methods According to the requirements of Cochrane systematic review,a thorough literature search was performed among PubMed，China National Knowledge Infrastructure，Wanfang Date，and so on.With RevMan 4.2 software,a Meta-analysis was performed on a total of 1 994 children involved in 19 papers which met with the inclusion criteria．Results The literatures of total effective rates had homogenicity.In comparison of the treatment groups and the control，the incorporation was statistically significant［OR＝8.27，95%CI(5.20, 13.16)］．The literatures of serum osteocalcin levels after the treament for 6 and 12 months and growth rates had heterogeneity，and that of serum ACTH levels had homogenicity．Comparing the treatment group with the control group，the incorporation of the growth rates had statistical difference，while the other three incorporations did not．Sensitivity analysis showed that the results of total effective rates，serum osteocalcin levels after the treament for 12 months，growth rates and serum ACTH levels were stable and reliable，but that of serum osteocalcin levels after 6 months treament required further approach．The results of publication bias showed that the impact was small，and the results was reliable．〖WTFZ〗Conclusion The clinical evidences available so far indicats that the treatment of childhood asthma with inhaled corticosteroids is safe and efective，although rigorous designe and large sample size randomized double-blind clinical trials are required to further analysis for more stable and reliable results．

Objective To observe the effect of coronary artery disease on QT dispersion (QTd). Methods The QTd and QTcd in 245 patients underwent percutaneous coronary angiography (CAG) were analyzed. It was evaluated The relations between the QTd and the degree of coronary stenosis and the number of disease vessels. Results The QTd and QTcd were significantly prolonged in patients with coronary artery stenosis than those in patients with normal coronary artery(P<0.001). The QTd and QTcd also showed difference between patients whose coronary artery stenosis were ≥70% and patients whose coronary artery stenosis were <70% and ≥50% (P<0.01). The QTd was positively correlated with coronary stenosis degree(r=0.84，P<0.05). The seriouser the degree of coronary stenosis was, the greater the QTd was. The QTd and QTcd of the patients with single and multiple vessel lesion showed no significant difference(P>0.05). Conclusion There is no relation between the number of disease coronary artery and the QTd.

Objective To discuss the significance of VCE smear and the Beth esda 2001 report system in screening of cervical carcinoma. Methods 3056 healthy examination population were examined for cervical-vaginal cytology by VCE smear and the Bethesda 2001 report system. All of the cases were biopsied by coloscopy including atypical squamous cells of undetermined significance(ASC-US), squamous intraepithelial lesions and carcinoma(SIL+).Results 66 cases of SIL+ were found in cervical-vaginal cytology (2.15%), including 17 cases of atypical squamous cells with undetermined significance(ASC-US) (25.76%), 4 cases of atypical squamous cells, didn't exclude HSIL(ASC-H)(6.06%), 28 cases of low-grade SIL(LSIL)(42.42%), 14 cases of high-grade SIL(HSIL)(21.21%), 3 cases of squamous cervical carcinoma(SCC)(4.55%). In 66 cases of cervical-vaginal cytology positive results, 60 cases of CIN I+ were confirmed by biopsy pathology, including 35 cases of CIN Ⅰ(53.03%), 12 cases of CIN Ⅱ(18.18%), 7 cases of CIN Ⅲ(10.61%). Conclusion VCE smear and the Bethesda 2001 report system can get cells conveniently and the results are more correct.The coincidence of the examination of cervical-vaginal cytology and pathology is 90.91%. VCE smear and the Bethesda 2001 report system has great significance in screening of cervical carcinoma.

Abstract:Objective To explore the diagnostic value of integrated backsca tter (IBS) technique for carotid artery atherosclerosis (AS) in hyperlipemia patients .Methods The calibrated IBS (C-IBS, C-IBS=intima-media IBS-adventitia IBS) was used to detect the carotid intima-media in the different section in 51 normal controls and 98 patients with hyperlipemias. Results The C-IBS in hyperlipemia group was lower than that in normal control, there significant difference of C-IBS between carotid sinus artery( CSA) and internal carotid artery (ICA)(P<0.05), the C-IBS of common carotid artery(CCA) in hyperlipemia group was lower than that in normal control, but no significant differentce(P>0.05).In hyperlipemia group ,there were significant differences of C-IBS between CCA, CSA and ICA (P<0.05); and there were no significant differences of C-IBS between CSA and CCA, CSA and ICA (P>0.05). Conclusion IBS technigue can reflect the changes of tissue structure of vascular wall and provide a new ,no invasive and feasible method to evaluate AS early.

Abstract:Objective To evaluate the values of integrated backscatterr (IBS) and dobutamine stress echo- cardiography (DSE) in the diagnosis of coronary artery disease(CAD).Methods Thirty-one suspected CAD patients received DSE and IBS analysis before selective coronary angiography(SCA).Using SCA results as the golden standard of the diagnosis,the diagnostic sensitivity(Se),specificity(Sp),and accuracy rate (Ac) of conventional eye-view and DSE-cyclic variation integrated backscatter (CVIB) were compared. Results The CVIB of myocardial segments perfused by stenotic coronary artery was smaller than that of segments perfused by patent coronary artery during high dose of DSE. For conventional DSE eye-view method, the Se,Sp,and Ac were 80%,72% and 77%, while that of DSE-CVIB were 90%,72% and 83%, respectively. Conclusion IBS is a valuable method in assessing the myocardial wall motion in DSE, and the combination of the two methodscan improve the diagnostic accuracy rate of CAD.

Objective To study the clinical efficacy of differential diagnosis between malignant and benign solitary pulmonary nodules (SPNs) with single-location dynamic enhanced computed tomography (CT). Methods Thirty-nine patients were studied with single-location dynamic enhanced CT. Patterns of time-density curve were drawn. Peak height and increment of CT value were calculated. 〖WTFZ〗Results Malignant and benign SPNs showed quite different patterns in time-density curve. Enhanced degree of pulmonary carcinoma ［(41.9±2.8）Hu］was higher than that of the tuberculosis［（11.7±7.85)Hu］, which had significant difference (P<0.01). However, here was no significant difference of enhanced degree between pulmonary inflammatory［（43.6±7.7)Hu］ and pulmonary carcinoma SPNs (P>0.10).But the time-density curve of malignant SPNs tend to achieve rapid enhancement- to-peak attenuation and eventually reached a plateau. Inflammatory SPNs showed gradual enhancement without obvious attenuation. The curve of tuberculosis was placid. Conclusion Single-location dynamic contrast enhanced CT is a non-invasive option and can provide quantitative information about blood flow patterns of SPNs.

Objective To establish a quick, specific and sensitive PCR method to detect Staphylococcus aureus (SA) in food and compare the PCR method with conventional approach. Methods The thermostable nuclease gene (nuc) of SA was amplificated specifically by PCR and 180 samples from different areas in Jilin Province were detected using PCR method and national standard approach respectively. Results The segments of nuc gene in 422 bp was found in the PCR products of all SA strains. 42 samples were positively tested by conventional method and 45 samples were positively tested by PCR method. There was no significant difference in positive rate between PCR method and national standard approach. Conclusion PCR method has higher specificity, sensitivity, quickness and convenience, which may provide a new approach to detect SA in food quickly.

Survivin是凋亡抑制蛋白(IAP)家族新成员，是迄今发现的作用最强的凋亡抑制因子，其结构在IAP家族中是非常独特的，含有单一的杆状病毒IAP重复序列(BIR)，而末端不含有环状结构。Survivin在人类多种肿瘤组织中过表达，而在分化成熟的组织中不表达。它通过干扰有丝分裂仿锤体的形成而调节周期G₂/M，通过抑制caspase-3和caspase-7的活性而抑制细胞凋亡。在肿瘤的形成中，Survivin表达与细胞凋亡呈负相关，而与细胞增殖和血管生成呈正相关。Survivin通过抑制细胞凋亡使癌症患者预后差且生存率降低。因而Survivin有望成为肿瘤诊断和治疗的新靶点。

非甾体抗炎药(NSAIDs)一直是世界上处方量最大的药物之一，广泛地用于治疗各种急性和慢性炎症。然而NSAIDs又是副作用发生率最高、最严重的药物之一，长期大量服用会诱发严重的消化道溃疡及出血，甚至会危及患者的生命。20世纪70年代，人们发现NSAIDs是通过抑制环氧合酶(cyclooxygenase,COX)，进而抑制前列腺素的生物合成而发挥抗炎作用。现在已经知道COX有两种同工酶，即COX-1和COX-2，选择性抑制COX-2不但能产生强大的抗炎作用，还可消除传统的非选择性NSAIDs由于抑制COX-1而诱发的毒副作用，因此COX-2是设计新型非甾体抗炎药的靶酶，其中吡唑衍生物类COX-2抑制剂的研究一直受到各国医药工作者的瞩目，目前主要开发了1，5-二芳基吡唑类、1，3-二芳基吡唑类及稠杂环吡唑类等三类衍生物。本文作者对上述三类抑制剂的结构改造及构效关系的研究进展进行综述。