Objective To overexpress human acidic fibroblast growth factor (aFGF) in Escherichia coli (E.coli) and study the biological activities of recombinant aFGF. Methods ①The codon of aFGF was altered by amplifying aFGF DNA and introducing point mutation; ②Human aFGF was recombined with pET-28a and expressed in E.coli; ③Recombinant protein was purified and the biological activities were studied. Results The projected point mutation was introduced into aFGF DNA fragment and the first 50 bp after ATG start codon of aFGF were altered to the partiality codon of E.coli by PCR, and the original sequence of amino acid was kept. So the overexpression of recombinant aFGF in E.coli was successfully accomplished. The expression volume of soluble aFGF in E.coli was greatly increased by lowering culture temperature. Recombinant aFGF protein had biological activities of nature aFGF. Conclusion By altering the bases of aFGF DNA, the expression volume of aFGF in E.coli is increased greatly, and the recombinant protein has nature biological activities.

Objective To study the roles of forkhead box C2 (Fox C2) in cardiovascular development. Methods Fox C2-deficient mice were reproduced by targeting mutation and the developmental anomaly in heart was analyzed. Results Of 98 newborns of mice, only 15 ones were homozygotes for Fox C2 mutation. Left and right ventricles of heart were communicated by a tiny crack on ventricular septa in these homozygote animals. In situ hybridization analysis on 10.5 days embryos showed that Fox C2 mRNA expressed at endocardium of ventricle. Conclusion Targeting mutation of Fox C2 gene can result in ventricular septal defect.

Objective To observe the histological changes of nerve repaired by CO₂ laser welding and silk suture and investigate the advantages of laser welding. Methods Thirty Wistar rats were divided into 2 groups randomly. The sciatic nerves of right legs were cut in all rats, then in one group the nerves were repaired by laser welding, in the other group the nerves were repaired by silk suture as control. The histological changes of nerves were observed in both groups at 7, 14, and 21 days after operation. Results Compared with silk suture, laser welding had the advantages of fast healing and less inflammatory reaction. Conclusion Laser welding can improve the quality of nerve repairing, it is beneficial for the function recovery of nerve, therefore it is a ideal nerve repairing technique.

Objective To study the effects of carbonic anhydrase Ⅱ produced by osteoclasts on bone resorption process. Methods The osteoclasts were isolated from long bones of one-day-old SD rats and seeded in ivory bones slices. Carbonic anhydrase Ⅱ inhibitor-acetazolamide of different concentrations were added to culture media. Changes of the bone resorption ability of osteoclasts were observed in 3rd day and 9th day to confirm the effects of carbonic anhydrase Ⅱ on osteoclastic bone resorption. Results Both the pit numbers and the pit areas were significantly inhibited by acetazolamide at the concentration of 10^-6　mol•L^-1, while 10^-8 mol•L^-1 acetazolamide inhibited mainly the pit areas. Conclusion Carbonic anhydrase Ⅱ can play a major role in osteoclast differentiation, maturity and activation by effecting the steady state intracellular pH. Thus, it may affect the bone resorption.

Objective To study the changes of peroxidase (POD) activities in the key organs in adult rats with diabetes. Methods Thirsty Wistar rats for 90 days were divided into three groups：control group (C), diabetes group treated with insulin (ID) and diabetes group (D), 10 rats in each group.The POD activities were determined by using guaiacol colorimetric method. Results The activities of POD in the hearts, sleeps,　and lungs in group D and group ID were decreased obviously (P＜0.05, P＜0.01), compared with control group; the POD activities in the hearts and lungs in group D were apparently lower than those of group ID (P＜0.05, P＜0.01). Conclusion The POD activities are decreased in rats with diabetes and insulin can partly recover POD activities in the key organs.

Objective To clone the sequence of the cDNA of mouse endostatin (mEndostatin) coding area and construct an expression vector containing Egr-1 promoter, IFNγ and endostatin genes. Methods Full length mEndostatin was obtained with the technique of RT-PCR and using mouse hepatocyte mRNA as template, pMD18T- mEndostatin was sequenced automatically and an expression vector containing Egr-1 promoter, IFNγ, and endostatin genes was constructed with gene recombinant technique. Results It was proved that the cloned mEndostatin cDNA to be completely identical with that reported in the literature and the recombinant plasmid containing Egr-1 promoter, IFNγ, and endostatin genes was constructed successfully. Conclusion mEndostatin cDNA was cloned and the expression vector pEgr-IFNγ-mEndostatin was constructed successfully.

Objective\ To study the effects of different dose Changle on enzymatic activity of rat liver. Methods\ 120 healthy Wistar rats were divided into three experimental groups of different doses (2.0,5.0,20.0　mg•kg^-1 Changle) and the control group were given physiological saline，one time a day respectively, and lasted for 3 months. The changes of SDHase，G-6-Pase, and Ca2+- ATPase in rat livers were observed by histochemical technique. Results\ Comparing with the control group,the levels of SDHase， G-6-Pase, and Ca2+- ATPase in hepatocytes in the group of 20.0 mg•kg^-1 were reduced obviously. Conclusion\ Changle can have an effect on rat hepatic enzymatic activity with the dose of 20.0　 mg•kg^-1.

Objective The U₁ small nuclear RNA(snRNA) has been exploited as a vector to construct U₁-chimeric ribozyme (U₁-Rz). Methods The DNA fragment of U₁-Rz was obtained by PCR on the pBSIISK+ U₁ containing the entire wide type human U₁ sequence. One of the primers was utilized containing the hammer head ribozyme gene sequence including its active core and blanking sequences, which targeted HCV core region. Twice subcloning were performed subsequently to construct the chimeric. Results The sequencing result showed that the third stem-loop of U₁ was just replaced by the ribozyme sequences. Conclusion U₁ snRNA chimeric ribozyme can be constructed by gene cloning strategy in vitro.

Objective To investigate anti-inflammatory effect of Gutongling ointment. Methods Fourty animals were divided into control and three different concentration (20%,40% and 80%) groups of Gutongling ointment. Anti-inflammatory effect of Gutongling ointment was carried through inflammatory models of auris swell induced by dimethylbenzene in mice, of foot swell induced by egg white and carageen glue in rats, of gasbag swell induced by croton oil in rats and penetration of capillary vessel. Results Gutongling ointment (40% and 80%,local use for three days) had significant inhibitory action on auris swell induced by dimethylbenzene in mice, foot swell induced by egg white and carageen glue, gasbag swell induced by croton oil and penetration of capillary vessel in rats. Conclusion Gutongling ointment has significant inhibitory effects on many experimental inflammatory models.

Objective The latency and auditory threshold of the cochlear action potential N₁ ［AP(N₁)］ were observed systematically after infrasound exposure　(125 dB) in guinea pigs. Methods The electrode was inserted into Fallopian′s cannal of guinea pigs for a long time during the study. And the AP(N₁) was compared with normal hearing guinea pigs.　 Results The auditory threshold was increased in different levels after 125 dB SPL infrasound exposure,and the hearing loss recovered to normal in 6-9 days. The recovery of latency was earlier than that of auditory thershold. Conclusion The temporary threshold shifting (TTS) of the auditory system may be caused after 125 dB SPL infrasound exposure or cochlear function is damaged temporarily.

Objective To study the expression of microvessel density (MVD), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF) in the rat model with Walker-256 hepatoma after chemoembolization. Methods Sixty rats with Walker-256 hepatoma were divided into 3 groups randomly at the 14th day after the tumors were implanted. Lipiodol 0.5 mL (TAIE group), lipiodol 0.5 mL plus 5-Fu 20 mg•kg^-1 (TACE group) and routine saline 0.5 mL (control group) were injected in different groups via hepatic artery to embolize tumor of rats. After 7 days of treatment, the expressions of MVD, VEGF, and bFGF in tumor tissues were examined with immunohistochemical technique. The correlation of VEGF expression intensity and MVD was analyzed. Results There was no significant difference of MVD between any two of the three groups (P>0.05). The positive rate and staining intensity of VEGF were significant higher in TAIE group and TACE group than those in control group (P<0.05, P<0.01), but there were no significant difference between TAIE and TACE group(P>0.05). The staining intensity of VEGF was significantly correlative to the MVD in tumor tissue (r=0.451，P<0.01). There were no significant differences of the positive rate and staining intensity of bFGF between any two of the three groups (P>0.05). Conclusion Survival tumor tissue was supplied with rich vascularity after TACE. The expression of VEGF was enhanded after TACE. VEGF and MVD may play an important role in reestablishment of bood supply for the tumor after TACE.

Objective To investigate the immunoisolation effect and biocompatibility of sodium alginate/chitosan. Methods The alginate-chitosan-alginate (ACA) was used as the microcapsule.Encapsulated PC12 cells，PC12 cells and empty microcapsule were transplanted respectively into the striatum of the Parkinsonian rats.The behavioral changes were tested by apomorphine.The morphological alterations were observed by striatal HE stain. The retrieved encapsulated PC12 cells were recultured and their activities were tested by MTT and Trypan blue test. Results The encapsulated PC12 cells could improve the rotational behavior of Parkinson′s disease (PD) model rats and there was a significant difference (P<0.05) comparing with pretransplantation group and empty capsule transplantation group. There was no disruption and no tumor formation in this group, and the inflammatory reaction around the capsule was slight. Although the unencapsulated PC12 cells could also improve the rotational behavior,the symptom improvement lasted only two months.In addition,several of the grafts developed into mortal tumor. The well growth and the good biological activities of the retrieved PC12 cells were proved using the MTT and Trypan blue test. Conclusion Sodium alginte/chitosan has a good biocompatibility. ACA microcapsule can both isolate the host immune system effectively and prevent the hypersegmentation of the cells inside the capsule.

Objective To study the effect of rotating magnetic field on metabolism of monoaminergic neurotransmitters in mice. Methods Healthy aging mice of Kunming species were divided into two groups randomly: experimental group and control group.The heads of experimental mice were treated with a rotating magnetic field at 2 000 r•min^-1 and 0.09T for 30 min per day,lasted 15 days;Mice in control group were treated at the same way after the rotating magnet was taken out. Monoaminergic neurotransmitters were determined by chemical fluorescence technique 15 days later. Results Compared with control group,the concentration of NE,DA,5-HT and 5-HIAA increased apparently (P<0.05). Conclusion The rotating magnetic field in a certain intensity and lasting a certain time can increase the content of monoaminergic neurotransmitters in body at a certain extent and it can also inhibit the caducity.

Objective To study the effect and significance of bactericidal/permeability increasing protein (BPI) on the TNF-α and IL-6 mRNA expressions and protein levels in the middle ear mucosa of the rat models of otitis media with effusion (OME). Methods Rats were subjected to OME by injecting lipopolysaccharide (LPS) into middle ear cavity, and animals were divided into LPS group and BPI group. TNF-α and IL-6 mRNA expressions and their protein levels in middle ear mucosa were measured at various intervals with ELISA and RT-PCR. Results IL-6 mRNA expressions and protein levels peaked at the 1st and 2nd day in LPS group and BPI group. However, IL-6 mRNA in BPI group as well as protein levels were significantly reduced compared with LPS group（P<0.05）.There were no expressions of IL-6 mRNA and protein in the LPS group and BPI can group after 7 days. However, TNF-α mRNA expressions and protein levels persisted in LPS group and BPI group. TNF-α mRNA in BPI group as well as protein levels were significantly reduced compared with LPS group（P<0.05）. Conclusion IL-6 and TNF-α have an important role in the development of OME; BPI can prevent the expression and secretion of cytokines, reduce inflammatory activities of cytokines; BPI is a potential drug for preventing the occurrence of chronic OME.

Objective To investigate the therapy effects of the antisense oligonucleotides of telomerase on the BIU87 cell derived from bladder cancer. Methods Telomerase activities in 25 tissue specimens of bladder cancer and 4 normal ones were detected by TRAP-AgNO₃ stain.The phosphorothioate antisense oligonucleotides (PS-ASON) of differential doses with sequence TTAGGGTTAGACAA targeted to telomerase RNA template (as experiment group A,B, and C), PS-random primer with 13 nucleotides (as control group,D),and culture medium only (as empty control group,E) were incubated with the BIU87 cell lines. The number of cells were counted from 1 to 30 days, The apoptotic rate was assayed by flow cytometry. Apoptosis was detected by DNA ladder electrophoresis. The cell form was observed with light and electron microscope. Results Of 25 cases of bladder cancer, the activities of telomerases were expressed in 24 cases (96%), there was no expression in 4 normal tissues. ASON in experiment group reduced the number of cells compared with group D and E. The transparent apoptotic cusp at flow cytometry and revealed ladder electrophsis zone were presented in experiment groups at 2nd, 8th day and 8 days later. Degeneration, aging, necrosis, and apoptosis occurred under the microscope. The efficacy depended on the doses of ASON (P<0.01) and it was of sequence-selective specifity. Conclusion Inhibition of BIU87 cell proliferation, aging,necrosis,and apoptosis induced by ASON targeted hTR may have potential significance in tumor therapy.

Objective To establish a cell line Hela-WAF1 of efficient and stable expression on p21WAF1/CIP1. Methods Recombined vector pcDNA3-WAF1 and vector pcDNA3 were transferred to Hela cell line by lipofectin, respectively. These two cell lines were selected by G418, and the selected cell lines were subcultured. Hela cell line was enclosed as control group. The expression of p21WAF1/CIP1 was detected by immunofluorescence. Results After G418 selecting, Hela cell line in control group was died, and the cell lines transferring pcDNA3 and recombined pcDNA3-WAF1 were subcultured over 30 generations. The p21WAF1/CIP1 expression in Hela-pcDNA3-WAF1 was 92.83%. The results showed that recombined gene of WAF1 was more efficient expression, compared with other two groups (F=17 218.22,P<0.001); There was no significant difference in every generation (F=1.562,P>0.05). Conclusion The cell line Hela-pcDNA2-WAF1 of efficient and stable expressing p21WAF1/CIP1 is established successfully.

Objective\ To determine the expression and distribution of aquaporin 1-9（AQP1-9）in male reproductive tract of mouse. Methods The expressions of AQP1-9 in various organs of male reproductive tract of mouse was determined using RT-PCR. Results The expression of AQP3，5，7，8，9 were present in mouse testis. AQP3，6，8 expression were found in capita epididymis and AQP3，5，6，7，8 in caudae epididymis. In vas deferens， the AQPs except for AQP8，9 were detected. Conclusions The different localization of AQP1-9 expression in male reproductive system of mouse shows that they may play an important role in physiological function of male reproductive system，especially for the maturation of sperm in different segments of male reproductive tract of mouse.

Objective\ To observe the effect of Acanthopanax senticosus saponins (ASS) on total cholesterol (TC), lipoprotein cholesterol metabolism and antioxidative activity in experimental hyperlipidemia rats. Methods The TC, lipoprotein cholesterol and lipid peroxidation (LPO) contents, prostacycline (PGI₂) and thromboxane (TXA₂) levels, superoxide dismutase (SOD) activity and blood viscosity were measured respectively in hyperlipidemia rats which had been given ASS 25, 50 and 100 mg•kg^-1•d^-1 ip, respectively, for twenty-one days. In addition, fat accumulation in liver was observed. Results The triglyceride (TG), TC, low density lipoprotein cholesterol (LDL-c) in serum, TXA₂ in plasma, LPO in serum and liver and blood viscosity were decreased significantly, and high density lipoprotein cholesterol (HDL-c) in serum, PGI₂ in plasma and SOD in serum and liver were significantly increased by given ASS (50 and 100 mg•kg^-1•d^-1) in experimental hyperlipidemia rats. Moreover, ASS could decrease ratioes of TC/HDL-c, LDL-c/HDL-c, increase the ratio of PGI₂/TXA₂ and inhibit fat accumulation in liver. Conclusion ASS can inhibit arteriosclerosis by improving cholesterol and lipoprotein cholesterol metabolism, suppressing LPO,and increasing the activity of antioxidase.

Objective To study the effect of anti-cerebral ischemia of gross saponin from tribulus terrestris (GSTT). Methods A focal cerebral ischemia model was set up through blocking one-side medium cerebral artery, the effects of GSTT on infarction area and behavior scoring (neuological deficits) were observed; blood stasis model was built in rats, the effects of GSTT on platelet aggregation rate and clotting time were observed. Results GSTT with dose of 10.4, 20.8, and 31.2 mg•kg^-1 could significantly decrase the infarction area and improve behavior score(P<0.05, P<0.01); GSTT with dose of 10.4, 20.8, and 31.2 mg•kg^-1 could remarkably decrase the platelets aggregation rate (P<0.05), and markedly prolong the clotting time in blood stasis rats. Conclusion GSTT has the effect of anti-cerebral ischemia and its mechanism may relate to lowering platelets aggregation rate and prolonging clotting time.

Objective To elucidate the effects of Diemailing injection (DMLI) on organ blood flow and vascular resistance in anesthetized dogs. Methods Cerebral blood flow，cerebral vascular resistance，peripheral blood flow,peripheral vascular resistance,blood pressure, and heart rate (HR) were measured using the MF-27 electromagnetic flowmeter. Results DMLI(4 and 8 mL•kg^-1 iv drip) increased cerebral blood flow and decreased cerebrovascular resistance within 180 min，its effect was more obvious than Mailuoning injection (MLNI).DMLI also elevated peripheral blood flow and lowered peripheral vascular resistance,its effect was less than MLNI.HR was slowed down only at 60 min whereas at other time not.DMLI obviously reduced diastolic pressure but exerted no effect on systolic pressure. Conclusion Protective effect of DMLI on experimental cerebral ischemia may be related to improving cerebral circulation and increasing cerebral blood supplyment.

Objective\ To investigate effects of Acanthopanax senticosus saponins（ASS） on ventricular remodeling in rats with acute myocardial infarction. Methods The myocardial infarct model was induced by left anterior descending coronary occulusion in rats. After 4 weeks of treatment by ASS, hemodynamic changes, biochemical analysis and morphological parameters were determined in ventricular remodeling rats. Results The maximum left ventricular presure rising and dropping rates (±dp/dtmax) and their left ventricular systolic pressure corrected values(±dp/dtmax/LVSP) were increased significantly. The left ventricular end diastolic pressure (LVEDP), the left ventricular volume (LVV), the left ventricular long and short axis(LVLA and LVSA), the left ventricular absolute and relative weight (LVAW and LVRW) were reduced significantly, but there were no significant influences on the right ventricular absolute and relative weight (RVAW and RVRW), the heart rate (HR), the left ventricular systolic pressure (LVSP), mean artery pressure (MAP) and body weight (BW). In addition, the serum lipid peroxidation (LPO) content, myocardial angiotensin Ⅱ (AngⅡ) and epinephrine (E) contents were declined, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were increased markedly. Conclusion ASS can prevent and treat ventricular remodeling after myocardial infarction in rats efficiently.

Objective To study the effects and mechanisms of Sanshen Ziwei Yin(SZY) on D cells and G cells of gastric mucosa in rats with chronic atrophic gastritis (CAG). Methods Rat models with CAG were established with synthesis method (active immunity,cold and cool gall and hot water irrigation). High and low-dose of SZY and Weimeisu (WMS,as control) were used for 50 days constantly. The pathological changes of gastric mucosa,morphological and number changes were observed before and after treatment. Results The number of G cells and D cells of rats with CAG decreased significantly (P<0.01), and degranulation presented in both two kinds of cells. The decrease of G/D ratio indicated that G cells descented more fast than D cells, that is to say, G cells were more sensitive to CAG. After treatment,the number of D cells increased obviously in treatment group, and apporached to normal levels at last,there was significant difference compared with WMS group. Conclusion SZY may have therapeutic effects on CAG through improving the inflammatory reaction of gastric mucosa to promote the regeneration of gland, endocrine and paracrine cells,and improving the regulation effects of gastrointestinal hormones on gastric function.

Objective \ To investigate the extraction of type I collagen and the preparation of collagen membrane. Methods\ Fresh adult bovine tendon was dissolved with acetic acid and enzyme-digestion for type I collagen. Then they were dialysed and purified for refined liquid collagen, which was cross-linked with glutaraldehyde. Results\ The maximum absorbance of the purified collagen appeared at 230 nm， with correspondence to type I collagen，two close bands on SDS-PAGE (mol.wt 98 000) suggested the purified product was type I collagen. The extracted collagen was white, with no smell. The collagen membrane was white, sponge-like and elastic. SEM showed structure of holes and nets of collagen membrane, the diameter of which was 15-50 μm. Conclusion:The method of acetic acid dissolution and enzyme digestion is a simple and ideal one for extracting bovine tendon collagen. The type I collagen is much and highly purified with low antigen.

Objective\ To study the osteogenic property of the induced rabbit bone messenchymal stem cells(MSC) in nude rats. Methods MSC were obtained by using gradient centrifuge method. The cells had been cultured in mineralized medium for 1 week, then the ALP activity was observed. The induced MSC/antigen-extracted bovine cancellous bone were implanted in the nude mice subcutaneously. At 3rd day after implantation, the implants underwent the histological analysis. Results ALP activity was higher and the cells displayed fibroblast-like morphology, revealed positive staining with alkaline phosphatase method in the induced medium. At 3rd day after implantation, histological analysis revealed that the induced cells were adhered at the surface of the bone trabeculase and growed well. Exogenous fibro-tissue, blood vessel and free induced cells were invisible. Conclusion Rabbit MSC could differentiate into osteoblast and proliferate induced by the mineral medium. The induced MSC/antigen-extracted bovine cancellous bone could survive in the nude rat.

Objective To study the expression of heme oxygenase-1 (HO-1) induced by doxorubicin in liver,spleen, and kidney and the effect of doxorubicin on liver function. Methods Male Wistar rats were randomly divided into control, low-dose (1 mg•kg^-1) and high-dose(10 mg•kg^-1) group. The expressions of HO-1 in liver were detected by Western blot in the two doxorubicin groups 6,12,18,24, and 48 hours later after the injection. The expressions of HO-1 in liver,spleen and kidney were also detected by immunohistochemistry 48 hours later after the injection and at the same time the indicator of liver function test, serum TNF-α and ET-1 were also determined in the three groups. Results The expression of HO-1 in liver could be detected by Western blot 6 hours later after the injection in the two doxorubicin groups and the expression increased continuously until 24 hours later. The expression of HO-1 was also detected in liver,spleen and kidney by immunohistochemistry 48 hours later after the injection of doxorubicin. The indicator of liver function test,serum TNF-α and ET-1 were much higher in the high-dose doxorubicin group than those in the control group and low-dose doxorubicin group (P<0.05), there was no significant difference between the control and low-dose doxorubicin groups (P>0.05). Conclusion Doxorubicin can induce the expression of HO-1 in liver,spleen and kidney, low-dose doxorubicin may do no harm to liver.

Objective To investigate the effect of low dose radiation(LDR) with different dose of X-rays on P53 and Bcl-2 protein expression in spermatogenic cells of male Kunming mouse testis. Methods The relationships between time-effect and dose-effect of P53 and Bcl-2 protein expression positive rate in spermatogenic cells of mouse testis after LDR with different dose of X-rays were studied with immunohistochemical technique (SABC). Results P53 and Bcl-2 protein expressed in spermatogonia and spermatocytes in varying degrees, the positive rate of spermatogonia was obviously superior to that of spermatocytes. With the increase of irradiation dose, the expression of P53 protein showed a increasing tendency, however, the P53 protein expression of spermatozoa scarcely occurred after LDR. Bcl-2 protein was primarily expressed in spermatozoa. With the increase of irradiation dose, the positive rate of Bcl-2 protein expression showed a downregulated tendency. However, the Bcl-2 protein expression of spermatogonia and spermatocytes scarcely occurred after LDR. Conclusion The expressions of P53 and Bcl-2 may have regular changes in mouse testis induced by LDR, which may provide a experimental evidence for the mechanism study of spermatogonic cell apoptosis induced selectively by ionizing radiation.

Objective To investigate the immunopathological change of the mice immunized with P-glycoprotein gene vaccine. Methods The mice were twicely immunized with either P-glycoprotein gene vaccine at 20-100 μg or the control vector at a 9 days interval. The murine sera were collected to analyze antibody (Ab) titer. Tissue samples of the heart,liver and kidney of the mice were examined by HE staining. Results The titer of Pgp-specific IgG Ab reached as high as 1∶4 000. Pathological damage was found in liver of the immunized mice, indicated by infiltration of mononuclear cells and scattered necrosis of the hepatocytes. Conclusion The P-glycoprotein gene vaccine can induce the experimental hepatitis in mice, which may be used as an animal model for human autoimmune hepatitis.

Objective To research the characteristics of various ion channels of habenuler neurons. Methods The characteristics of various ion channels of habenuler neurons on the acute isolated habenuler neurons of rats by using patch-clamp technique. Results Na⁺ current and two kinds of outward K⁺ currents were respectively recorded at a hoding potential of 10 mV steps. Conclusion The delayed rectifier K⁺ channels mainly distribute in the lateral habenula ,while rapid K⁺ channels in medial habenular. This result is related to the difference of structure and function between lateral and medial habenular.

Objective To investigate the clinical significance of the changes of tumor necrosis factor α(TNF-α) and interleukin-8 (IL-8) in rabbits after extracapsular extraction (ECCE) and posterior chamber intraocular lens (PCIOL) implantation. Methods The level of TNF-α and IL-8 from the samples of 4 mL venous blood and 300 μL aquous humor were determined by radioimmunoassy in 24 rabbits with intraocular lens (IOL) implantation and 20 normal rabbits at the same time with the operation group. Results The levels of TNF-α and IL-8 in serum and aqueous humour after operation were increased in 24 rabbits and which related to postoperative recovery. Conclusion The levels of TNF-α and IL-8 in rabbits after ECCE and PCIOL implantation may be indexes to judge the postoperative recovery and to predict progress.

Objective To study the influence of hypericin on the proliferation and apoptosis of cultured human prostate cancer cells. Methods Growth inhibition test and flow cytometry were used to examine the changes of proliferation, cell cycle and apoptosis. Results Hypericin of different concentrations could significantly inhibit the proliferation and induce the apoptosis of human prostate cancer cells cultured in vitro. When the concentrations were 0.5,1.0,2.0 mg•L^-1,the inhibitory rates were 21%,50%, and 91%,respectively. S phase was blocked and didn′t change into M₂ phase. Conclusion Hypericin can inhibit the proliferation and cell cycle of cultured human prostate cancer cell and induce its apoptosis.

Objective To study the relationship between the resistance R plasmid and both pili and adherence of Pseudomonas aeruginosa strain,PA36. Methods Agarose gel electrophoresis of plasmid DNA, adherence test, and extract of plasmid were used. Electron microscope photos were prepared and observed. Results The wild type strain of PA36 contained a plasmid (3.3×10⁶), heavy pili were found on the surface of PA36 under electron microscope, crowed bacteria cells adhered to dropped urethra-epithelial cells under light microscope. After the plasmid of PA36 was cured by SDS, both the resistance and pili almost completely disappeared and the capability of adherence weakened sharply in the meantime. Conclusion The results suggested that gene encoding ordinary pili of wild type PA36 may be located in the R plasmid, which is responsible for the adherence of PA36. Thus, the R plasmid in PA36 also names adherence plasmid. The gene also is relates to the pili and the capability of adherence.

Objective To study the relationship between apoptosis and cell cycle with Hep-2 cell apoptosis induced by high temperature. Methods To examine Hep-2 cell proliferation inhibitory rate,apoptosis and different phase of cell cycle with MTT colorimetry and flow cytometry. Results The difference of Hep-2 apoptosis rate between heating group and control group was obviously significant;The different of Hep-2 proliferation inhibitory rate between heating group and control group with MTT colorimetry was obviously significant (P<0.01). Conclusion High temperature can increase Hep-2 cell apoptosis and inhibit the cell cycle progress of Hep-2 from G₁ stage to S stage.

Objective To detect EB virus in peripheral blood of patients with nasopharyngeal carcinoma by ramification amplification method (RAM). Methods RAM was used to detect EB virus with the synthetic target gene sequence of EBER-1. EB viruses in some cases of patients with nasopharyngeal carcinoma were detected by RAM and PCR, respectively, and the result of RAM was compared with that of PCR. Raji cells and NB4 cells were used as positive control and negative control respectively. Results RAM could detect 10 molecular target gene sequence of the samples, 28 cases were positive in 32 patients with nasopharyngeal carcinoma and the positive rate was 87.5%, The result was identical with that of PCR. Conclusion RAM is sensitive and convenient, and also easy to perform.

Objective To study the effect of CB₄V infection on human embryonic islet cell. Methods Human embryonic islet cell was infected by CB₄V in vitro, the morphological change of that was observed by electro microscope, and insulin releasing test by sugar stimulation was also proceeded. Results CB₄V could attack islet directly ,break the envelope of islet, invade β cell and proliferated in β cell. The quantity of insulin releasing stimulated by sugar increased 24 h after infection, then decreased gradually. The quantities of insulin releasing stimulated by hypoglycemia and hyperglycemia in infected group were (64.50±9.20)mU•L^-1 and (87.85±8.50) mU•L^-1,respectively,24 h later after infection, which showed significant difference compared with the control group (P<0.05). From 48 h to 6 d, the quantities of insulin releasing stimulated by hypoglycemia and hyperglycemia were much lower than that of control group (P<0.05，P<0.01).Conclusion CB₄V can infect human embryonic islet cell in vitro, and damage the function of islet β cell of secreting insulin.

Objective To study the micrometastasis in lymph nodes of the patients with gastric cancer and its clinical significances. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect CK18 mRNA expression in 298 lymph nodes from 35 patients with gastric cancer and 20 lymph nodes from control group. Results There was no CK18 mRNA expression in control group. In experimental group, the positive rates of CK18 mRNA expression detected with routine histology and RT-PCR were 33.2% (99/298) and 44.6% (133/298),respectively,there was significant difference (P<0.05). Of lymph nodes in which routine histology failed to find metaslasis, 17.1% lymph nodes were found the micrometastasis by CK18 mRNA RT-PCR. Conclusion CK18 mRNA RT-PCR is more sensitive than routine histology for detecting the micrometastasis in lymph nodes of gastric cancer, and has important clinical significances in evaluating clinical staging, predicting prognosis and directing postoperative treatment.

Objective To investigate the C-reactive protein（CRP）level and observe the efficacy of lipitor on the lipids and CRP in the patients with hyperlipidemia. Methods The serum CRP was measured in 42 patients with hyperlipidemia and 30 healthy subjects; The lipids and CRP were measured again in patients with hyperlipidemia after 8 weeks of lipitor treatment. Results The serum CRP was obviously higher in hyperlipidemia patients than that in the subjects(P<0.05); After 8 weeks lipitor treatment, TC,TG,LDL, and CRP were decreased significantly ( P<0.05 or P<0.01). Conclusion The inflammation marker-CRP is increased in the patients with hyperlipidemia; lipitor may not only largely mediate lipids but also decrease vascular inflammation reaction in patients with hyperlipidemia.

Objective To investigate the mechanism of spasticity in cerebral palsy（CP）. Methods Cerebrospinal fluid was obtained from spastic cerebral palsy group (CP, n=45), control group (children without central nervous system disenses, n=30). The concentrations of inhibitory amino acids (IAAs) and excitatory amino acids (EAAs) in cerebrospinal fluid were determined by dimethylamino-naphthalene-s-sulfonyl chloride with polyamine thin layer chromatography. Results The level of GABA was significantly lower than that of the control group　(P<0.001) whereas GA and Asp were significantly higher than that of the control group (P<0.001). The level of GA correlated positively with muscular tone. The level of Gly was not significantly different from that of the control group. Conclusion The increase of excitatory postsynaptic potential (EPSP),the decrease of presynaptic inhibition and postsynaptic inhibition and the formation of new synapses by sprouting of afferent axon terminals may play an important role in spasticity of cerebral palsy.

Objective To investigate the functional changes of vein endothelial cells (VECs) of the newborns of the women with severe pregnancy-induced hypertension syndrome (PIH). Methods The newborn VECs from 16 cases of severe PIH women and 17 cases of normal pregnancy women were cultured by pancreatin digestion, supernatant fluid was collected after 48 h, the levels of 6-keto-pGF1_α (terminal product of metabolism from ET-1,PGI₂)， NO-₂ and VWF (terminal product of metabolism from NO) were detected. Results The level of ET-1 in VECs of severe PIH group was increased,but compared with the control group,there was no significant difference (P>0.05). The levels of NO^-₂ and 6-keto-pGF1_α were decreased,compared with control group,there was significant difference (P<0.01), the concentration of VWF factor increased remarkbely (P<0.05). Conclusion The injure and functional change of VECs are present in newborns of severe PIH women.

Objective To observe the effects of complex pharmaceutics containing carnitine and eortcd on oxygen free radical (OFR) activity in acute coronary syndrome (ACS) patients. Methods Forty patients with ACS were divided into two groups randomly：carnitine and eortcd therapy group (A group, 20 cases) and routine therapy group (B group ,20 cases). 20 healthy blood donors were served as control (C group).Patients in A group were given carnitine and eortcd 10 mL ,bid p.o., twice a day for 30 days, and other administrations were the same as B group. The levels of serum SOD and MDA in A and B group before and after therapy were detected. Results The levels of serum SOD in A group before therapy and B group before and after therapy were lower than that in C group (P<0.01)，there was no diffence between A group after therapy and C group, as well as A and B group before therapy (P>0.05). After therapy, A group was higher than B group. The concentration of MDA in A and B groups were higher than that in C group (P<0.01).The level of serum SOD in A group increased significantly after therapy (P<0.05), there was no significant difference in B group before and after therapy (P<0.05); the MDA in A and B groups decreased markedly after therapy, compared with C group (P>0.01), before and after therapy, there was no significant difference between A and B group (P>0.05). The levels of serum MDA after therapy in A and B group were lower than before therapy (P<0.01), however, they were higher than C group. Conclusion Canitine and eortcd can alleviate the injury of OFR and has protective function on ischemic myocardium in ACS patients.

Objective To determine the relationship between the expression of tissue-inhibitors of metalloproteinases-1 (TIMP-1) and the prognosis of laryngeal carcinoma (LG). Methods TIMP-1 was analyzed using immunohistochemical technique in 45 cases of laryngeal squamous cell carcinoma. Results The level of TIMP-1 expression in laryngeal carcinoma was less than those in adjacent and normal tissues. TIMP-1 expression level decreased with the increase of the clinical stages. The group with high level TIMP-1 expression significantly related to the high survival rate.Conclusion Detecting the expression of TIMP-1 in the tissue of laryngeal carcinoma may become an useful indicator to predicate the probability of metastasis and prognosis of laryngeal carcinoma.

Objective To evaluate the efficacy and safety of a combination use of clobetasol propionate and retin-A cream in the treatment of psoriasis vulgaris. Methods A randomized controlled and double blind trial was used. 72 patients were divided into trial group（combination of clobetasol propionate and retin-A cream）,control group 1（Diwei cream）,and control group 2（Enfu cream）. The efficacy and safety were observed. Results The cure rate and efficacy rate were 58.3% (14/24) and 100.0% (24/24),respectively, for trial group whereas cure rate and efficacy rate were 41.7% (10/24) and 75.0% (18/24), respectively, for control group 1 and 16.7% (14/24) and 75.0% (18/24) for control group 2. The difference was significant between various groups (P<0.05). There were no local and general adverse effects of drugs in all groups. Conclusion A combination use of clobetasol propionate and retin-A cream is effective and safe in treatment of psoriasis vulgaris.

Objective To study the clinical significance of posteroanterior (PA) radiographs and cephalometries for diagnosis of facial asymmetry of adults. Methods PA radiographs in 16 cases of facial asymmetry patients (experiment group, 8 males and 8 females) and 16 normal adults with facical symmetry (control group, 8 males and 8 females) were taken and analyzed. The data was analyzed with Student T test. Results There were significant differences of DifManM-Y，DifMaxM-Y, DifGo-Y，Me-Y and LI-Y between experiment group and control group by PA radiographs and cephalometries (P<0.01,P<0.05). Conclusion PA radiographs and cephalometries have a specific significance of diagnosis for facial asymmetry in adults.

Objective To isolate endothelial progenitor cells from umbilical cord blood and observe their biologic behaviors. Methods The mononuclear cells were isolated from umbilical cord blood using Ficoll gradients, and then were cultured on fibronectin. The characteristics of the attached cells were got and they were subjected to immunocytochemical method to analyze the expression of von Willebrand factor (vWF). Results After the mononuclear cells were cultured for 3 days, a large amount of attached cells and some spindle-shaped cells were observed. The number of spindle-shaped cells increased until 7th day. The immunocytochemical method showed that the attached cells were positively stained for vWF. Conclusion The endothelial progenitor cells may be isolated from umbilical cord blood.