The endometrial cancer HEC-1B cells in logarithmic growth phase were divided into control group（DMSO）， positive control group（2.5 mg·L^－1cisplatin），low dose（10 μmol·L^－1）of oridonin group， medium dose （20 μmol·L^－1）of oridonin group and high dose （40 μmol·L^－1）of oridonin group. MTT assay was used to detect the inhibitory rates of proliferation of cells. Flow cytometry was used to determine the apoptotic rates of HEC-1B cells. Cell scratch test was used to detect the cell migration ability. Transwell chamber assay was used to detect the invasion ability of HEC-1B cells. Real-time fluorescence quantitative PCR（RT-qPCR） was used to detect the expression levels of lncRNA CCAT1 in the cells.

Compared with control group， the apoptotic rates of HEC-1B cells in positive control group， low，medium and high doses of oridonin groups were significantly increased （P<0.05），the scratch healing rates and the number of transmembrane cells were significantly reduced （P<0.05），and the expression levels of lncRNA CCAT1 in the HEC-1B cells were significantly decreased （P<0.05）. Compared with low dose of oridonin group， the apoptotic rates of HEC-1B cells in medium and high doses of oridonin groups were significantly increased （P<0.05），the scratch healing rates and the number of transmembrane cells were significantly reduced （P<0.05），and the expression levels of lncRNA CCAT1 in the HEC-1B cells were significantly decreased （P<0.05）. Compared with medium dose of oridonin group， the apoptotic rate of HEC-1B cells in high dose of oridonin group was significantly increased （P<0.05），the scratch healing rate and the number of transmembrane cells were significantly reduced （P<0.05），and the expression level of lncRNA CCAT1 in the HEC-1B cells was significantly decreased （P<0.05）.

Oridonin can inhibit the proliferation， migration，and invasion and promote the apoptosis of endometrial cancer HEC-1B cells， and its mechanism may be related to down-regulating the expression level of lncRNA CCAT1.