Abstract:

Objective To discuss the effect of paeonol on the proliferation， migration and chemokinereceptor4 （CXCR4）/signal transducer and activators of transcription3 （STAT3） pathway of the human osteosarcoma（OS ） MG-63 cells， and to clarify its possible mechanism. Methods The MG-63 cells were cultured in vitro and divided into control group （without drug intervention） and different concentrations of paeonol groups （given 10， 20， 40， 80， 160，and 320 mg·L^－1 paeonol， respectively）.The survival rates of the MG-63 cells in various groups were detected by CCK-8 method and the half inhibitory concentration （IC₅₀） value was selected as the drug concentration for subsequent experiment. The MG-63 cells were divided into control group（without drug treatment） and paeonol group（given 215.8 mg·L^－1 paeonal）.The expression levels of CXCR4， interleukin 6 （IL-6），and phosphorylated STAT3 （p-STAT3），and STAT3 proteins in the MG-63 cells in various groups were detected by Western blotting method.The MG-63 cells were divided into control group， paeonol group， paeonol+empty group （given 215.8 mg·L^－1 paeonol+empty plasmid）， and paeonol+overexpression group （given 215.8 mg·L^－1 paeonol+CXCR4 over-expression plasmid）. The scratch healing rates of the MG-63 cells in various groups were detected by cell scratch experiment， the clone formation rates the MG-63 cells in various groups were detected by cloning formation experiment， and the expression levels of CXCR4， IL-6， p-STAT3， and STAT3 proteins in the MG-63 cells in various groups were detected by Western blotting method. Results Compared with control group， the survival rates of the MG-63 cells treated in 40， 80， 160， and 320 mg·L^－1 paeonol groups were decreased （P<0.05）， and the IC₅₀ value of paeonol was 215.8 mg·L^－1. Compared with control group， the expression levels of CXCR4， IL-6， and p-STAT3 proteins in the MG-63 cells in paeonol group were decreased （P<0.05）. Compared with control group， the scratch healing rates and clone formation rates of the MG-63 cells in paeonol group and paeonol+empty group were decreased （P<0.05）， and the expression levels of CXCR4， IL-6，and p-STAT3 proteins in the MG-63 cells were decreased （P<0.05）； compared with paeonol group，the scratch healing rate， clone formation rate， expression levels of CXCR4， IL-6， p-STAT3，and STAT3 proteins in the MG-63 cells in paeonol+empty group had no significant differences（P>0.05）； compared with paeonol group， the scratch healing rate and clone formation rate of the MG-63 cells in paeonol+overexpression group were increased （P<0.05）， and the expression levels of CXCR4， IL-6 and p-STAT3 proteins were increased （P<0.05）. Conclusion Paeonol can inhibit the proliferation， migration，and clonogenic ability of the MG-63 cells， and its mechanism may be related to the regulation of CXCR4/STAT3 signal pathway.

Key words: Paeonol, Human osteosarcoma cells, Cell proliferation, Chemokinereceptor 4, Signal transducer and activators of transcription 3