Abstract:

Objective To discuss the inhibitory effect of schisandrin B （SchB） on the migration and invasion of the pancreatic cancer Pan02 cells， and to clarify its mechanism. Methods The pancreatic cancer Pan02 cells were treated with different concentrations of SchB （0， 0.78， 1.56， 3.12， 6.25， 12.50， and 25.00 mg·L^-1） for 24， 48， and 72 h. CCK-8 method was used to detect the survival rates of the cells in various groups， and the concentration of SchB for the subsequent experiments was confirmed. The Pan02 cells were divided into control group， 2.5 mg·L^-1 SchB group， 5.0 mg·L^-1 SchB group， and 10.0 mg·L^-1 SchB group. Wound healing assay was used to detect the wound healing rates of the Pan02 cells in various groups； Transwell chamber assay was used to detect the numbers of migration and invasion Pan02 cells in various groups； Western blotting method was used to detect the expression levels of Vimentin and N-cadherin proteins in the Pan02 cells in various groups.The mouse models of subcutameous transplanted tumor of pancreatic cancer cells were established.Ten successfully modeling mice were randomly divided into control group and SchB group （n=5）. After 28 d of treatment， the weights of tumor of the mice were determined； immunohistochemistry method was used to detect the expressions of Vimentin and N-cadherin proteins in tumor tissue of the mice in various groups. Results The CCK-8 results showed that compared with control group， the survival rates of the pancreatic cancer Pan02 cells in different concentrations of SchB groups were decreased （P<0.05 or P<0.01）. The wound healing results showed that compared with control group， the wound healing rates of the cells in 2.5， 5.0， and 10.0 mg·L^-1 SchB groups were decreased （P<0.05 or P<0.01）. The Transwell chamber results showed that compared with control group， the numbers of migration and invasion Pan02 cells in 2.5， 5.0， and 10.0 mg·L^-1 SchB groups were decreased （P<0.05 or P<0.01）. The Western blotting results showed that compared with control group， the expression levels of Vimentin and N-cadherin proteins in the Pan02 cells in 2.5， 5.0， and 10.0 mg·L^-1 SchB groups were decreased （P<0.05 or P<0.01）. Compared with control group， the tumor volume and weight of the mice in SchB group were significantly decreased（P<0.01）. The immunohistochemistry results showed that compared with control group， the positive expression rates of Vimentin and N-cadherin proteins in tumor tissue of the mice in SchB group were significantly decreased （P<0.01）. Conclusion SchB can inhibit the proliferation， migration， and invasion of the pancreatic cancer Pan02 cells， and its mechanism is related to the reduction of expressions of Vimentin and N-cadherin proteins.

Key words: Schisandrin B, Pancreas neoplasms, Cell migration, Cell invasion, N-cadherin, Vimentin