人工回灌过程中地下水微生物群落变化

doi:10.13278/j.cnki.jjuese.201502206

摘要/Abstract

摘要：

人工回灌过程中,回灌水的注入使目的含水层地下水环境发生变化,微生物条件也会随之改变,从而影响地下水环境质量及水文地球化学作用。以上海市某人工回灌试验场为例,在分析人工回灌过程中水化学演化特点的基础上,应用DGGE技术对场地回灌过程中地下水中的微生物群落结构变化进行研究,为评价人工回灌对地下水水质安全的影响提供科学的理论依据。结果表明:人工回灌作用使目的含水层地下水中的Eh值及DO质量浓度升高,分别由64.0 mV、1.12 mg/L升至534.4 mV、1.44 mg/L;同一位置处微生物群落结构与原始地下水状态的相似性随时间降低;同一时刻距离回灌井越远的监测井的微生物群落结构越接近于原始地下水状态。随着回灌的进行,目的含水层地下水中优势菌属(种)共有7种,其中Rubrivivax gelatinosus和Candidatus Accumulibacter phosphatis clade IIA str.的反硝化能力以及Rhodoferax ferrireducens对Fe³⁺的还原能力,对地下水水化学组分产生影响。

关键词: DGGE, 人工回灌, 地下水, 微生物群落

Abstract:

The injection of the recharge water changes the groundwater environment and the microenvironment of the objective aquifer, and influences the groundwater environment quality and the hydrogeochemical process during the artificial recharging process. We used the denaturing gradient gel electrophoresis (DGGE) technology to study the change of the groundwater microbial community based on the analysis of the hydrochemistry characteristic and provideda scientific basis for evaluating the effect of groundwater quality by the artificial recharge. The results show that the artificial recharge makes the Eh and DO value of the objective aquifer groundwater increase from 64.0 mV and 1.12 mg/L to 534.4 mV and 1.44 mg/L, the microbial community similarity between the groundwater of the monitoring well and the original groundwater decreases over time but increases with distance. Seven types of microorganisms are identified in the groundwater samples of the objective aquifer, the denitrification of Rubrivivax gelatinosus and Candidatus Accumulibacter phosphatisclade IIA str.and the Fe (III) reduction of Rhodoferax ferrireducens influence the chemical composition of the groundwater.

Key words: DGGE, artificial recharge, groundwater, microbial community

中图分类号:

P641.8

苏小四, 孟祥菲, 张文静, 石旭飞, 何海洋. 人工回灌过程中地下水微生物群落变化[J]. 吉林大学学报(地球科学版), 2015, 45(2): 573-583.

Su Xiaosi, Meng Xiangfei, Zhang Wenjing, Shi Xufei, He Haiyang. Change of the Groundwater Microbial Community During Artificial Recharge Process[J]. Journal of Jilin University(Earth Science Edition), 2015, 45(2): 573-583.

参考文献

[1] 杨新安, 于敏, 程军.中国沿海城市地质环境灾害及其防治对策[J].辽宁工程技术大学学报:自然科学版, 1999, 18(5): 497-499. Yang Xin'an, Yu Min, Cheng Jun.Geological Hazards and Countermeasures in Coastai Cities[J].Journal of Liaoning Technical University: Natural Science, 1999, 18(5):497-499.

[2] 龚士良, 李培超, 吴继红, 等.上海地面沉降地下水渗流场与地层应力场分析[J].辽宁工程技术大学学报:自然科学版, 2009, 28(增刊): 232-235. Gong Shiliang, Li Peichao, Wu Jihong, et al.Analysis of Groundwater Seepage Field and Stratum Stress Field of Land Subsidence in Shanghai[J].Journal of Liaoning Technical University:Natural Science, 2009, 28(Sup.):232-235.

[3] 魏子新, 王寒梅, 吴建中, 等.上海地面沉降及其对城市安全影响[J].上海地质, 2009, 30(1):34-39. Wei Zixin, Wang Hanmei, Wu Jianzhong, et al.Land Subsidence and Its Influences on Urban Security of Shanghai[J].Shanghai Geology, 2009, 30(1):34-39.

[4] 苏小四, 谷小溪, 孟婧莹, 等.人工回灌条件下多组分溶质的反应迁移模拟[J].吉林大学学报:地球科学版, 2012, 42(2):485-491. Su Xiaosi, Gu Xiaoxi, Meng Jingying, et al. Fate and Transport Simulation of Multi-Component Solute Under Artificial Recharge Conditions[J]. Journal of Jilin University: Earth Science Edition, 2012, 42(2):485-491.

[5] 杜新强, 齐素文, 廖资生, 等.人工补给对含水层水质的影响[J].吉林大学学报:地球科学版, 2007, 37(2):293-297. Du Xinqiang, Qi Suwen, Liao Zisheng, et al. Influence of Artificial Recharge on Groundwater Quality[J]. Journal of Jilin University: Earth Science Edition, 2007, 37(2):293-297.

[6] 张学真.地下水人工补给研究现状与前瞻[J].地下水, 2005, 27(1):25-28. Zhang Xuezhen. Research Situation and Prospective of Artificial Groundwater Recharge[J].Groundwater, 2005, 27(1):25-28.

[7] 谢娟, 姜凌, 李泉.地下水人工补给水质的研究:以西安市回灌为例[J].西安工程学院学报, 2002, 24(4):67-72. Xie Juan, Jiang Ling, Li Quan. The Quality of Artificially Recharged Groundwater: Taking Artificially Recharged Groundwater in Xi'an as an Example[J]. Journal of Xi'an Engineering University, 2002, 24(4):67-72.

[8] 路莹, 杜新强, 范伟, 等.地下水人工回灌过程中微生物堵塞的预测[J].湖南大学学报:自然科学版, 2012, 39(1): 77-80. Lu Ying, Du Xinqiang, Fan Wei, et al.Prediction of Microbial Clogging in Groundwater Artificial Recharge[J]. Journal of Hunan University: Natural Sciences, 2012, 39(1): 77-80.

[9] 姜桂华, 廖资生, 徐凌云, 等.人工微生物脱氮过程中含水层堵塞问题的实验研究[J].长春科技大学学报, 1998, 28(2): 67-71. Jiang Guihua, Liao Zisheng, Xu Lingyun, et al.The Experimental Study on Clogging of Aquifer During the Treatment of Nitric Pollution by Synthetic Microorganism[J].Journal of Changchun University of Science and Technology, 1998, 28(2): 67-71.

[10] Muyzer G, De Waal E C, Uitterlinden A G. Profiling of Complex Microbial Populations by Denaturing Gradient Gel Electrophoresis Analysis of Polymerase Chain Reaction-Amplified Genes Coding for 16S rRNA[J]. Applied and Environmental Microbiology, 1993, 59(3): 695-700.

[11] Muyzer G, Smalla K. Application of Denaturing Gradient Gel Electrophoresis (DGGE) and Temperature Gradient Gel Electrophoresis (TGGE) in Microbial Ecology[J]. Antonie van Leeuwenhoek, 1998, 73(1): 127-141.

[12] 马悦欣, Holmstrm C, Webb J, 等.变性梯度凝胶电泳(DGGE)在微生物生态学中的应用[J].生态学报, 2003, 23(8):1561-1569. Ma Yuexin, Holmstrm C, Webb J, et al.Application of Denaturing Gradient Gel Eletrophoresis(DGGE) in Microbial Ecology[J].Acta Ecologica Sinica, 2003, 23(8):1561-1569.

[13] 卢永, 陈秉娟, 申世峰, 等.PCR-DGGE在水处理微生物群落多样性分析中的应用[J].化学与生物工程, 2009, 26(5): 55-59. Lu Yong, Chen Bingjuan, Shen Shifeng, et al.The Application of PCR-DGGE in the Abalysis of Microbial Community Diversity in Water Treatment System[J].Chemistry & Bioengineering, 2009, 26(5):55-59.

[14] 李怀, 关卫省, 欧阳二明, 等.DGGE技术及其在环境微生物中的应用[J].环境科学与管理, 2008, 33(10):93-96, 99. Li Huai, Guan Weisheng, Ouyang Erming, et al.DGGE(Denaturing Gradient Gel Eletrophoresis) and Its Application in the Research of Environnmental Microbiology[J].Environmental Science and Management, 2008, 33(10):93-96, 99.

[15] 刘正辉.东江氨氮污染河段的微生物群落特征[D].广州:华南理工大学, 2011. Liu Zhenghui. Microbial Community Characteristics in the Water Column Contaminated with Ammonia-Nitrogen Along the Dongjiang River[D].Guangzhou:South China University of Technology, 2011.

[16] 王威.浅层地下水中石油类特征污染物迁移转化机理研究[D].长春:吉林大学, 2012. Wang Wei.Research on the Migration and Transformation Mechanism of Petroleum Characteristic Contaminants in Sallow Groundwater[D].Changchun:Jilin University, 2012.

[17] 安永磊.原位生物修复硝基苯污染地下水微生物群落结构及修复效能[D].长春:吉林大学, 2012. An Yonglei.The Microbial Community Structure and Remediation Efficiency in In-Situ Bioremediation of Nitrobenzene Contaminated Groundwater[D]. Changchun:Jilin University, 2012.

[18] 林学钰, 张文静, 何海洋, 等.人工回灌对地下水水质影响的室内模拟实验[J].吉林大学学报:地球科学版, 2012, 42(5): 1404-1409, 1433. Lin Xueyu, Zhang Wenjing, He Haiyang, et al. Experiment on Impact of Groundwater Quality During Artificial Recharge Process[J]. Journal of Jilin University: Earth Science Edition, 2012, 42(5): 1404-1409, 1433.

[19] 石旭飞, 张文静, 王寒梅, 等.人工回灌过程中的水-岩相互作用模拟[J].吉林大学学报:地球科学版, 2013, 43(1): 220-227, 234. Shi Xufei, Zhang Wenjing, Wang Hanmei, et al. Modeling of Water-Rock Interaction During the Artificial Recharge[J]. Journal of Jilin University: Earth Science Edition, 2013, 43(1): 220-227, 234.

[20] 石旭飞, 王寒梅, 焦珣, 等.人工回灌条件下的水岩作用室内实验研究[J].上海国土资源, 2012, 33(2):21-24, 73. Shi Xufei, Wang Hanmei, Jiao Xun, et al.Experimental Study of Water-Rock Interaction During Artificial Recharge[J].Shanghai Land & Resources, 2012, 33(2):21-24, 73.

[21] 罗海峰, 齐鸿雁, 薛凯, 等.PCR-DGGE技术在农田土壤微生物多样性研究中的应用[J].生态学报, 2003, 23(8):1570-1575. Luo Haifeng, Qi Hongyan, Xue Kai, et al.A Preliminary Applicationg of PCR-DGGE to Study Microbial Diversity in Soil[J].Acta Ecologica Sinica, 2003, 23(8):1570-1575.

[22] 吕新, 陈丽华, 李玥仁.4种不同土壤微生物DNA提取方法对DGGE分析微生物群落的影响[J].福建农业学报, 2012, 27(4): 367-372. Lü Xin, Chen Lihua, Li Yueren.Effect of DNA Extraction on DGGE Analysis of Microbial Community in Soil[J].Fujian Journal of Agricultural Sciences, 2012, 27(4): 367-372.

[23] 殷峻, 陈英旭, 刘和, 等.应用PCR-DGGE技术研究处理含氨废气的生物滤塔中微生物多样性[J].环境科学, 2004, 25(6): 11-15. Yin Jun, Chen Yingxu, Liu He, et al.Preliminary Application of PCR-DGGE to Analyzing Microbial Diversity in Biofilters Treating Air Loaded with Ammonia[J].Environmental Science, 2004, 25(6): 11-15.

[24] 刘新春, 吴成强, 杨清香, 等.PCR-DGGE方法的建立及其在微生物生态群落分析中的应用探讨[C]//张洪勋, 庄绪亮. 微生物生态学研究进展:第五届微生物生态学术研讨会论文集.北京:中国生态学学会, 2003:32-40. Liu Xinchun, Wu Chengqiang, Yang Qingxiang, et al.The Discussion of the Establishment of the PCR-DGGE Method and Its Application in the Analysis of Microbial Community[C]//Zhang Hongxun, Zhuang Xuliang.The Progress of Microbial Ecology Research: The Collected Papers of the Fifth Microbial Ecology Seminar. Beijing: Ecological Society of China, 2003: 32-40.

[25] 蒙嵘, 浦跃武, 任敦建, 等.PCR-DGGE技术分析染整废水微生物群落多样性[J].生物技术通报, 2012(10): 137-141. Meng Rong, Pu Yuewu, Ren Dunjian, et al.Application of PCR-DGGE to Analysis of Microbial Community Diversity in Dyeing Wastewater[J].Biotechnology Bulletin, 2012(10): 137-141.

[26] 郑昭贤.石油污染浅层地下水中氯代烷烃降解的微生物响应规律研究[D].长春:吉林大学, 2012. Zheng Zhaoxian.Microbial Response Law of Natural Biodegradation of Chloralkane Solvents in a Petroleum Hydrocarbon Contaminated Shallow Groundwater[D].Changchun:Jilin University, 2012.

[27] Masai E, Katayama K, Fukuda M. Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds[J]. Bioscience, Biotechnology, and Biochemistry, 2007, 71(1): 1-15.

[28] Bifulco J, Shirey J, Bissonnette G.Detection of Acine-tobacter Spp in Rural Drinking Water Supplies[J]. Applied and Environmental Microbiology, 1989, 55(9): 2214-2219.

[29] Nagashima S, Kamimura A, Shimizu T, et al.Complete Genome Sequence of Phototrophic Betaproteobacterium Rubrivivax Gelatinosus IL144[J]. Journal of Bacteriology, 2012, 194(13): 3541-3542.

[30] Risso C, Sun Jun, Zhuang Kai, et al. Genome-Scale Comparison and Constraint-Based Metabolic Reconstruction of the Facultative Anaerobic Fe(III)-Reducer Rhodoferax Ferrireducens[J]. BMC Genomics, 2009, 10(1): 447-465.

[31] 石旭飞. 人工回灌过程中地下水重金属迁移转化机理:以上海某回灌场地为例[D].长春:吉林大学, 2013. Shi Xufei. Migration and Transformation Mechanism of Heavy Metals During the Process of Artificial Recharge:A Case Study in an Artificial Recharge Test Site in Shanghai[D].Changchun:Jilin University, 2013.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed