Journal of Jilin University(Earth Science Edition) ›› 2017, Vol. 47 ›› Issue (2): 573-579.doi: 10.13278/j.cnki.jjuese.201702206

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Biodegradation Property of Phenol Using the Immobilized Tyrosinase on Carbon Material

Liu Na1, Liang Gang1, Dong Xinwei1, Qi Xiaoli1, Yang Yuesuo1,2, Ye Kang1, Piao Yunxian1   

  1. 1. Key Laboratory of Groundwater Resources and Environment of the Ministry of Education/College of Environment and Resources, Jilin University, Changchun 130021, China;
    2. Key Lab of Eco-Restoration of Regional Contaminated Environment, Shenyang University, Ministry of Education, Shenyang 110044, China
  • Received:2016-06-30 Online:2017-03-26 Published:2017-03-26
  • Supported by:
    Supported by the National Natural Science Foundation of China (41472237) and the Natural Science Foundation from the Department of Science & Technology of Jilin Province (20150101094JC)

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Abstract: Graphene oxide, a novel carbon material, was employed as the supporting material for the immobilization of tyrosinase enzymes with covalent bonding method. Loading dosage, enzyme activity, optimal conditions for phenol degradation and storage stability of the immobilized tyrosinase were investigated. Loading yield and enzyme activity of immobilized tyrosinase could reach 1.78 mg/mg support and 1 880.6 U/mg support, respectively. The phenol degradation efficiency by immobilized tyrosinase was 86.3% in 30 h reaction and the optimal conditions of phenol degradation were at pH 7.0 and 25℃. Comparing with the free tyrosinase, the immobilized tyrosinase had better storage stability by retaining 77.7% of initial activity for at least 30 days when stored at 4℃. With the introduced magnetic particles in the surface of graphene oxide, the process of enzyme immobilization could be simplified and it is helpful for reuse.

Key words: graphene oxide, tyrosinase, phenol, biodegradation, immobilization

CLC Number: 

  • X506
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