Journal of Jilin University(Earth Science Edition) ›› 2015, Vol. 45 ›› Issue (4): 1198-1204.doi: 10.13278/j.cnki.jjuese.201504206

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Centrifugation Effect on the Functional Diversity of Microbial Community in Fallen Leaves Displaying in BIOLOG Micro-Plates

Li Yumei1,2, Luo Mingqi1,2, Pan Guoyong1,2, Tao Qianye1,2   

  1. 1. Key Laboratory of Computational Geodynamics, Chinese Academy of Sciences, Beijing 100049, China;
    2. College of Earth Science, University of Chinese Academy Sciences, Beijing 100049, China
  • Received:2014-10-28 Published:2015-07-26

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Abstract:

The decomposition of organic matter in terrestrial ecosystem is an important link in the global carbon cycle; however, and the knowledge of this process are still not sufficient. One of the reasons is the lack of a systematical recognition to the changes of microbial community functional diversity during a decomposition of plant litter. Things will get better if the method of BIOLOG micro-plates can be introduced into the initial degradation process of plant litter. Unfortunately, there was no conclusion on how to use this method to the initial degradation, and whether a centrifugal operation should be used during the process of pretreatment. We choose the eluting solution of Beijing birch forest litter as inoculums and consider the effects of centrifugal operation on the BIOLOG micro-plates. The results show that centrifugation can reduce the turbidity (absorbance decreases 0.13) and the interference of color to the solution. At the same time, centrifugation could reduce the measured quantity of microbial communities (AWCD value can be reduced by 0.4). The less the quantity of microbial communities in the sample is, the greater the impact on the functional diversity of soil microbial communities is. Therefore, a specific study target should be the judging basis to determine whether a centrifugation is used for its pretreatment.

Key words: BIOLOG, centrifugation, leaf litter, microbial community, functional diversity

CLC Number: 

  • S718.43

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