Based on lithology and 14C dating survey, a spore-pollen analysis of Holocene sediments in northern Henan Plain was conducted. 6 grass, species, 3 species broad-leaved tree species, 2 gymnosperm tree species, and 2 fern species are found. The spore-pollen assemblages are divided into 6 zones that demonstrate a history of palaeoclimate and palaeoenvironment changes, which indicates an evolvement trend of cool-wet→warm-humid→cool-dry. 10 200-8 200 cal BP,the temperature and humidity were both low, a significant cooling event could be found in 8 200 cal BP;8 200-4 000 cal BP,the spore-pollen assemblage indicated the humid-warm climate in the mid Holocene;4 000-3 300 cal BP,the humidity was lower than that of 4 000 cal BP, the paleoclimate was becoming cold-dry;3 300-2 400 cal BP,the palaeoclimate remained cold-dry in this period; 2 400-1 350 cal BP, climate got warm and humid; from 1 350 cal BP up to now, temperature fell down. The corresponding Peiligang culture, Yangshao culture and Longshan culture, showed a "less developed→prosperous→decayed" trend. The Holocene climate changes had a decisive influence on the ancient culture succession. During the period of high temperature and humidity, the increasing of vegetation, primitive farming productivity, population, and ancient cultural sites indicates the ancient cultural prosperity. On the contrary, primitive farming productivity, population and ancient cultural sites decreased when temperature and humidity drop. This indicates a decay of the ancient cultures. Generally speaking, ancient cultural development corresponds to the paleoclimate changes.