›› 2012, Vol. 42 ›› Issue (04): 1071-1076.

Previous Articles    

Technology optimization of Sialic acid isolation from hen eggshell membrane by quadric regression orthogonal rotary tests

LIU Jing-bo, TAO Xu, JIANG Wei, DING Long, WANG Er-lei   

  1. Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, China
  • Received:2011-08-25 Online:2012-07-01 Published:2012-07-01

PDF (PC)

520

Abstract: On the basis of single factor test, the orthogonal rotary test was employed to optimize the process of sialic acid isolation from hen eggshell membrane. Four factors: temperature, time, pH value and liquid-to-material ratio, were investigated in order to achieve best experiment results. The order of the importance of the factors was temperature, time, pH value and liquid-to-material ratio. The optimal values of the factors were: temperature of 83℃, time of 2.1 h, pH value 1.4 and liquid-to-material ratio of 101 mL/g. Under this condition the yield of sialic acid was 85%. This procedure is steady and efficient. The experiment results were consistent with the model prediction.

Key words: food processing technology, hen eggshell membrane, sialic acid, extraction

CLC Number: 

  • TS253.9
[1] 陶旭,刘静波,王二雷,等.废弃鸡蛋壳的化学组成及其中唾液酸的生物学功能[J]. 食品科学,2010,11:382-385. Tao Xu, Liu Jing-bo, Wang Er-lei, et al. Chemical composition of wasted eggshell and biological function of sialic acid in eggshell[J]. Food Science, 2010,11:382-385.
[2] Asakuma S, Ueda Y, Akiyama F, et al. Short communication:Effect of grazing on the concentrations of total sialic acid and hexose in bovine milk[J].Journal of Dairy Science,2010,93(10):4850-4854.
[3] Nakano T, Kusump S, Ikawa S, et al.Detection of whey in margarine by analysis of sialic acid and kappa-casein glycomacropeptide[J]. Milchwissenschaft-milk Science International,2006, 61(1):36-40.
[4] 仇英海,詹晓北,于军华,等. 由鸡蛋黄粉制备含唾液酸的水解液[J].食品科技,2003(2):28-30. Chou Ying-hai, Zhan Xiao-bei, Yu Jun-hua, et al. Production of sialic acid-containing hydrolysatefrom hen egg yolk powder[J]. Food Science and Technology,2003(2).28-30.
[5] 李文强. 唾液酸发酵控制及其分离纯化的研究. 无锡:江南大学生物工程学院, 2005. Li Wen-qiang. Fermentation process control, isolation and purfication of sialic acid.Wuxi:School of Biotechnology,Jiangnan University,2005.
[6] Nakano T, Ikawa N I, Ozimek L. Chemical composition of chicken eggshell powder[J]. Poultry Science, 2003, 82(3):510-514.
[7] Lacomba Ramon, Salcedo Jaime, Alegria Amparo. Determination of sialic acid and gangliosides in biological samples and dairy products:A review[J]. Journal of Pharmaceutical and Biomedical Analysis, 2010,51(2):346-357.
[8] 任露泉.试验优化设计与分析[M].北京:高等教育出版社,2003.
[9] 冯宗帅,吴彩娥,方升佐,等.青钱柳叶总黄酮超声辅助提取工艺优化[J].农业机械学报,2009,40(2):130-134. Feng Zong-shuai, Wu Cai-e,Fang Sheng-zuo, et al. Technology optimization of total flavonoids extraction from cyclocarya paliurus leaves by ultrasonic assistance[J]. Transactions of the Chinese Society for Agricultural Machinery,2009, 40(2):130-134.
[1] OUYANG Dan-tong, FAN Qi. Clause-level context-aware open information extraction [J]. Journal of Jilin University(Engineering and Technology Edition), 2018, 48(5): 1563-1570.
[2] GENG Qing-tian, YU Fan-hua, WANG Yu-ting, GAO Qi-kun. New algorithm for vehicle type detection based on feature fusion [J]. 吉林大学学报(工学版), 2018, 48(3): 929-935.
[3] DONG Qiang, LIU Jing-hong, ZHOU Qian-fei. Improved SURF algorithm used in image mosaic [J]. 吉林大学学报(工学版), 2017, 47(5): 1644-1652.
[4] XU Meng-lei, GAO Yu, LIU Jing-bo, XIONG Jin-feng, ZHAO Song-ning, HUANG Yan-jun. Neuroprotective effects of soy isoflavones on PC12 cells based on antioxidation [J]. 吉林大学学报(工学版), 2017, 47(1): 332-336.
[5] YIN Ming, ZHAN Yin-wei, PEI Hai-long. Co-sparse analysis operator learning for image fusion [J]. 吉林大学学报(工学版), 2016, 46(6): 2052-2058.
[6] LI Hui, WANG Yan-jiang, LIU Bao-di, LIU Wei-feng, WANG Xiao-meng. Extraction algorithm of salient object by fast dictionary learning and feature rarity [J]. 吉林大学学报(工学版), 2016, 46(5): 1710-1717.
[7] XIAO Zhong-jie. Recognition of digital image based on wavelet space feature spectrum entropy [J]. 吉林大学学报(工学版), 2015, 45(6): 1994-1998.
[8] ZHU Qi-dan, XU Cong-ying, CAI Cheng-tao. Electronic image stabilization algorithm for on board catadioptric omnidirectional vision system [J]. 吉林大学学报(工学版), 2015, 45(4): 1288-1296.
[9] ZHANG Tie-hua, JIANG Nan, LIU Di-ru, HAO Jin-feng, MAO Chun-ling, GUO Ming-ruo. Preparation of whey protein based fat replacer and application in low fat milk [J]. 吉林大学学报(工学版), 2015, 45(3): 1024-1028.
[10] LIU Hong,SUN Shuang-zi,WANG Qing-yuan,LI Yan-zhong. PSO based feature extraction method for analog circuit fault information [J]. 吉林大学学报(工学版), 2015, 45(2): 675-680.
[11] PAN Hai-yang, LIU Shun-an, YAO Yong-ming. Depth information-basd autonomous aerial refueling [J]. 吉林大学学报(工学版), 2014, 44(6): 1750-1756.
[12] YUAN Yuan,ZHANG Huan-jie, MIAO Yu-tian,WU Si-jia, LIN Song-yi,LIU Jing-bo. Contribution of the frying condition of potato slices to the formation of acrylamide [J]. 吉林大学学报(工学版), 2014, 44(5): 1525-1530.
[13] ZHAO Mou-ming,LONG Zhao,ZHAO Qiang-zhong,XU Ju-cai. Effects of protein content and composition on the physicochemical properties of whipping cream [J]. 吉林大学学报(工学版), 2014, 44(5): 1531-1536.
[14] GU Bo-yu,SUN Jun-xi,LI Hong-zuo,LIU Hong-xi,LIU Guang-wen. Face recognition based on eigen weighted modular two-directional two-dimensional PCA [J]. 吉林大学学报(工学版), 2014, 44(3): 828-833.
[15] JIN Sheng-lang, WANG Ying, YIN Yong-guang. Effect of high intensity pulsed electric field pretreatment on elasticity of starch-myofibril protein mixed gelatin [J]. 吉林大学学报(工学版), 2014, 44(2): 573-578.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © Journal of Jilin University(Engineering and Technology Edition), All Rights Reserved.
Tel: +86-431-85095297　Fax: +86-431-85094074　E-mail: xbgxb@jlu.edu.cn
Powered by Beijing Magtech Co. Ltd