吉林大学学报(工学版)

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Extraction of total flavones from Rhodiola sachalinensis
by cellulase and ethanol solution

Bi Hui-min, Zhang Shou-qin, Liu Chang-jiao   

  1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China
  • Received:2006-04-03 Revised:2006-06-18 Online:2007-03-01 Published:2007-03-01
  • Contact: Zhang Shou-qin

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Abstract: Rhodiola sachalinensis was treated in cellulase and ethanol solution, the leaching rate of total flavones was measured under different treatment conditions. The extraction effects of different methods and the DPPH radical scavenging activities of the crude extracts were compared. Experiment results show that the optimum extraction parameters are:36% volume fraction of ethanol, 2.0% dry weight of cellulase, liquid to solid ratio of 52∶1,pH 5.5, enzyme digestion temperature of 50 ℃ and extraction time of 5 h. Under such conditions the leaching rate of total flavones is up to 7.144%.The cellulaseethanol extraction method shows high effectiveness, the crude extract has the highest yield with stronger DPPH radical scavenging activity.

Key words: food science and technology, flavones, enzymatic process, Rhodiola sachalinensis, cellulase, DPPH radical scavenging activity

CLC Number: 

  • TS201.2
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