4种含RGD的短肽对细胞粘附作用的影响

4种含RGD的短肽对细胞粘附作用的影响

黄宜兵¹, 吴胜男², 徐力¹, 张学忠¹

1. 吉林大学分子酶学工程教育部重点实验室，长春 130021； 2. 华南师范大学激光生命科学研究所，激光生命科学教育部重点实验室，广州 510631

收稿日期:2006-07-17 修回日期:1900-01-01 出版日期:2007-01-26 发布日期:2007-01-26
通讯作者: 张学忠

Effects of Motif RGDcontaining Small Peptides on Cell Adhesion

HUANG Yibing¹， WU Shengnan²， XU Li¹， ZHANG Xuezhong¹

1. Key Laboratory of Molecular Enzymology and Engineering， Ministry of Education， Jilin University，  Changchun 130021， China； 2. MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science， South China Normal University， Guangzhou 510631， China

Received:2006-07-17 Revised:1900-01-01 Online:2007-01-26 Published:2007-01-26
Contact: ZHANG Xuezhong

摘要/Abstract

摘要： 从两方面对比研究了4种含Arg-Gly-Asp(RGD)的细胞粘附肽RGD,RGDS,RGD-(NH₂)₂即RGE-NH_{2_{和RGDS-NH_{2_{对细胞粘附的影响. 一方面研究了固定在聚乙交酯丙交酯共聚物（PLGA）膜上的4种细胞粘附肽通过其细胞定向作用对大肠癌细胞（HCT-8）和人成骨肉瘤细胞（OS732）细胞粘附性
的促进作用; 另一方面研究了外源性细胞粘附肽对HCT-8和OS732在纤维连接蛋白（FN）上粘附的竞争性抑制作用. 结果发现，除了RGD-(NH₂)₂，其他3种肽都具有明显抑制细胞粘附的作用且具有一定的浓度依赖关系，其中以RGDS和RGDS-NH₂的能力最强，RGD稍弱. 对于HCT-8细胞，3种肽的最大抑制率分别为53.3%，
56.3%，37.5%；而对OS732细胞，3种肽的最大抑制率分别为50.9%，49.8%，34%.}}}}

关键词: 细胞粘附肽, Arg-Gly-Asp, 聚乙交酯丙交酯共聚物, 大肠癌细胞, 人成骨肉瘤细胞, 纤维连接蛋白

Abstract: The cell adhesive effects of four peptides containing RGD，RGDS，RGD-(NH₂)₂ and RGDS-NH₂ on Human Intestines Cancer (HCT-8)， Human Osteogenic Sarconia (OS732) were investigated. First of all， these cell adhesion peptides were immobilized on PLGA blend membrane respectively and their effects on cell adhesion were investigated. The four peptides except RGD-(NH₂)₂ on the membrane can promote the cell adhesion to the membrane. On the other hand，MTT assay was used to examine the adhesion of HCT-8 cells and OS732 cells on the Fibronectin (FN) after treatment with cellular adhesion peptide， we found that cellular adhesion peptides RGDS，RGDS-NH ₂，RGD significantly inhibited the adhesion of HCT-8 cells and OS732 cells on the FN in dosedependent manner. RGD-(NH₂)₂ does not show an obvious ability to inhibit cell adhesion.The maximum percentages of inhibition were 53.3%，56.3%，37.5% on HCT8 and 50.9%，49.8%，34% on OS732 by RGDS，RGDS-NH₂，RGD，respectively. 

Key words: cellular adhesion peptide, RGD, PLGA, HCT-8, OS732, fibronectin

中图分类号:

黄宜兵, 吴胜男, 徐力, 张学忠. 4种含RGD的短肽对细胞粘附作用的影响[J]. J4, 2007, 45(01): 128-134.

HUANG Yibing， WU Shengnan， XU Li， ZHANG Xuezhong. Effects of Motif RGDcontaining Small Peptides on Cell Adhesion[J]. J4, 2007, 45(01): 128-134.