基于全内反射荧光显微镜的单个心肌细胞内钙信号斑图的介观表征

J4 ›› 2010, Vol. 07 ›› Issue (4): 672-675.

基于全内反射荧光显微镜的单个心肌细胞内钙信号斑图的介观表征

白永强¹, 朱星²

1. 大庆石油学院电子科学学院, 黑龙江大庆 163318;2. 北京大学物理学院人工微结构与介观物理国家重点实验室, 北京 100871

收稿日期:2009-12-18 出版日期:2010-07-26 发布日期:2011-06-14
通讯作者: 白永强 E-mail:yongqiangbai@gmail.com

Mesoscopic Representation on |Patterns of Ca2+ Signals in Single Heart Cell Based on |Total Internal Reflection Fluorescence Microscope

BAI Yong qiang¹, ZHU Xing²

1. College of Electronic Science, Daqing Petroleum Institute, Daqing 163318, Heilongjiang Province, China|2. School of Physics, State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, Peking University, Beijing 100871, China

Received:2009-12-18 Online:2010-07-26 Published:2011-06-14
Contact: BAI Yong qiang E-mail:yongqiangbai@gmail.com

摘要/Abstract

摘要：

利用基于近场光学原理组建的全内反射荧光显微镜研究单个大鼠心肌细胞内钙离子信号的斑图. 结果表明: 存在于盖玻片表面约100 nm区域的隐失场可较好地照射活体细胞, 并能清晰地显示胞内信号; 心肌细胞内钙信号包括钙火花、钙靶波、钙平面波和钙螺旋波等形式, 钙信号各形式间存在相互作用; 在钙诱导钙释放机制作用下, 心肌细胞具有较强的可激发特性.

关键词: 全内反射荧光显微镜, 钙火花, 钙螺旋波, 钙诱导钙释放, 可激发特性

Abstract:

The signals of calcium ions in single rat heart cell were studied by means of a total internal reflection fluorescence microscope made by the theory of near-field optics. The results show that Ca²⁺ signals can be detected clearly under the illumination of evanescence which only penetrated cell about 100 nm. Ca²⁺ signals in single heart cell show Ca²⁺ sparks, Ca²⁺ target waves, Ca²⁺ plane waves and Ca²⁺ spiral waves, and these forms interact to each other. Further more, single heart cells show strong excitability under calcium\|induced calcium release mechanism.

Key words: total internal reflection fluorescence microscope (TIRFM), Ca2+ spark, Ca2+ spiral wave, calcium\, induced calcium release (CICR), excitability

中图分类号:

O433

白永强, 朱星. 基于全内反射荧光显微镜的单个心肌细胞内钙信号斑图的介观表征[J]. J4, 2010, 07(4): 672-675.

BAI Yong-Jiang, SHU Xing. Mesoscopic Representation on |Patterns of Ca2+ Signals in Single Heart Cell Based on |Total Internal Reflection Fluorescence Microscope[J]. J4, 2010, 07(4): 672-675.