谷胱甘肽过氧化物酶模拟物6-ySeCD对过氧化氢诱导小鼠成纤维细胞损伤的保护作用

J4 ›› 2010, Vol. 48 ›› Issue (02): 329-332.

谷胱甘肽过氧化物酶模拟物6-ySeCD对过氧化氢诱导小鼠成纤维细胞损伤的保护作用

徐亚维^1,2, 闫岗林¹, 龚平生¹, 牟颖1, 罗贵民¹, 杨佳³, 吕绍武¹

1. 吉林大学分子酶学工程教育部重点实验室, 长春 130021|2. 吉林农业科技学院生物工程系, 吉林吉林 132001|3. 长春西诺生物科技有限公司| 长春 130021

收稿日期:2009-04-20 出版日期:2010-03-26 发布日期:2010-03-22
通讯作者: 吕绍武 E-mail:lvsw@mail.jlu.edu.cn

GPX Mimic 6-CySeCD Protecting Mouse Fibroblast |Cell NIH3T3from Damage Induced by H₂O₂

XU Yawei^1,2, YAN Ganglin^1, GONG Pingsheng¹, MU Ying¹, LUO Guimin¹, YANG Jia³, LvShaowu¹

1. Key Laboratory for Molecular Enzymology and Enginnering of Ministry of Education, Jilin University, Changchun 130021, China|2. Department of Bioengineering, Jilin Agricultural Science and Technology College, Jilin 132001, Jilin Province, China|3. Fassino Bio\|Technology Co.Ltd, Changchun 130021, China

Received:2009-04-20 Online:2010-03-26 Published:2010-03-22
Contact: Lv shao wu E-mail:lvsw@mail.jlu.edu.cn

摘要/Abstract

摘要：

考察不同剂量的H₂O₂作用于小鼠成纤维细胞（NIH3T3）后, 细胞脂质过氧化、存活率、 DNA片段化的变化情况, 并研究谷胱甘肽过氧化物酶（GPX）的模拟物6A,6B-环己胺-6A′,6B′-硒桥联-β-CD（6-CySeCD）抗H₂O₂诱导小鼠成纤维细胞损伤的能力. 结果表明: H₂O₂对NIH3T3细胞有严重损伤; 6-CySeCD能有效保护细胞, 防止紫外线引起的损伤.

关键词: 谷胱甘肽过氧化物酶(GPX)；模拟物； 6CySeCD；过氧化氢；成纤维细胞

Abstract:

The antioxidant effect of 6A,6A′-cyclohexylamine-6B,6B′-seleniumbridged-β-cyclodextrin (6-CySeCD), a new mimic of glutathione peroxidase (GPX), was confirmed with a model system in which mouse fibroblast cell NIH3T3 were damaged by H₂O₂. The cell damage was characterized in terms of the lipid peroxidation of the cells, cell viability, DNA lysis rate. The injury effects of H₂O₂ and protection effects of 6-CySeCD on the cells were studied via the model system. The results show the 6CySeCD could be able to protect cells from the damage induced by H₂O₂.

Key words: glutathione peroxidase (GPX)； mimic； 6CySeCD； hydrogen peroxide, fibroblast cell

中图分类号:

R392.11

徐亚维, 闫岗林, 龚平生, 牟颖, 罗贵民, 杨佳, 吕绍武. 谷胱甘肽过氧化物酶模拟物6-ySeCD对过氧化氢诱导小鼠成纤维细胞损伤的保护作用[J]. J4, 2010, 48(02): 329-332.

XU E-Wei, YAN Gang-Lin, GONG Beng-Sheng, MAO Ying, LUO Gui-Min, YANG Jia, LV Chao-Wu. GPX Mimic 6-CySeCD Protecting Mouse Fibroblast |Cell NIH3T3from Damage Induced by H₂O₂[J]. J4, 2010, 48(02): 329-332.