玉米源活性肽的分离纯化及清除DPPH自由基的活性

J4 ›› 2010, Vol. 48 ›› Issue (02): 333-339.

玉米源活性肽的分离纯化及清除DPPH自由基的活性

李鸿梅^1,2, 李玉扩², 李红², 倪鹏¹, 宋欣¹, 徐力^1

1. 吉林大学分子酶学工程教育部重点实验室, 长春 130021； 2. 吉林农业大学食品科学与工程学院, 长春 130118

收稿日期:2009-06-23 出版日期:2010-03-26 发布日期:2010-03-22
通讯作者: 徐力 E-mail:xuli@jlu.edu.cn

Separation and Purification of Corn Active Peptides andTheir Scavenging Effect on DPPH Radical

LI Hongmei^1,2, LI Yukuo², LI Hong2, NI Peng¹, SONG Xin¹, XU Li¹

1. Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun130021, China； 2. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

Received:2009-06-23 Online:2010-03-26 Published:2010-03-22
Contact: XU Li E-mail:xuli@jlu.edu.cn

摘要/Abstract

摘要：

以脱脂醇溶蛋白为底物进行酶水解反应, 获得了玉米源活性肽组分, 经葡聚糖凝胶和离子交换等方法对产物进一步分离纯化. 以α-生
育酚为阳性对照, 研究了活性肽及其分离纯化组分对2,2-二苯基-1-苦基苯肼（DPPH）自由基的清除活性及其浓度的关系. 结果表明: 活性肽及其分离纯化组分均具有清除DPPH自由基的活性; 经CM纤维素弱阳离子交换层析分离所得组分2对DPPH自由基的清除活性最强, 且清除效果与其浓度及作用时间呈正相关, 分子量主要集中在400~700.

关键词: 玉米源活性肽；分离纯化； 2,2-二苯基-1-苦基苯肼(DPPH)自由基；清除作用

Abstract:

Defatted zein was hydrolyzed by proteolysis with protease. The hydrolysate was refined and freezedried into powder. The resulting hydrolysate was further purified by means of gel filtration, ion exchange chromatography and HPLC. With α-tocopherol as the positive control, the antioxidant abilities of corn peptide and its separated fractions were examined for their effects of scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, dosedependent manner was included. The data reveal that corn peptide and its separated fractions exhibited a potent effect of scavenging DPPH. The most active fraction from the active peptide was fraction 2 separated by CM cation exchange chromatography. Its scavenging effect is positively related to its concentration and the reaction time. The molecularmass distributions of the fraction 2 are mostly in a range from 400~700.

Key words: corn active peptide, separation and purification, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, scavenging effect

中图分类号:

Q516

李鸿梅, 李玉扩, 李红, 倪鹏, 宋欣, 徐力. 玉米源活性肽的分离纯化及清除DPPH自由基的活性[J]. J4, 2010, 48(02): 333-339.

LI Hong-Mei, LI Yu-Guang, LI Gong, NI Feng, SONG Xin, XU Li. Separation and Purification of Corn Active Peptides andTheir Scavenging Effect on DPPH Radical[J]. J4, 2010, 48(02): 333-339.