超声波辅助脂肪酶催化拆分丁酸环氧丙酯

J4 ›› 2011, Vol. 49 ›› Issue (04): 782-786.

超声波辅助脂肪酶催化拆分丁酸环氧丙酯

王佳欣¹, 孙若溪², 荀二娜¹, 张爱军¹, 王任¹, 魏晓飞¹, 王磊¹, 王智¹

1. 吉林大学分子酶学工程教育部重点实验室, 长春 130012;
2. 吉林大学生命科学学院, 长春 130012

收稿日期:2010-10-15 出版日期:2011-07-26 发布日期:2011-08-16
通讯作者: 王磊 E-mail:wanglei@jlu.edu.cn

UltrasoundPromoted Resolution of(R,S)Glycidyl Butyrate Catalyzed by Lipase

WANG Jiaxin^1, SUN Ruoxi², XUN Erna¹, ZHANG Aijun¹, WANG Ren¹,WEI Xiaofei¹, WANG Lei¹, WANG Zhi¹

1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University,Changchun 130012, China|
2. College of Life Science, Jilin University, Changchun 130012, China

Received:2010-10-15 Online:2011-07-26 Published:2011-08-16
Contact: WANG Lei E-mail:wanglei@jlu.edu.cn

摘要/Abstract

摘要：

利用超声波辅助脂肪酶催化拆分丁酸环氧丙酯, 考察了超声发生装置、超声处理方法、超声功率及超声温度等反应条件对酶促拆分反应的影响, 筛选得到最适反应条件. 结果表明, 在最适反应条件下, 超声作用可以使酶活提高近4.3倍, 立体选择性提高5倍.

关键词: 超声；脂肪酶；丁酸环氧丙酯；拆分

Abstract:

The ultrasound was used to promote the resolution of glycidyl butyrate catalyzed by lipase. The effects of ultrasound source, ultrasonic processing method, power and temperature were also investigated to obtain the optimum reaction conditions. Under the optimum conditions, the enzyme activity of BSL2 in the resolution of glycidyl butyrate was enhanced by about 4.3fold and the enantioselectivity increased by about 5.0fold.

Key words: ultrasound； lipase； glycidyl butyrate； resolution

中图分类号:

Q814.9

王佳欣, 孙若溪, 荀二娜, 张爱军, 王任, 魏晓飞, 王磊, 王智. 超声波辅助脂肪酶催化拆分丁酸环氧丙酯[J]. J4, 2011, 49(04): 782-786.

WANG Jia-Xin, SUN Re-Xi, XUN Er-Na, ZHANG Ai-Jun, WANG Lin, WEI Xiao-Fei, WANG Lei, WANG Zhi. UltrasoundPromoted Resolution of(R,S)Glycidyl Butyrate Catalyzed by Lipase[J]. J4, 2011, 49(04): 782-786.