壳聚糖羟丁基衍生物的制备及性质

J4 ›› 2011, Vol. 49 ›› Issue (05): 957-963.

壳聚糖羟丁基衍生物的制备及性质

李晶晶, 党奇峰, 程晓杰, 陈西广

中国海洋大学海洋生命学院, 山东青岛 266003

收稿日期:2011-01-17 出版日期:2011-09-26 发布日期:2011-09-27
通讯作者: 陈西广 E-mail:xgchen@ouc.edu.cn

Preparation and Properties of Hydroxybutyl Chitosan

LI Jingjing, DANG Qifeng, CHENG Xiaojie, CHEN Xiguang

College of Marine Life Science, Ocean University of China, Qingdao 266003, Shandong Province, China

Received:2011-01-17 Online:2011-09-26 Published:2011-09-27
Contact: CHEN Xiguang E-mail:xgchen@ouc.edu.cn

摘要/Abstract

摘要：

以1,2环氧丁烷在碱性条件下的开环产物为醚化剂, 对壳聚糖(CS)分子进行改性, 制备了水溶性羟丁基壳聚糖(HBCS), 并采用红外光谱、固体¹³C核磁共振对产物的分子结构进行表征. 结果表明: 羟丁基成功连接到壳聚糖分子链上; 元素分析法测定产物取代度为1.2; 羟丁基壳聚糖可溶于水并可在较广的pH值范围内溶解; 由于改性反应破坏了壳聚糖的结晶区结构, 因此羟丁基壳聚糖的热稳定性比原料壳聚糖差. 对壳聚糖和羟丁基壳聚糖的抑菌性研究结果表明: 羟丁基壳聚糖能够抑制大肠杆菌和金黄色葡萄球菌的生长, 并随着质量分数的增加抑菌效果明显增强; 相同质量分数条件下羟丁基壳聚糖的抑菌活性比壳聚糖弱.

关键词: 壳聚糖；羟丁基壳聚糖；结构表征；理化性质

Abstract:

Watersoluble hydroxybutyl chitosan(HBCS) was prepared by the reaction of chitosan(CS) with 1,2butane oxidation in an alkali solution. The structure of HBCS was characterized by FTIR and solid state ¹³C NMR, and the results prove that the hydroxybutyl has been successfully connected to chitosan molecular chain. The degree of hydroxybutyl substitution estimated by means of elemental ananlysis is 1.2. Besides, HBCS exhibited good watersolubility and could dissolve in aqueous solutions in a broader pH range. Incomparison with CS, HBCS shows a poorer thermostability. This is because the modified reaction destroys the crystalline region of chitosan. Antibacterial activities of CS and the modified derivative were explored by turbidity method. The results show that HBCS exhibits antibacterial activity against E.coli and S.aureus, and with the increase of concentration, the antibacterial effect enhances obviously. It was found that the antimicrobial activity of HBCS is weaker
than that of CS under the same mass concentrations.

Key words: chitosan； hydroxybutyl chitosan； structure characterization； physiochemical property

中图分类号:

李晶晶, 党奇峰, 程晓杰, 陈西广. 壳聚糖羟丁基衍生物的制备及性质[J]. J4, 2011, 49(05): 957-963.

LI Jing-Jing, DANG Ai-Feng, CHENG Xiao-Jie, CHEN Xi-An. Preparation and Properties of Hydroxybutyl Chitosan[J]. J4, 2011, 49(05): 957-963.