关键词: 木犀草苷, 铜掺杂二氧化硅, 雪菊, 富集, 近红外光谱

Abstract: The prepared copperdoped silica adsorbent was used to enrich luteoloside in Coreopsis tinctoria, and the adsorbents enriched with luteoloside were determined by nearinfrared spectroscopy. After the pretreatment of nearinfrared spectra, a quantitative correction model of luteoloside was established by partial least square regression method for determination and validation. The results show that the adsorption efficiency is 89.7% when the mass of copperdoped silica adsorbent is 0.25 g and the adsorption is oscillated at room temperature for 20 min. The adsorbents enriched with luteoloside can be directly detected by nearinfrared spectra without desorption. After the NIR spectral data are processed with multiple scattering correction and first derivative,  the correlation coefficient between the reference mass concentration and the predicted mass concentration of luteoloside is 0.975 0, the root mean square error of cross validation is 0.959 8 mg/L, within the lower mass concentration range of 1.5—19.5 mg/L, and the recovery rate of luteoloside in prediction set can achieve 82.6%—111.4% in the calibration model.

Key words: luteoloside, copperdoped silica, Coreopsis tinctoria, enrichment, nearinfrared spectroscopy