Abstract:

Four kinds of main hosenkosides named as hosenkosides K,A,C,B were used as the indexes of total hosenkosides extracted from the seeds of Impatiens balsamina L. The effects of the density, pH and the rate of the flow of the solution on the adsorption properties of the resin were investigated through the orthogonal test, establishing the adsorption conditions. The results show that the solutions were combined after filtration and evaporated to the 6 times the material weight in vacuum. The solution at pH 6.0 was passed through the AB8 macroporous resin at a flow rate of 3 volumes of the resin in 1 h, then eluted by 80% ethanol. This way could collect the total hosenkosides, in which the content of the sum of four hosenkosides K,A,C,B was in the highest point.

Key words: Impatiens balsamina L.； total hosenkoside, macroporous resin, orthogonal test