吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

系统性红斑狼疮患者外周血辅助性T细胞亚型和细胞因子的检测及其临床意义

赵令,马宁,刘涛,姜振宇   

  1. 吉林大学第一医院风湿科,吉林 长春 130021
  • 收稿日期:2013-08-19 出版日期:2014-03-28 发布日期:2014-05-28
  • 通讯作者: 姜振宇(Tel:0431-88782060,E-mail:512517403@qq.com) E-mail:512517403@qq.com
  • 作者简介:赵 令(1984-)女,吉林省长春市人,医师,医学博士,主要从事系统性红斑狼疮的研究。
  • 基金资助:

    吉林省科技厅重点科技攻关项目资助课题(20130206023SF)

Detection  of subtypes of T helper cells and their cytokines in peripheral blood of patients with systematic lupus erythematosus and their clinical significances

ZHAO Ling,MA Ning,LIU Tao,JIANG Zhen-yu   

  1. Department of Rheumatology,First Hospital,Jilin University,Changchun 130021,China
  • Received:2013-08-19 Online:2014-03-28 Published:2014-05-28

摘要:

目的:探讨系统性红斑狼疮(SLE)患者外周血中产生白细胞介素22(IL-22)的辅助性T 细胞(Th)亚型Th22、Th17和Th1及其细胞因子的表达水平变化及其临床意义,为治疗SLE提供依据。方法:选取30例初发SLE患者(SLE组)和30名健康对照者(健康对照组),采用流式细胞术检测2组研究对象外周血中Th22、Th17 (IL-22+Th17和IL-22-Th17)、Th1(IL-22+Th1和IL-22-Th1)细胞表达比率;ELISA法检测2组研究对象血浆中IL-22、IL-17和干扰素γ(IFN-γ)等细胞因子的表达水平;细胞比率和细胞因子表达水平之间相关性分析采用Spearman相关检验。结果:与健康对照组比较,SLE组患者外周血中IL-22+Th1、Th17和Th22细胞的比率明显升高(P<0.05),SLE患者血浆中IL-17和IL-22的表达水平亦明显升高(P<0.05);SLE患者Th22细胞比率与Th17(IL-22+和IL-22-)细胞比率之间呈正相关关系(r=0.721,P<0.0001;r=0.222,P=0.0132),Th22细胞比率与血浆中IL-22的水平呈正相关关系(r=0.0326,P<0.0001)。SLE患者IL-22+CD4+T和IL-22-Th17细胞的比率随着疾病活动指数(SLEDAI)的升高而升高(r=0.549,P=0.0003;r=0.231,P=0.028)。有颜面部红斑或脱发、抗核抗体(ANA)阳性的SLE患者外周血中Th22细胞的比率均显著升高(P<0.05);光过敏或抗dsDNA阳性的SLE患者Th17(包括IL-22+和IL-22-Th17)细胞的比率上升(P<0.05)。结论:以Th22为主的IL-22+ CD4+T细胞及其细胞因子IL-22可能在SLE的发病中起致病性作用,并可作为监测疾病严重程度的生物指标。

关键词:  , 系统性红斑狼疮, 辅助性T细胞22, 白细胞介素22

Abstract:

Objective
 To explore the changes and clinical significances of  subtypes of  T helper cells Th22,Th17, and Th1,which can produce interleukin 22(IL-22) and their cytokines in peripheral blood of the patients with systematic lupus erythematosus (SLE),and to provide basis for SLE treatment.Methods 30 patients with new onset SLE(SLE group) and 30 healthy controls(healthy control group) were selected.The expression ratios  of Th22,Th17 (IL-22+ Th17 and IL-22- Th17),Th1(IL-22+Th1 and IL-22- Th1)cells in peripheral blood of the objects were compared by flow cytometry;while the levels of plasma  IL-22,IL-17,and interferon γ(IFN-γ) in the patients and healthy controls were measured by ELlSA method;the correlation between  the ratios of cells and the  expression levels of cytokines was analyzed by  Spearman correlation test.Results Compared with healthy control group,the ratios  of Th22,Th17,and IL-22+Th1 cells in peripheral blood of  the patients in  SLE group were siginificantly higher than those in healthy control group(P<0.05),and the expression levels of plasma  IL-22 and IL-17 of the patients in SLE group were also increased(P<0.05).A statistically significant positive correlation was found between the ratios of Th17 (IL-22+,IL-22-) and Th22 cells of the  patients in SLE group(r=0.721,P<0.0001;r=0.222,P=0.0132).The ratio  of Th22 cells was positively correlated with the level of plasma IL-22 of the patients in SLE group (r=0.0326,P<0.0001).The ratios  of  IL-22+CD4+T and IL-22-Th17 cells in peripheral blood of the patients in SLE group were increased along with the increasing of the disease activity score of SLE (SLEDAI)(r=0.549,P=0.0003;r=0.231 P=0.028).The ratios  of Th22 cells in the peripheral blood of  the SLE patients with facial erythema or hair loss,and  positive  anti nuclear antibody(ANA) were increased (P<0.05).The  ratios of Th17 cells (including IL-22+ and IL-22-Th17) in the SLE patients with light allergies or positive anti dsDNA  were also increased (P<0.05).Conclusion The IL-22+ CD4+T cells mainly including Th22 cells and their  cytokine IL-22 may play an important role in the pathogenesis of SLE,which can be used  as a  bio-indicators for monitoring disease severity.

Key words:  , systematic lupus erythematosus, T helper 22 cell, interleukin-22

中图分类号: 

  • R593.24