吉林大学学报(医学版)

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大鼠孤束核内代谢型谷氨酸受体亚型7和8对心脏伤害性感受的调控作用

刘晓华1,韩曼1,杜剑青2   

  1. 1.陕西中医学院生理学教研室,陕西 咸阳712046;2.西安交通大学医学院生理学与病理生理学系,陕西 西安710061)
  • 收稿日期:2014-01-28 出版日期:2014-09-28 发布日期:2014-09-28
  • 通讯作者: 杜剑青 E-mail:(Tel:029-82657490-802,E-mail:dujianq@mail.xjtu.edu.cn)
  • 作者简介:刘晓华(1980-),女,陕西省旬邑县人,讲师,理学博士,主要从事心血管生理基础方面的研究。 
  • 基金资助:

    国家自然科学基金资助课题(31171068)

 Regulation of metabotropic glutamate subtype 7 and 8 receptors in nucleus tractus solitarius in cardiac nociception in rats


LIU Xiao-hua1,HAN Man1,DU Jian-qing2   

  1. (1.Department of Physiology,Shaanxi University of Chinese Medicine,Xianyang 712046,China;2.Department of Physiology and Pathophysiology,School of Medical Sciences, Xi’an Jiaotong UniversityXi’an 710061,China)
  • Received:2014-01-28 Online:2014-09-28 Published:2014-09-28

摘要:

目的:探讨孤束核(NTS)内第3组代谢型谷氨酸受体(mGluRs)及其亚型7和8对心脏-躯体运动反射(CMR)的影响,阐明NTS内第3组mGluRs及其亚型在心脏伤害性信息调控中的作用。方法:40只SD大鼠随机分为4组,L-(+)-2-amino-4-phosphonobutyric acid (L-AP4)组,NTS内分别微量注射第3组mGluRs 激动剂L-AP4 0.1、1.0、10.0和20.0 nmol;N,N’-diphenylmethyl-1,2-ethanediamine (AMN082) 组,分别注射mGluRs7激动剂AMN082 1、2和4 nmol; (S)-3,4-dicarboxyphenylglycine (DCPG) 组,分别注射mGluRs8激动剂DCPG 4、6和8 nmol;(RS)-α-methylserine-O-phosphate (MSOP)组,分别注射第3组mGluRs 拮抗剂MSOP 20和100 nmol,并于不同时间分别注射MSOP(20 nmol)+L-AP4(10 nmol)、MSOP(20 nmol)+AMN082(2 nmol)和MSOP(20 nmol)+DCPG(6 nmol)。观察各组大鼠CMR的改变。结果:与对照比较,L-AP4组和AMN082组CMR减少(P<0.05);DCPG 组CMR增加(P<0.05);MSOP组注射20 nmol MSOP后CMR无改变(P>0.05),注射100 nmol MSOP后CMR增加(P<0.05);注射20 nmol MSOP后再注射L-AP4或AMN082,CMR无改变(P>0.05)。结论:大鼠NTS内第3组mGluRs对心脏伤害性信息有紧张性抑制作用, mGluR7有抑制作用,而 mGluR8有易化作用。

关键词: 孤束核, 心脏-躯体运动反射, 代谢型谷氨酸受体

Abstract:

Abstract:Objective
To explore the role of metabotropic glutamate receptors (mGluRs) group Ⅲ and its subtypes mGluR7 and mGluR8 in nucleus tractus solitarius(NTS) in cardiac-somatic motor reflex (CMR), and to clarify the modulation role of mGluR Ⅲ and its subtypes in NTS in cardiac nociceptoion.Methods 40 SD rats were randomly divided into L-AP4 group,microinjection of mGluRs Ⅲ agonist L-AP4 0.1,1.0,10.0 or 20.0 nmol in NTS;AMN082 group, microinjection of mGluR7 agonist AMN082 1,2 or 4 nmol;DCPG group,  microinjection of  mGluR8 agonist DCPG 4,6 or 8 nmol;MSOP group,microinjection of   mGluR Ⅲ  antagonist MSOP 20 or 100 nmol,20 nmol MSOP+410 nmol L-AP,20 nmol MSOP+2 nmol AMN082,20 nmol MSOP+6 nmol DCPG.The changes of CMR of the rats in various groups were observed.Results Compared with control,the CMR in L-AP4 and AMN082 groups was decreased (P<0.05);the CMR in DCPG group was increased (P<0.05); the CMR in   MSOP group after injection of 20 nmol MSOP had no change (P>0.05);the CMR in   MSOP group after injection of 100 nmol MSOP was increased (P<0.05);the CMR in MSOP group after injection of 20 nmol MSOP followed L-AP4 or AMNO82 had no change (P>0.05).Conclusion The  group Ⅲ  mGluRs in the NTS play an inhibitory role in cardiac nociception,and mGluR7 has anti-nociceptive effects while mGluR8 has pro-nociceptive effects.

Key words:  , nucleus tractus solitarius, cardiosomatic motor reflex, metabotropic glutamate receptors

中图分类号: 

  • R338