吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

大鼠孤束核内谷氨酸受体亚型对心脏伤害性感受信息的调控作用

刘晓华1,韩曼1,杜剑青2   

  1. 1.陕西中医学院生理学教研室,陕西 咸阳712046;2.西安交通大学医学院生理学与病理生理学系,陕西 西安 710061)
  • 收稿日期:2014-01-28 出版日期:2014-05-28 发布日期:2014-05-28
  • 通讯作者: 杜剑青 E-mail:(Tel:029-82657490-802,E-mail:dujianq@mail.xjtu.edu.cn)
  • 作者简介:刘晓华(1980-),女,陕西省旬邑县人,讲师,理学博士,主要从事心血管生理基础方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(31171068)

Role of glutamate receptor subtypes in cardiac nociception in nucleus tractus solitarius in rats

LIU Xiao-hua1,HAN Man1,DU Jian-qing2   

  1. (1.   Department of Physiology,Shaanxi University of Chinese Medicine,Xianyang 712046,China;2.Department of Physiology and Pathophysiology,School of Medical Sciences,Xi’an Jiaotong University,Xi'an 710061,China)
  • Received:2014-01-28 Online:2014-05-28 Published:2014-05-28

摘要:

目的:探讨大鼠孤束核(NTS)内谷氨酸受体亚型对心包内注射辣椒素诱发的心脏-躯体运动反射(CMR)的影响,阐明NTS对心脏伤害性信息调控的作用机制。方法:SD大鼠60只随机分为鹅膏蕈氨酸(IBO)组、谷氨酸组、5-甲基二氢丙环庚烯亚胺马来酸(MK-801)组、α-甲基,4-羧基苯丙氨酸(MCPG)组、MCPG联合MK-801组和6,7-二硝基喹喔啉-2,3-二酮(DNQX)组;各组大鼠孤束核内分别微量注射13 mmol•L-1 IBO 100 nL,100、200、500 mmol•L-1谷氨酸100 nL,NMDA受体拮抗剂 40和60 mmol•100•-1 MK-801 100 nL,代谢型谷氨酸受体拮抗剂 25 和50 mmol•L-1 MCPG 100 nL,25 mmol•L-1MCPG 50 nL联合40 mmol•L-1MK-801 50 nL,非NMDA受体拮抗剂20和50 mmol•L-1 DNQX 100 nL;观察各组大鼠CMR的变化。结果:与对照组比较, IBO组大鼠CMR减少(P<0.05);谷氨酸组随着谷氨酸浓度的增加,大鼠CMR不断增加(P<0.05); MK-801和MCPG组CMR均减少(P<0.05); MCPG联合MK-801组CMR减少(P<0.05); DNQX组CMR无明显变化(P>0.05)。结论:NTS对心脏伤害性感受信息有易化调控作用,这种易化调节作用主要由NMDA和mGluRs受体介导。

关键词: 孤束核, 心脏-躯体运动反射, 谷氨酸, 谷氨酸受体亚型

Abstract:

Abstract:Objective To study the role of glutamate receptor subtypes in nucleus tractus solitarius(NTS) in cardiac-somatic motor reflex (CMR) induced by intrapericardial administration of capsaicin, and to clarify the modulation mechanism of NTS to cardiac nociceptoion.Methods 60 SD rats were randomly divided into  ibotenic (IBO) group,glutamate group,MK-801 group,MCGP group,MK-801+MCPG group and DNQX group.The NTS microinjected with 130 mmol•L-1  IBO 100 nL, 100,200,500 mmol•L-1 L-glutamate 100 nL,NMDA receptor antagonist 40 and 60 mmol•L-1 MK-801 100 nL,metabotropic glutamate receptors antagonist 25 and 50 mmol•L-1  MCPG 100 nL,25 mmol•L-1   MCPG  50 nL plus 40 mmol•L-1  MK-801 50 nL,non-NMDA receptor antagonist 20 and 50 mmol?L-1DNQX 100 nL,respectively.The changes of CMR of the rats in various groups were observed. Results Compared with control group, the CMR of the rats in IBO group  was decreased (P<0.05);the CMR was increased as the concentration increased in glutamate group(P<0.05);the CMR  in MK-801 and MCPG groups were decreased (P<0.05);the CMR in MK-801+MCPG  group was decreased (P<0.05);the CMR  in DXQX group had no changes (P>0.05).Conclusion  NTS play an facilictory role in cardiac nociception,and the NMDA receptors and mGluRs receptors mediate this facilitory modulation.

Key words: nucleus tractus solitarius, cardiosomatic motor reflex, glutamate, glutamate receptor subtype

中图分类号: 

  • R338.22