吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 287-290.doi: 10.13481/j.1671-587x.20150215

• 基础研究 • 上一篇    下一篇

五味子多糖对人结肠癌HT-29细胞体外增殖及侵袭能力的抑制作用

黄晓东1, 路倩1, 沈楠2, 王艳春1   

  1. 1. 吉林医药学院药理学教研室, 吉林 吉林 132013;
    2. 吉林医药学院机能实验中心, 吉林 吉林 132013
  • 收稿日期:2014-08-26 出版日期:2015-03-28 发布日期:2015-04-04
  • 通讯作者: 王艳春, 教授, 硕士研究生导师(Tel:0432-64560470, E-mail:wangyanchun1972@163.com) E-mail:wangyanchun1972@163.com
  • 作者简介:黄晓东(1978-), 男, 辽宁省抚顺市人, 讲师, 医学博士, 主要从事肿瘤药理学方面的研究。
  • 基金资助:

    吉林省教育厅"十二五"科研项目资助课题(2014547)

Inhibitory effects of Alkaline S.chinenis polysaccharides on proliferation and invasion abilities of colon cancer HT-29 cells in vitro

HUANG Xiaodong1, LU Qian1, SHEN Nan2, WANG Yanchun1   

  1. 1. Department of Pharmacology, Jilin Medical College, Jilin 132013, China;
    2. Function Experiment Center, Jilin Medical College, Jilin 132013, China
  • Received:2014-08-26 Online:2015-03-28 Published:2015-04-04

摘要:

目的:探讨五味子多糖(ASPS)对人结肠癌HT-29细胞增殖和侵袭活性的影响,阐明ASPS的抗肿瘤作用。方法:40只 Wistar大鼠随机分为对照组(给予生理盐水)和100、200、400 mg·kg-1 ASPS组,每组10只,取各组大鼠血清,HT-29细胞体外培养,以各组大鼠血清进行预处理,采用MTT法和Transwell小室检测各组HT-29细胞的增殖抑制率和侵袭活性抑制率;Western blotting法检测各组细胞上清液中bcl-2、bax和Caspase-3蛋白表达水平。结果:与对照组和100 mg·kg-1 ASPS组比较,200和400 mg·kg-1 ASPS组HT-29细胞增殖抑制率和侵袭活性抑制率均升高(P<0.05,P<0.01)。400 mg·kg-1 ASPS组HT-29细胞上清液中bcl-2蛋白表达水平低于其他组(P<0.05,P<0.01),bax和Caspase-3蛋白的表达水平高于对照组(P<0.05,P<0.01)。结论:ASPS能显著抑制细胞的体外增殖和侵袭能力,其机制可能与下调HT-29细胞的bcl-2基因表达,上调bax和Caspase-3基因表达,激活细胞凋亡通道有关联。

关键词: 结直肠肿瘤, 五味子多糖, 侵袭活性, 细胞凋亡

Abstract:

Objective To investigate the effect of Alkaline S.chinenis polysaccharides(ASPS) on the proliferation and invasion abilities of the human colon cancer HT-29 cells,and to clarify the anti-tumor effect of ASPS.Methods 40 Wistar rates were randomly divided into control group(n=10,given saline),and 100,200,400 mg·kg-1 ASPS groups(n=10),respectively.The serum of the rats in various groups were gotten.The HT-29 cells were treated with the serum of the rats in various groups. The inhibitory rates of proliferation and invasion abilities were tested by MTT and Transwell assay;the expression levels of bcl-2,bax,and Caspase-3 proteins in the supernatant of the cells of the rats in various groups were determined by Western blotting method.Results Compared with control group,the inhibitory rates of proliferation and invasion abilities of the HT-29 cells in 200 and 400 mg·kg-1 ASPS groups were increased(P<0.05, P<0.01).The expression level of bcl-2 protein in 400 mg·kg-1 ASPS group was lower than those in other groups (P<0.05),while the expression levels of bax and Caspase-3 proteins were higher than those in control group(P<0.05, P<0.01).Conclusion ASPS can inhibit the proliferation and invasion abilities of the human colon cancer HT-29 cells,and its mechanism may be related to down-regulating the expression of bcl-2 protein and up-regulating the expressions of bax and Caspase-3 proteins,thus activating the apoptotic pathway of HT-29 cells.

Key words: colon cancer, Alkaline S.chinensis polysaccharides, invasive ability, apoptosis

中图分类号: 

  • R730.52