吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 379-382.doi: 10.13481/j.1671-587x.20150234

• 临床研究 • 上一篇    下一篇

肿瘤坏死因子α信号传导通路在儿童哮喘发病机制中的作用

齐玲1, 王玮瑶1, 张艳春2, 蔡尚霞2, 杨宁江1, 刘晓丽2   

  1. 1. 吉林医药学院病理学教研室, 吉林 吉林 132013;
    2. 北华大学第二附属医院儿科, 吉林 吉林 132021
  • 收稿日期:2014-08-28 出版日期:2015-03-28 发布日期:2015-04-04
  • 通讯作者: 杨宁江, 教授, 硕士研究生导师(Tel:0432-64560605, E-mail:1270lynj@sina.com);刘晓丽, 副主任医师(Tel:0432-63915536, E-mail:512493536@qq.com) E-mail:1270lynj@sina.com;512493536@qq.com
  • 作者简介:齐玲(1974-), 女, 吉林省吉林市人, 副教授, 医学博士, 主要从事凋亡通路方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(81201671);吉林省科技厅科研基金资助课题(20140414049GH);吉林省吉林市科技局科技计划项目资助课题(201339054)

Effects of TNF-α signal pathway in pathogenesis of asthma in children

QI Ling1, WANG Weiyao1, ZHANG Yanchun2, CAI Shangxia2, YANG Ningjiang1, LIU Xiaoli2   

  1. 1. Department of Pathology, Jilin Medical College, Jilin 132013, China;
    2. Department of Pediatrics, Second Affiliated Hospital, Beihua University, Jilin 132021, China
  • Received:2014-08-28 Online:2015-03-28 Published:2015-04-04

摘要:

目的:探讨哮喘儿童血清中肿瘤坏死因子α(TNF-α)信号传导通路相关蛋白在哮喘发病中的作用,为儿童哮喘的防治提供依据。方法:留取正常儿童(对照组,n=31)及临床缓解期、慢性持续期和急性发作期(哮喘组,n=33)哮喘患儿的血清。采用ELISA法分析血清中TNF-α、半胱氨酸蛋白酶8(Caspase-8)、肿瘤坏死因子相关受体2(TRAF-2)、受体相互作用蛋白(RIP)和半胱氨酸蛋白酶-3(Caspase-3)蛋白表达水平。结果:临床缓解期、慢性持续期和急性发作期哮喘患儿血清中TNF-α表达水平升高。与对照组比较,急性发作期哮喘患儿血清中TNF-α、Caspase-8和Caspase-3表达水平明显升高(P<0.05),临床缓解期哮喘患儿血清中Caspase-3蛋白表达水平降低(P<0.05),慢性持续期哮喘患儿血清中Caspase-3蛋白表达水平升高(P<0.05)。与对照组比较,哮喘组患儿血清中TRAF-2和RIP蛋白表达水平差异无统计学意义(P>0.05)。结论:TNF-α信号传导通路与儿童哮喘的发生有密切关联,其机制可能为激化TNF-α通路,使细胞中Caspase-3和Caspase-8表达水平增加,诱导哮喘。

关键词: 哮喘, 儿童, 肿瘤坏死因子α, 半胱氨酸蛋白酶8

Abstract:

Objective To explore the effects of tumor necrosis factor α(TNF-α) signal pathway related proteins in the serum of the asthma children in the pathogenesis of asthma,and to provide the basis for prevention and treatment of asthma in the children.Methods The serum of normal children(control group,n=31) and asthma children(asthma group,n=33) in clinical remission,chronic persistent and acute exacerbation periods were obtained. The expression levels of TNF-α,cysteine-containing aspirate-specific protease-8(Caspase-8),TNF receptor-associated factors-2 (TRAF-2),reportor interacting protein (RIP) and cysteine-containing aspirate-specific protease-3 (Caspase-3) proteins were detected by ELISA assay.Results The expression levels of TNF-α protein in serum of the asthma children in clinical remission group,chronic persistent group,and acute exacerbation group were increased. Compared with control group,the expression levels of TNF-α, Caspase-8,and Caspase-3 proteins in serum of the asthma children in acute exacerbation group were significantly increased(P<0.05);the expression level of Caspase-3 protein in serum of the asthma children in clinical remission group was significantly decreased(P<0.05);but the expression level of Caspase-3 protein in serum of the asthma children in chronic persistent group was significantly increased(P<0.05).There were no significant differences of the expression levels of TRAF2 and RIP proteins between asthma group and control group(P>0.05).Conclusion TNF-α signal pathway is closely related to the development of asthma in children,and its mechanism may be activating the TNF-α pathway to promote the expression levels of Caspase-3 and Caspase-8 in the cells and to induce asthma.

Key words: asthma, children, tumor necrosis factor-α, cysteine containing aspirate specific protease-8

中图分类号: 

  • R739.4