贝母瓜蒌散对慢性阻塞性肺疾病大鼠肺组织中血管紧张素Ⅱ表达水平的影响

doi:10.13481/j.1671-587x.20150318

摘要/Abstract

摘要：

目的:观察贝母瓜蒌散对慢性阻塞性肺疾病(COPD)大鼠肺组织中血管紧张素Ⅱ(AngⅡ)表达的影响,初步探讨贝母瓜蒌散对肺组织的保护作用及其机制。方法:将30只SD大鼠随机分为正常组、模型组和贝母瓜蒌散组,每组10只。正常组大鼠常规喂养,其余2组大鼠采用脂多糖(LPS)器官滴入加烟熏法,建立大鼠COPD模型。在造模第8天,正常组和模型组大鼠灌胃给予生理盐水(20 mL·kg^-1),贝母瓜蒌散组大鼠灌胃给予贝母瓜蒌散 (20 mL·kg^-1),每天1次,连续22 d。采用免疫组织化学法和免疫印记方法观察各组大鼠肺组织中AngⅡ表达情况。结果:与正常组比较,模型组大鼠出现了喘、咳嗽、气急、精神萎靡、吐痰流涎和毛发牙齿发黄等症状和体征。与正常组比较,模型组大鼠肺组织中AngⅡ的表达水平明显增加(P<0.05);与模型组比较,贝母瓜蒌散组大鼠肺组织中AngⅡ表达水平减少(P<0.05)。结论:贝母瓜蒌散能抵抗AngⅡ诱导的细胞炎症反应,明显改善COPD大鼠喘咳和气急等症状。

关键词: 贝母瓜蒌散, 慢性阻塞性肺疾病, 血管紧张素Ⅱ, 大鼠

Abstract:

Objective To investigate the effects of BeiMuGuaLou powder on the expression of angiotensin Ⅱ(AngⅡ) in thelung tissue of the rats with chronic obstructive pulmonary disease(COPD), and to study the protective effect of BeiMuGuaLou powder on lung tissue and its mechanism. Methods Thirty male SD rats were randomly divided into control group, COPD group and BeiMuGuaLou powder group, 10 rats in each group.The model of COPD was established by smoking combined with endotracheal injection of lipopolysaccharide(LPS) for 30 d.On the 8th day of modeling, the rats in control and COPD groups were lavaged with normal saline(20 mL·kg^-1).The rats in BeiMuGuaLou powder group were lavaged with BeiMuGuaLou powder(20 mL·kg^-1) for 22 d.The expression levels of AngⅡ in the lung tissue of the rats in various groups were determined by immunohistochemistry and Western blotting methods. Results Compared with control group, the rats in model group appeared dyspnea, cough, shortness of breath, apathia, spit saliva, yellow hair, yellow teeth, and other symptoms and signs.Compared with control group, the expression level of AngⅡ in lung tissue of the rats in model group was significantly increased(P<0.05);compared with model group, the expression level of AngⅡ in lung tissue of the rats in BeiMuGuaLou powder group was significantly reduced (P<0.05). Conclusion BeiMuGuaLou powder can resist the cell inflammatory reaction induced by AngⅡ, and obviously improve the asthma and cough, shortness of breath and other symptoms of the COPD rats.

Key words: BeiMuGuaLou powder, chronic obstructive pulmonary disease, angiotensin Ⅱ, rats

中图分类号:

R563

吴忠练, 黄学宽, 骆言, 张超男, 蒋娟. 贝母瓜蒌散对慢性阻塞性肺疾病大鼠肺组织中血管紧张素Ⅱ表达水平的影响[J]. 吉林大学学报(医学版), 2015, 41(03): 527-531.

WU Zhonglian, HUANG Xuekuan, LUO Yan, ZHANG Chaonan, JIANG Juan. Effects of BeiMuGuaLou powder on expression level of angiotensin Ⅱ in lung tissue of rats with chronic obstructive pulmonary disease[J]. Journal of Jilin University Medicine Edition, 2015, 41(03): 527-531.

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