吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (03): 573-577.doi: 10.13481/j.1671-587x.20150327

• 基础研究 • 上一篇    下一篇

5-HT7受体对帕金森病模型大鼠中缝背核5-HT能神经元电活动的调控作用

王爽1, 高捷2, 郭玉芳1, 王湘1   

  1. 1. 西安医学院病理生理学教研室 基础医学研究所, 陕西 西安 710021;
    2. 西安医学院管理学教研室, 陕西 西安 710021
  • 收稿日期:2014-10-13 发布日期:2015-08-01
  • 通讯作者: 王爽,副教授(Tel:029-86177414,E-mail:wangshuang78213@163.com) E-mail:wangshuang78213@163.com
  • 作者简介:王爽(1978-),男,陕西省西安市人,副教授,理学博士,主要从事神经退行性疾病的中枢机制研究。
  • 基金资助:

    陕西省科技厅科研基金资助课题(2012KJXX-43);教育部国家级大学生创新创业训练计划项目资助课题(201311840008);陕西省2013年省级大学生创新创业训练项目资助课题(1738)

Regulation effect of 5-HT7 receptor on electrical activity of 5-HT neurons in nucleus raphes dorsalis of rats with Parkinson's disease

WANG Shuang1, GAO Jie2, GUO Yufang1, WANG Xiang1   

  1. 1. Department of Pathophysiology, Institute of Basic Medical Sciences, Xi'an Medical University, Xi'an 710021, China;
    2. Department of Management, Xi'an Medical University, Xi'an 710021, China
  • Received:2014-10-13 Published:2015-08-01

摘要:

目的: 探讨5-羟色胺-7(5-HT7)受体对正常和帕金森病(PD)模型大鼠中缝背核(DRN)5-羟色胺(5-HT)能神经元电活动的调控作用,阐明5-HT7受体的性质及PD状态下该受体性状的改变。方法:28只雄性SD大鼠随机分为假手术组(n=12)和PD组(n=16)。PD组大鼠黑质致密部内注射6-OHDA,假手术组大鼠注射同等剂量生理盐水。2组大鼠静脉注射多个剂量(40~640 μg·kg-1,i.v.)的5-HT7受体激动剂AS19后,采用体细胞外电生理学记录观察大鼠DRN中5-HT能神经元放电频率的变化;静脉注射5-HT7受体拮抗剂SB269970后,观察PD组大鼠对激动剂和拮抗剂的敏感性,并与假手术组进行比较。结果:在假手术组中,与基础放电频率比较,5-HT7受体激动剂AS19(40~640 μg·kg-1,i.v.)能够明显增加大鼠5-HT能神经元的放电频率,放电频率增加到2.35±0.16(P<0.01);这种作用可以被SB269970(200 μg·kg-1,i.v.)完全反转,使大鼠5-HT能神经元的放电率恢复为0.37±0.03。在PD组中,相同剂量AS19(40~640 μg·kg-1,i.v.)对5-HT神经元的放电频率无兴奋效应(P=0.218)。结论:5-HT7受体对PD模型大鼠中缝背核5-HT能神经元的调控作用减弱。

关键词: 5-羟色胺-7受体, 中缝背核, 5-羟色胺能神经元, 电生理学

Abstract:

Objective To investigate the different effects of 5-hydroxytryptamine-7(5-HT7) on the electrical activities of 5-HT neurons in nucleus raphes dorsalis(DRN) of the normal and parkinson's disease(PD) rats, and to clarify the properties of 5-HT7 receptor and the changes in the PD rats. Methods 28 male SD rats were randomly divided into sham opration group (n=12) and PD group (n=16).6-OHDA was injected in the subtantia nigra pars compacta(SNc)of the rats in PD group, and the same dose of normal saline was injected in the SNc of the rats in sham opration group.The rats in two groups were injected with different doses of (40-640 μg·kg-1, i.v.) 5-HT7 receptor agonist AS19, and the changes of firing rates in 5-HT neurons of the rats were observed by extracellular electrophysiological recording in DRN.5-HT7 receptor antagonist SB269970 was injected to observe the sensitivities to agonist and the antagonist of the rats in PD group, and compared with sham operation group. Results Systemic administration of the selective 5-HT7 receptor agonist AS19(40-640 μg·kg-1, i.v.)increased the firing rate of 5-HT neurons of the rats in sham operation group, the mean firing rate of 5-HT neurons in the DRN was changed to 2.35±0.16(P<0.001), and completely reversed by the subsequent administration of SB269970(200 μg·kg-1, i.v.), the mean firing rate of 5-HT neurons in the DRN was changed to 0.37±0.03.The same dose of AS19 had no affection in the rats in PD group(P=0.218). Conclusion The regulation effect of 5-HT7 receptor on the 5-HT neurons in DRN of the PD rats is decreased.

Key words: 5-hydroxytryptamine-7 receptor, nucleus raphes dorsalis, 5-hydroxytryptamine neurons, electrophysiology

中图分类号: 

  • R338.2