丝胶对2型糖尿病大鼠肝脏组织中TNF-α和HNF-4α表达的影响

doi:10.13481/j.1671-587x.20150403

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (04): 685-689.doi: 10.13481/j.1671-587x.20150403

丝胶对2型糖尿病大鼠肝脏组织中TNF-α和HNF-4α表达的影响

刘东慧¹, 刘美晓¹, 王小杰², 张艳¹, 杨松鹤¹, 程露阳³, 陈志宏¹

1. 承德医学院人体解剖学教研室, 河北承德 067000;
2. 承德医学院基础医学研究所, 河北承德 067000;
3. 承德医学院免疫学教研室, 河北承德 067000

收稿日期:2015-01-20 发布日期:2015-08-01
通讯作者: 陈志宏,教授,硕士研究生导师(Tel:0314-2517018,E-mail:czh1971@126.com) E-mail:czh1971@126.com
作者简介:刘东慧(1987-),女,河北省廊坊市人,医学硕士,主要从事糖尿病及其并发症中医药防治方面的研究。
基金资助:
国家自然科学基金资助课题(81441133);河北省科技厅自然科学基金资助课题(H2013406096)

Effects of sericin on TNF-α and HNF-4α expressions in liver tissue of rats with type 2 diabetes mellitus

LIU Donghui¹, LIU Meixiao¹, WANG Xiaojie², ZHANG Yan¹, YANG Songhe¹, CHENG Luyang³, CHEN Zhihong¹

1. Department of Human Anatomy, Chengde Medical College, Chengde 067000, China;
2. Institute of Basic Medical Science, Chengde Medical College, Chengde 067000, China;
3. Department of Immmunology, Chengde Medical College, Chengde 067000, China

Received:2015-01-20 Published:2015-08-01

摘要/Abstract

摘要：

目的: 通过观察丝胶对2型糖尿病大鼠肝脏组织中肿瘤坏死因子α(TNF-α)和肝细胞核因子4α(HNF-4α)表达的影响,探讨丝胶治疗糖尿病的可能机制。方法: 36只雄性SD大鼠随机分为正常对照组、糖尿病模型组和丝胶治疗组,每组12只。链脲佐菌素(STZ)连续腹腔注射法制作2型糖尿病大鼠模型。待模型成功建立后,模型组大鼠不做任何处理,丝胶治疗组大鼠给予丝胶(2.4 g·kg^-1·d^-1)灌胃35 d。采用免疫组织化学SP法染色、蛋白免疫印迹法和RT-PCR法检测大鼠肝脏组织中TNF-α和HNF-4α的表达。结果: TNF-α蛋白免疫产物为棕黄色和棕褐色颗粒状,位于肝细胞的细胞浆;HNF-4α免疫阳性产物为棕褐色,位于肝细胞的细胞核。与正常对照组比较,糖尿病模型组大鼠肝脏组织中TNF-α、HNF-4α蛋白和mRNA的表达水平明显升高(P<0.01);与糖尿病模型组比较,丝胶治疗组大鼠肝脏TNF-α、HNF-4α蛋白和mRNA的表达水平明显降低(P<0.01)。结论: 丝胶可能通过下调肝脏组织中TNF-α和HNF-4α的表达保护糖尿病时肝脏损伤。

关键词: 丝胶, 糖尿病, 2型, 肝脏, 肿瘤坏死因子&alpha, 肝细胞核因子4&alpha

Abstract:

Objective To investigate the possible mechanisms of sericin in treating diabetes mellitus by observing the effects of sericin on the expressions of tumor necrosis factor-α (TNF-α) and hepatocyte nuclear factor 4 alpha (HNF-4α) in liver tissue of the rats with type 2 diabetes mellitus. Methods 36 male SD rats were randomly divided into normal control group,diabetes model group and sericin treatment group (n=12).The type 2 diabetes mellitus rat models were induced by continuously intraperitoneal injection of streptozotocin(STZ).After successfully establishing the diabetes models,the rats in diabetes model group received any more treatments,and the rats in sericin treatment group were lavaged with sericin (2.4 g·kg^-1·d^-1) for 35 d. SP immunohistochemical staining,Western blotting and RT-PCR were used to detect the TNF-α and HNF-4α expressions in liver tissue of the rats. Results The TNF-α protein immune positive products were tan and brown granules and located in the cytoplasm of liver cells;the HNF-4α immune positive products were tan and located in the nucleus of liver cells. Compared with normal control group,the TNF-α and HNF-4α protein and mRNA expression levels in liver tissue of the rats in diabetes model group were increased obviously (P<0.01).Compared with diabetes model group,the TNF-α and HNF-4α protein and mRNA expression levels in liver tissue of the rats in sericin treatment group were decreased obviously (P<0.01). Conclusion Sericin may protect the liver injury in the rats with diabetes mellitus by down-regulating the TNF-α and HNF-4α expressions in liver tissue.

Key words: sericin, diabetes mellitus,type 2, liver, tumor necrosis factor-α, hepatocyte nuclear factor 4 alpha

中图分类号:

R587.1

刘东慧, 刘美晓, 王小杰, 张艳, 杨松鹤, 程露阳, 陈志宏. 丝胶对2型糖尿病大鼠肝脏组织中TNF-α和HNF-4α表达的影响[J]. 吉林大学学报(医学版), 2015, 41(04): 685-689.

LIU Donghui, LIU Meixiao, WANG Xiaojie, ZHANG Yan, YANG Songhe, CHENG Luyang, CHEN Zhihong. Effects of sericin on TNF-α and HNF-4α expressions in liver tissue of rats with type 2 diabetes mellitus[J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 685-689.

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丝胶对2型糖尿病大鼠肝脏组织中TNF-α和HNF-4α表达的影响

Effects of sericin on TNF-α and HNF-4α expressions in liver tissue of rats with type 2 diabetes mellitus

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