纳米二氧化硅颗粒对HL-7702细胞黏附和迁移能力的影响

doi:10.13481/j.1671-587x.20150412

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (04): 732-736.doi: 10.13481/j.1671-587x.20150412

纳米二氧化硅颗粒对HL-7702细胞黏附和迁移能力的影响

潘涛^1,2, 郭彩霞³, 金明华¹, 刘晓梅¹, 刘颖¹, 杜海英¹, 孙志伟^1,4

1. 吉林大学公共卫生学院卫生毒理学教研室, 吉林长春 130021;
2. 西安医学院公共卫生系, 陕西西安 710021;
3. 首都医科大学公共卫生学院劳动卫生与环境卫生学系, 北京 100069;
4. 首都医科大学公共卫生学院卫生化学与毒理学系, 北京 100069

收稿日期:2014-10-06 发布日期:2015-08-01
通讯作者: 孙志伟,教授,博士研究生导师(Tel:010-83911507,E-mail:zwsun@hotmail.com) E-mail:zwsun@hotmail.com
作者简介:潘涛(1984-),男,山东省日照市人,医学硕士,主要从事环境毒理学方面的研究。
基金资助:
国家自然科学基金资助课题(81172704)

Effects of SiO₂ nanoparticles on cell adhesion and migration of HL-7702 cells

PAN Tao^1,2, GUO Caixia³, JIN Minghua¹, LIU Xiaomei¹, LIU Ying¹, DU Haiying¹, SUN Zhiwei^1,4

1. Department of Health Toxicology, School of Public Health, Jilin University, Changchun 130021, China;
2. Department of Public Health, Xi'an Medical University, Xi'an 710021, China;
3. Department of Occupational and Environmental Health, School of Public Health, Capital Medical University, Beijing 100069, China;
4. Department of Sanitary Chemistry and Toxicology, School of Public Health, Capital Medical University, Beijing 100069, China

Received:2014-10-06 Published:2015-08-01

摘要/Abstract

摘要：

目的: 探讨纳米二氧化硅颗粒对HL-7702细胞黏附迁移能力的影响,为其安全性评价提供实验依据。方法: 体外培养的人正常肝细胞株HL-7702随机分为对照组和纳米二氧化硅颗粒暴露组,暴露浓度分别为 12.5、25.0、50.0和100.0 mg·L^-1。采用透射电子显微镜(TEM)及动态光散射粒度分析仪(DLS)对纳米二氧化硅颗粒进行观察。细胞处理 24 h 后,细胞黏附实验检测细胞黏附能力;划痕修复实验检测细胞迁移能力,TEM观察细胞对纳米二氧化硅颗粒的摄取及颗粒在细胞内的分布。结果: TEM观察,纳米二氧化硅呈圆形,颗粒大小均匀一致,分散性良好,颗粒平均粒径为(67.42±5.69) nm。DLS法检测,纳米二氧化硅颗粒在无血清RPMI-1640培养液中水合粒径为(134.13±2.78) nm,而在含1%、5% 和10%血清的RPMI-1640培养液中的水合粒径明显增大。细胞黏附实验及划痕修复实验,与对照组比较,在无毒剂量下,纳米二氧化硅颗粒暴露组细胞的相对黏附率和损伤修复率明显下调(P<0.05),且随着作用浓度的升高下调作用愈加明显。与对照组比较,在无毒剂量作用下,纳米二氧化硅暴露组大量的纳米二氧化硅颗粒已被细胞摄取,颗粒主要以成簇的形式分布于内吞小泡中或散在分布于胞质中,可见细胞膜凹陷、包裹吞噬纳米颗粒的过程。结论: 纳米二氧化硅颗粒能够抑制HL-7702细胞的黏附和迁移能力,并呈一定的浓度依赖效应。

关键词: 纳米二氧化硅颗粒, HL-7702细胞, 细胞黏附, 细胞迁移, 细胞摄取

Abstract:

Objective To study the effects of SiO₂ nanoparticles (SiO₂ NPs) on the cell adhesion and migration of HL-7702 cells, and to provide experimental basis for safety evaluation of SiO₂ NPs. Methods The in vitro cultured human normal liver cell line HL-7702 were randomly divided into control and SiO₂ NPs exposure groups at the concentrations of 12.5,25.0,50.0,and 100.0 mg·L^-1,respectively.Transmission electron microscope (TEM) and dynamic light scattering (DLS) method were used to observe the characteristics of SiO₂ NPs.After 24 h exposure,cell adhesion assay was applied to examine the changes of cell adherence;wound healing assay was used to discover the changes of cell migration;the cellular uptake and biodistribution of SiO₂ NPs were observed using TEM. Results Based on the result of TEM,SiO₂ NPs were spherically shaped,uniformly sized and sporadically dispersed with the diameter of (67.42±5.69) nm. The results of DLS method showed that the average hydrated particle size of SiO₂ NPs dispersing in RPMI-1640 serum-free medium was (134.13±2.78) nm,while the hydrated particle sizes of SiO₂ NPs dispersing in RPMI-1640 with 1%,5%,or 10% serum were apparently enlarged.The results of cell adhesion and wound healing assays showed that the adhesion rates and the healing rates of cells could be down-regulated by SiO₂ NPs even under a dose that did not affect the cell viability and membrane integrity(P<0.05),furthermore,the down regulation effect was significantiy increased along with the enlargement of the SiO₂ NPs' concentration. The TEM results showed that after exposed to nontoxic dose of SiO₂ NPs,the cells accumulated a large number of nanoparticles,the particles mostly dispersed in endocytic recycling compartment,and some were in the cytoplasm;some parts of the cell membrans were caved,and the procedure of endocytose could be captured. Conclusion SiO₂ NPs could inhibit the cell adhesion and migration in a dose-dependent manner.

Key words: SiO₂ nanoparticles, HL-7702 cells, cell adhesion, cell migration, cellular uptake

中图分类号:

R992

潘涛, 郭彩霞, 金明华, 刘晓梅, 刘颖, 杜海英, 孙志伟. 纳米二氧化硅颗粒对HL-7702细胞黏附和迁移能力的影响[J]. 吉林大学学报(医学版), 2015, 41(04): 732-736.

PAN Tao, GUO Caixia, JIN Minghua, LIU Xiaomei, LIU Ying, DU Haiying, SUN Zhiwei. Effects of SiO₂ nanoparticles on cell adhesion and migration of HL-7702 cells[J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 732-736.

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纳米二氧化硅颗粒对HL-7702细胞黏附和迁移能力的影响

Effects of SiO₂ nanoparticles on cell adhesion and migration of HL-7702 cells

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纳米二氧化硅颗粒对HL-7702细胞黏附和迁移能力的影响

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Effects of SiO₂ nanoparticles on cell adhesion and migration of HL-7702 cells