S100钙结合蛋白P在胃癌患者胃癌组织和血清中的表达及其临床意义

doi:10.13481/j.1671-587x.20150431

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (04): 830-835.doi: 10.13481/j.1671-587x.20150431

S100钙结合蛋白P在胃癌患者胃癌组织和血清中的表达及其临床意义

乔晓娟¹, 苏秀兰², 师永红³, 杜华³, 张树才⁴, 李云霞¹

1. 内蒙古医科大学附属医院保健中心, 内蒙古呼和浩特 010050;
2. 内蒙古医科大学附属医院临床医学研究中心, 内蒙古呼和浩特 010050;
3. 内蒙古医科大学附属医院病理科, 内蒙古呼和浩特 010050;
4. 首都医科大学附属北京胸科医院北京市结核病胸部肿瘤研究所肿瘤内科, 北京 101149

收稿日期:2014-12-10 发布日期:2015-08-01
通讯作者: 张树才,主任医师,博士研究生导师(Tel:010-89509304,E-mail:sczhang6304@163.com);李云霞,主任医师,硕士研究生导师(Tel:0471-6636432,E-mail:yunxia728@163.com) E-mail:sczhang6304@163.comyunxia728@163.com
作者简介:乔晓娟(1981-),女,内蒙古自治区锡林郭勒盟人,主治医师,在读医学博士,主要从事恶性肿瘤内科治疗方面的研究。
基金资助:
内蒙古自治区高等学校科学研究项目资助课题(NJZY12148)

Expressions of S100 calcium-binding protein P in gastric cancer tissue and serum of patients with gastric cancer and their clinical significances

QIAO Xiaojuan¹, SU Xiulan², SHI Yonghong³, DU Hua³, ZHANG Shucai⁴, LI Yunxia¹

1. Health Care Center, Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010050, China;
2. Clinical Medical Research Center, Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010050, China;
3. Department of Pathology, Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010050, China;
4. Department of Medical Oncology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China

Received:2014-12-10 Published:2015-08-01

摘要/Abstract

摘要：

目的: 研究胃癌患者胃癌组织和血清中S100钙结合蛋白P(S100P)表达及其与临床病理参数的关系,监测原发肿瘤切除前后血清S100P蛋白水平,探讨其与预后的关系。 方法: 选取手术切除并经病理检查确诊的胃癌患者50例作为研究对象,所有患者均可获取术后石蜡组织标本及术前血清,其中20例患者可获取新鲜胃癌组织及配对癌旁组织,另30例患者可获取术后动态血清。半定量RT-PCR法检测组织中S100PmRNA水平;免疫组织化学法及ELISA法分别检测组织和血清中S100P蛋白水平。选择同期30名体检者为正常对照组,ELISA法检测血清S100P蛋白水平。结果: 胃癌组织S100P mRNA表达水平明显低于癌旁组织(P<0.05);胃癌组织S100P蛋白阳性表达率低于癌旁组织(P<0.05),S100P表达下调与脉管癌栓有关联(P<0.05)。胃癌患者术前血清S100P水平低于正常对照组,但差异无统计学意义(P>0.05);无淋巴结转移胃癌患者血清S100P水平高于有淋巴结转移患者,但差异无统计学意义(P> 0.05);胃癌患者手术后2周血清S100P水平明显低于手术前(P<0.05)。结论: S100P在胃癌患者胃癌组织和血清中表达水平下调,其表达水平与预后有关联。持续动态监测血清S100P水平变化可能预测复发及判断预后。

关键词: S100钙结合蛋白P, 胃肿瘤, 预后

Abstract:

Objective To investigate the relationship between the expressions of S100 calcium-binding protein P (S100P) in gastric cancer tissue and serum of the patients with gastric cancer and their clinicopathological parameters and monitor the serum S100P protein levels before and after tumor resection,and to explore its correlation with the prognosis of gastric cancer. Methods 50 patients with gastric cancer confirmed by surgical resection and pathological examination were selected.The pre-operative serum and post-operative gastric cancer tissue paraffin samples were collected.The fresh tissue samples and adjacent tissue were adopted from 20 patients with gastric cancer,and the postoperative dynamic serum samples were obtained from the other 30 patients with gastric cancer.Meanwhile,30 health volunteers were selected as normal control group.The expression level of S100P mRNA in gastric cancer tissue was detected by semi-quantitative RT-PCR.Immunohistochemistry and enzyme linked immunosorbent assay (ELISA) were used to detect the expression levels of S100P protein both in gastric cancer tissue and serum. Results The expression level of S100P mRNA in gastric cancer tissue was significantly lower than that in adjacent normal tissue (P<0.05);the positive expression rate of S100P protein in gastric cancer tissue was significantly lower than that in adjacent tissue (P<0.05);the down-regulation of the expression of S100P protein in gastric cancer tissue was positively correlated with vascular cancer embolus (P<0.05).The serum S100P level in the patients with gastric cancer before operation was lower than that in normal control group,but there was no significant difference (P> 0.05).The serum S100P level in the gastric cancer patients without lymph node metastasis was higher than that in the patients with lymph node metastasis,but there was no significant difference (P> 0.05).The serum S100P level in the patients with gastric cancer 2 weeks after operation was significantly lower than before operation. Conclusion The expression levels of S100P in gastric cancer tissue and serum of the patients with gastric cancer are decreased,and its expression level is correlated with the prognosis. The continuous dynamic monitoring of serum S100P level may predict the recurrence and prognosis.

Key words: S100 calcium-binding protein P, stomachneoplasms, prognosis

中图分类号:

R735.2

乔晓娟, 苏秀兰, 师永红, 杜华, 张树才, 李云霞. S100钙结合蛋白P在胃癌患者胃癌组织和血清中的表达及其临床意义[J]. 吉林大学学报(医学版), 2015, 41(04): 830-835.

QIAO Xiaojuan, SU Xiulan, SHI Yonghong, DU Hua, ZHANG Shucai, LI Yunxia. Expressions of S100 calcium-binding protein P in gastric cancer tissue and serum of patients with gastric cancer and their clinical significances[J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 830-835.

参考文献

[1] Wang Q,Zhang YN,Lin GL,et al.S100P,a potential novel prognostic marker in colorectal cancer[J].Oncol Rep,2012,28(1):303-310.

[2] Maciejczyk A,Lacko A,Ekiert M,et al.Elevated nuclear S100P expression is associated with poor survival in early breast cancer patients[J].Histol Histopathol,2013,28(4):513-524.

[3] Yuan RH,Chang KT,Chen YL,et al.S100P expression is a novel prognostic factor in hepatocellular carcinoma and predicts survival in patients with high tumor stage or early recurrent tumors[J].PLoS One,2013,8(6):e65501.

[4] Guo L,Chen S,Jiang H,et al.The expression of S100P increases and promotes cellular proliferation by increasing nuclear translocation of beta-catenin in endometrial cancer[J].Int J Clin Exp Pathol,2014,7(5):2102-2112.

[5] Jia SQ,Niu ZJ,Zhang LH,et al.Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics[J].J Cancer Res Clin Oncol,2009,135(3):403-411.

[6] 贾淑芹,季加孚,苏秀兰.S100P 在胃癌中的下调表达及意义[J].肿瘤防治研究,2011,4(38):423-426.

[7] 乔广超.S100A4、S100A9和S100P在胃癌组织中的表达及临床意义[D].泰安:泰山医学院,2012.

[8] Ge F,Wang C,Wang W,et al.S100P predicts prognosis and drug resistance in gastric cancer[J].Int J Biol Markers,2013,28(4):e387-e392.

[9] Arumugam T,Simeone DM,Schmidt AM,et al.S100P stimulates cell proliferation and survival via receptor for activated glycation end products (RAGE)[J].J Biol Chem,2004,279(7):5059-5065.

[10] Donato R,Cannon BR,Sorci G,et al.Functions of S100 proteins[J].Curr Mol Med,2013,13(1):24-57.

[11] Becker T,Gerke V,Kube E,et al.S100P,a novel Ca²⁺-binding protein from human placenta.cDNA cloning,recombinant protein expression and Ca²⁺ binding properties[J].Eur J Biochem,1992,207(2):541-547.

[12] Parkkila S,Pan PW,Ward A,et al.The calcium-binding protein S100P in normal and malignant human tissues[J].BMC Clin Pathol,2008,8:2.

[13] Austermann J,Nazmi AR,Muller-Tidow C,et al.Characterization of the Ca²⁺ -regulated ezrin-S100P interaction and its role in tumor cell migration[J].J Biol Chem,2008,283(43):29331-29340.

[14] Arumugam T,Ramachandran V,Gomez SB,et al.S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis[J].Clin Cancer Res,2012,18(16):4356-4364.

[15] Zhao XM,Bai ZG,Ma XM,et al.Impact of S100P expression on clinical outcomes of gastric cancer patients with adjuvant chemotherapy of oxaliplatin and its mechanisms[J].Zhonghua Wai Ke Za Zhi,2010,48(13):1004-1008.

[16] Zhao XM,Bai ZG,Wu P,et al.S100P enhances the chemosensitivity of human gastric cancer cell lines[J].Cancer Biomark,2013,13(1):1-10.

[17] Zhang Q,Hu H,Shi X,et al.Knockdown of S100P by lentiviral-mediated RNAi promotes apoptosis and suppresses the colony-formation ability of gastric cancer cells[J].Oncol Rep,2014,31(5):2344-2350.

[18] Du CY,Chen JG,Zhou Y,et al.Impact of lymphatic and/or blood vessel invasion in stage Ⅱ gastric cancer[J].World J Gastroenterol,2012,18(27):3610-3616.

S100钙结合蛋白P在胃癌患者胃癌组织和血清中的表达及其临床意义

Expressions of S100 calcium-binding protein P in gastric cancer tissue and serum of patients with gastric cancer and their clinical significances

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	赵丹, 曹东慧, 金美善, 吴孟辉, 王玥琦, 杨娜, 周天宇, 张厚君, 姜晶, 曹雪源. β-甘草次酸对炎症相关胃癌的抑制作用及其机制[J]. 吉林大学学报(医学版), 2018, 44(06): 1150-1155.
[2]	马立聪, 高芳, 董文杰, 田旭阳, 党彤, 贾彦彬. ITGA1基因多态性与幽门螺杆菌感染的关联性分析[J]. 吉林大学学报(医学版), 2018, 44(06): 1275-1279.
[3]	朱广伟, 郑炜, 黄永建, 华进, 杨树钢, 叶建新. 髓样分化因子88在结直肠癌患者癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1047-1051.
[4]	朱涛, 张国明, 严飞, 刘正, 霍强. 急性心肌梗死患者PCI术后心率变异性对主要不良心脏事件的评估价值[J]. 吉林大学学报(医学版), 2018, 44(04): 780-785.
[5]	高欢, 白元松, 代恩勇, 韩冷, 卢振霞, 赵亚男. 复发/难治性儿童B淋巴母细胞淋巴瘤1例报告及文献复习[J]. 吉林大学学报(医学版), 2018, 44(02): 416-420.
[6]	于云鹤, 杜烨, 韩冰, 李嗣杰, 宋乐连, 范志民. 新辅助化疗对Luminal B型乳腺癌各亚组的疗效观察及预后分析[J]. 吉林大学学报(医学版), 2018, 44(02): 356-362.
[7]	范励雯, 付彤, 古林, 吴迪, 贾泓瑶, 宋东. 副乳腺癌1例报告及文献复习[J]. 吉林大学学报(医学版), 2018, 44(02): 412-415.
[8]	何欣, 杨婷婷, 高阳阳, 宋艳秋. 乳腺癌患者心理韧性及其心理干预方式的研究进展[J]. 吉林大学学报(医学版), 2018, 44(02): 455-460.
[9]	杨冬野, 焦洋, 檀碧波, 杜志坚, 胡晓杰, 刘飞. Gli1基因在胃癌组织中的表达及其对胃癌细胞生物学特性的影响[J]. 吉林大学学报(医学版), 2018, 44(01): 63-67.
[10]	刘刚, 赵民学, 王德盛. 血清胃蛋白酶原、胃泌素17和幽门螺杆菌抗体检测在胃癌筛查中的应用[J]. 吉林大学学报(医学版), 2017, 43(06): 1182-1185.
[11]	孙慧颖, 杜波, 禹智, 刘洪泉, 于红. 突发性聋伴良性阵发性位置性眩晕病例分析并文献复习[J]. 吉林大学学报(医学版), 2017, 43(05): 1025-1029.
[12]	文思敏, 于多, 吕欣, 王铁君. 紫杉醇联合顺铂与单药顺铂2种同步放化疗方案对中晚期宫颈癌患者预后的影响及安全性评价[J]. 吉林大学学报(医学版), 2017, 43(05): 1002-1008.
[13]	苏军燕, 李迎今, 徐长妍. 急性白血病患儿白血病干细胞表面CD96和CD71的表达对其疗效和预后的影响[J]. 吉林大学学报(医学版), 2017, 43(04): 776-781.
[14]	杨冬阳, 赖晓嵘, 黎莹, 马立宇, 罗刚, 李子俊, 徐飞, 马冬. Ⅳ期结直肠癌轻微症状患者原发灶切除和KRAS基因突变对其预后的影响[J]. 吉林大学学报(医学版), 2017, 43(04): 805-811.
[15]	程诗佳, 王旭, 崔久嵬. 肿瘤患者异常铁代谢及其在肿瘤诊断和治疗中应用的研究进展[J]. 吉林大学学报(医学版), 2017, 43(01): 200-204.