键合靶向纳米胶束对脑胶质瘤细胞的组织相容性和靶向性

doi:10.13481/j.1671-587x.20150607

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (06): 1134-1138.doi: 10.13481/j.1671-587x.20150607

键合靶向纳米胶束对脑胶质瘤细胞的组织相容性和靶向性

韩海玲^1,2, 苗壮³, 岳军⁴, 赵长福², 景遐斌⁴, 金顺子¹, 王占峰^2,3

1. 吉林大学公共卫生学院卫生部放射生物学重点实验室, 吉林长春 130021;
2. 北华大学附属医院, 吉林吉林 132011;
3. 吉林大学中日联谊医院神经外2科, 吉林长春 130033;
4. 中国科学院长春应用化学研究所高分子物理与化学国家重点实验室, 吉林长春 130022

收稿日期:2015-05-05 发布日期:2016-01-11
通讯作者: 王占峰,副主任医师,硕士研究生导师(Tel:0431-84995718,E-mail:1206hhl@sina.com) E-mail:1206hhl@sina.com
作者简介:韩海玲(1977-),女,吉林省松原市人,副主任医师,在读医学博士,主要从事脑血管病的生物治疗和基础方面的研究。
基金资助:
国家自然科学基金资助课题(20904002);教育部新世纪优秀人才计划项目资助课题(NCET-10-0171);吉林省科技厅青年基金资助课题(20130522020JH);吉林省高技术产业发展专项资助课题(JF2012C005-2);吉林省教育厅十一五重大课题(2009139);吉林大学白求恩医学科研支持计划项目资助课题(2013107027)

Biocompatibility and brain-targeting ability of nano-micelles on glioma cells

HAN Hailing^1,2, MIAO Zhuang³, YUE Jun⁴, ZHAO Changfu², JING Xiabin⁴, JIN Sunzi¹, WANG Zhanfeng^2,3

1. Key Laboratory of Radiobiology, Ministry of Health School of Public Health, Jilin University, Changchun 130021, China;
2. Affliatted Hospital, Beihua University, Jilin 132011, China;
3. Department of Neurosurgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China;
4. State Key Lab of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, China

Received:2015-05-05 Published:2016-01-11

摘要/Abstract

摘要：

目的:探讨转铁蛋白受体的单克隆抗体(OX26)与聚乳酸聚乙二醇(PEG-PLA)键合靶向纳米胶束(copolymer/OX26)的生物相容性和安全性,阐明其作为脑胶质瘤靶向治疗载体的可行性。方法:体外培养C6胶质瘤细胞,用不同浓度(10.0、20.0、40.0和80.0 mg·L^-1)纳米胶束作为实验组,不含胶束的培养液作为对照组;台盼蓝染色法检测靶向纳米胶束对胶质瘤C6细胞的增殖抑制作用;流式细胞术检测靶向纳米胶束作用后C6胶质瘤细胞凋亡和细胞周期变化;激光共聚焦显微镜观察肿瘤细胞对靶向胶束的摄入情况。结果:与对照组比较,OX26靶向纳米胶束作用后C6细胞数无明显改变(P>0.05), 各组细胞凋亡率和细胞周期细胞比例差异亦无统计学意义(P>0.05);靶向纳米胶束组细胞内的荧光强度明显高于空白胶束组和空白对照组(P<0.05),并且这种作用随药物作用时间和浓度的增加而增加。结论:OX26靶向纳米胶束对C6细胞增殖和凋亡无影响,键合OX26提高了C6细胞对胶束的摄入量。

关键词: 神经胶质瘤, 靶向纳米胶束, 转铁蛋白受体, 组织相容性

Abstract:

Objective To investigate thebiocompatibility and safety of nanoscale brain-targeting-carrier micelles[poly(ethylene)-b-poly(lactic acid) /OX26 conjugate micells(copolymer/OX26)],and to explore its possibility as brain-targeted-drug carrier for brain glioma. Methods The C6 glioma cells were cultured in vitro and divided into experimental groups with different concentrations (10,20,40,80 mg·L^-1) of nano micelle,and the medium without micelle was used as control group.The inhibitory effect of nano-micelles on the rat brain glioma C6 cells was examined by Trypan blue cell counting assay.Flow cytometry (FCM) was used to detect the changes of apoptosis and cell cycle of C6 cells,and confocal laser scanning microscope (CLSM) was performed to analyze the distribution of copolymer/OX26 into C6 cells. Results The results of Trypan blue cell counting assay showed copolymer/OX26 didn't affect the growth of C6 cells,and there were no significant differences in the number of C6 cells between control group and expreimental groups(P>0.05).The results of FCM showed that the cell cycle and and the apoptotic rates of C6 cells had no changes compared with control group(P>0.05).The results of CLSM showed that the fluorescence intensities in experimental groups were higher than those in blank micelles group and blank control group(P<0.05),and they were increased in dose-and time-dependent manner(P<0.05). Conclusion Copolymer/OX26 has no effect on the growth and apoptosis of glioma cells.By bonding OX26, copolymer/OX26 can significantly increase the intake of C6 cells on the nano micelles.

Key words: glioma, brain-targeting-carrier micelles, transferrin receptor, histocompatibility

中图分类号:

R739.4

韩海玲, 苗壮, 岳军, 赵长福, 景遐斌, 金顺子, 王占峰. 键合靶向纳米胶束对脑胶质瘤细胞的组织相容性和靶向性[J]. 吉林大学学报(医学版), 2015, 41(06): 1134-1138.

HAN Hailing, MIAO Zhuang, YUE Jun, ZHAO Changfu, JING Xiabin, JIN Sunzi, WANG Zhanfeng. Biocompatibility and brain-targeting ability of nano-micelles on glioma cells[J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1134-1138.

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键合靶向纳米胶束对脑胶质瘤细胞的组织相容性和靶向性

Biocompatibility and brain-targeting ability of nano-micelles on glioma cells

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