血清ADA水平和T-spot.TB联合检测在肺结核诊断中的应用

doi:10.13481/j.1671-587x.20160222

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (02): 306-310.doi: 10.13481/j.1671-587x.20160222

血清ADA水平和T-spot.TB联合检测在肺结核诊断中的应用

冯晓朦, 王晶莹, 时景伟, 李威, 高申, 冯雪飞, 谢风

吉林大学中日联谊医院检验科, 吉林长春 130033

收稿日期:2015-11-09 发布日期:2016-03-31
通讯作者: 谢风,教授,硕士研究生导师(Tel:0431-84995455,E-mail:xiefeng11@126.com) E-mail:xiefeng11@126.com
作者简介:冯晓朦(1990-),女,吉林省吉林市人,在读医学硕士,主要从事临床实验室检验学方面的研究。
基金资助:
国家"863"计划资助子项目资助课题(20141422010)

Application of combined detection of serum levels of ADA and T-spot.TB in diagnosis of pulmonary tuberculosis

FENG Xiaomeng, WANG Jiingying, SHI Jingwei, LI Wei, GAO Shen, FENG Xuefei, XIE Feng

Deparment of Clinical Laboratory, China-Japan Union Hospital, Jilin University, Changchun 130033, China

Received:2015-11-09 Published:2016-03-31

摘要/Abstract

摘要：

目的: 联合应用血清腺苷脱氨酶(ADA)水平检测和结核感染T细胞斑点试验(T-spot.TB),探讨其对肺结核诊断的临床价值。方法: 选取本院拟诊为肺结核患者159例,分为肺结核组(n=91)和非肺结核组(n=68),随机选取健康体检者80名作为健康对照组,检测各组受试者血清ADA水平和T-spot.TB斑点数,比较各组ADA水平和T-spot.TB的阳性率及其对肺结核诊断的灵敏性与特异性。结果: 肺结核组、非肺结核组和健康对照组受试者血清ADA水平分别为(22.10±6.60)、(16.90±6.35)和(8.70±5.98)U·L^-1,肺结核组患者血清ADA水平明显高于非肺结核组和健康对照组(P<0.05)。血清ADA水平对肺结核诊断的阳性率为56.0%,T-spot.TB对肺结核诊断的阳性率为87.9%。采用平行联合试验,检测灵敏性为91.2%;采用系列联合试验,特异性为94.6%。结论: 联合检测血清ADA水平及T-spot.TB斑点数能够显著提高肺结核临床诊断。

关键词: 肺结核, 腺苷的脱氨酶, 结核感染T细胞斑点试验

Abstract:

Objective: To combine the detection of serum levels of adenosine deaminase(ADA)and T-cell spot test(T-spot.TB), and to explore their significances in diagnosis of pulmonary tuberculosis.Methods: 159 patients suspected with pulmonary tuberculosis were selected and divided into pulmonary tuberculosis group and non-tuberculosis group(n=68); 80 healthy people were randonly selected as healthy control group. The serum ADA levels and number of T-spot of the subjects in three groups were detected. Ther serum ADA levels and the positive rates of T-spot.TB in various groups and their sensitivities and specifities were compared.Results: The serum ADA level of the patients in pulmonary tuberculosis gruop was (22.10±6.60)U·L^-1;those in non-tuberculosis group and healthy control group were (16.90±6.35) and (8.70±5.98)U·L^-1;the serum ADA level in pulmonary tuberculosis group was significantly higher than those in non-tuberculosis group and heathy control group(P<0.05).The positive rate of serum ADA level in diagnosis of pulmonary tuberculosis was 56% and the T-spot.TB positive rate in diagnosis of pulmonary tuberculosis was 87.9%.Combined use of parallel test, the detection sensitivity was 91.2%; using the series of joint tests, the specificity was 94.6%.Conclusion: Combined detection of serum level of ADA and T-spot.TB can significantly improve the clinical diagnosis efficacy of pulmonary tuberculosis.

Key words: pulmonary tuberculosis, adenosine deaminase, T-spot.TB

中图分类号:

冯晓朦, 王晶莹, 时景伟, 李威, 高申, 冯雪飞, 谢风. 血清ADA水平和T-spot.TB联合检测在肺结核诊断中的应用[J]. 吉林大学学报(医学版), 2016, 42(02): 306-310.

FENG Xiaomeng, WANG Jiingying, SHI Jingwei, LI Wei, GAO Shen, FENG Xuefei, XIE Feng. Application of combined detection of serum levels of ADA and T-spot.TB in diagnosis of pulmonary tuberculosis[J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 306-310.

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血清ADA水平和T-spot.TB联合检测在肺结核诊断中的应用

Application of combined detection of serum levels of ADA and T-spot.TB in diagnosis of pulmonary tuberculosis

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