吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 711-715.doi: 10.13481/j.1671-587x.20160415

• 基础研究 • 上一篇    下一篇

五味子甲素对脑胶质瘤C6细胞生长的抑制作用及其机制

陈雪1, 张玉影2, 邵玉2, 张璐妮2, 宁明杰2, 唐英2, 齐玲2, 李蕴潜3   

  1. 1. 吉林医药学院实验中心, 吉林 吉林 132013;
    2. 吉林医药学院病理教研室, 吉林 吉林 132013;
    3. 吉林大学第一医院神经外科, 吉林 长春 130021
  • 收稿日期:2015-10-14 发布日期:2016-07-20
  • 通讯作者: 齐玲,副教授,硕士研究生导师(Tel:0432-64560027,E-mail:qiling1718@163.com);李蕴潜,副教授,硕士研究生导师(Tel:0431-85933066,E-mail:yunqianli@gmail.com) E-mail:qiling1718@163.com;yunqianli@gmail.com
  • 作者简介:陈雪(1981-),女,吉林省吉林市人,实验师,医学硕士,主要从事抗肿瘤药物方面的研究。
  • 基金资助:

    吉林省科技厅科研项目资助课题(20140414049GH,20140101114JC);吉林省教育厅科研项目资助课题(2016236);吉林省卫计委科研项目资助课题(2014Z104)

Inhibitory effect of deoxyschizandrin on growth of brain glioma cells and its mechanism

CHEN Xue1, ZHANG Yuying2, SHAO Yu2, ZHANG Luni2, NING Mingjie2, TANG Ying2, QI Ling2, LI Yunqian3   

  1. 1. Experiment Center, Jilin Medical University, Jilin 132013, China;
    2. Department of Pathology, Jilin Medical University, Jilin 132013, China;
    3. Department of Neurosurgery, First Hospital, Jilin University, Changchun 130021, China
  • Received:2015-10-14 Published:2016-07-20

摘要:

目的:探讨五味子甲素对脑胶质瘤C6细胞生长的抑制作用,阐明其作用机制。方法:培养大鼠脑胶质瘤C6细胞,将其分为对照组及50、100和200mg·L-1五味子甲素组,采用MTT法检测C6细胞增殖率,流式细胞术分析细胞周期百分率,酶联免疫吸附实验(ELISA)检测细胞培养上清中CyclinD1、Bax、Bcl-2和Casepase-3蛋白表达水平。结果:与对照组比较,24和48h时50、100和200 mg·L-1五味子甲素组细胞增殖率均明显降低(P < 0.01),72h时100和200 mg·L-1五味子甲素组细胞增殖率明显降低(P < 0.05或P < 0.01)。与对照组比较,200 mg·L-1五味子甲素组SubG1期细胞百分率升高(P < 0.05),S期细胞百分率降低(P < 0.05)。与对照组比较,100和200mg·L-1五味子甲素组细胞CyclinD1蛋白表达水平降低(P < 0.01),不同浓度五味子甲素组Bax蛋白表达水平升高(P < 0.05或P < 0.01),200mg·L-1五味子甲素组Bcl-2蛋白表达水平降低(P < 0.01),不同浓度五味子甲素组Bax/Bcl-2比值明显升高(P < 0.01),200 mg·L-1五味子甲素组Caspase-3蛋白表达水平升高(P < 0.01)。结论:五味子甲素通过下调CyclinD1蛋白表达水平、上调促凋亡因子Bax和Bcl-2表达水平而抑制C6细胞的生长。

关键词: 五味子甲素, 胶质瘤细胞, CyclinD1, Bax, Bcl-2

Abstract:

Objective: To study the inhibitory effect of deoxyschizandrin on the growth of brain glioma C6 cells,and to explore its mechanism. Methods: The rat glioma C6 cells were cultured and divided into control group,50,100,and 200 mg·L-1 deoxyschizandrin groups. The proliferation rates of C6 cells were examined by MTT assay;the changes of cell cycles were examined by flow cytometry;the expression levels of CyclinD1,Bax,Bcl-2 and Caspase-3 proteins in supernant were detected by ELISA assay. Results: Compared with control group,the proliferation rates at 24 and 48 h in 50,100, and 200 mg·L-1 deoxyschizandrin groups were significantly decreased(P < 0.01),and the proliferation rates at 72 h in 100 and 200 mg·L-1 deoxyschizandrin groups were significantly decreased(P < 0.05 or P < 0.01).Compared with control group,the percentage of cells at SubG1 phase in 200 mg·L-1 deoxyschizandrin group was increased (P < 0.05),and the percentage of cells at S phase was decreased (P < 0.05). Compared with control group,the expression levels of CyclinD1 in 100 and 200 mg·L-1deoxyschizandrin groups were decreased (P < 0.01 );the expression levels of Bax protein in deoxyschizandrin groups were increased(P < 0.05 or P < 0.01),and the expression level of Bcl-2 protein in 200 mg·L-1 deoxyschizandrin group was decreased(P < 0.01),and the Bax/Bcl-2 value in deoxyschizandrin groups were increased(P < 0.01 );the expression level of Caspase-3 protein in 200 mg·L-1 deoxyschizandrin group was increased(P < 0.01). Conclusion: Deoxyschizandrin could inhibit the growth of glioma cells through down-regulating the expression levels of CyclinD1 protein and up-regulating the expression levels apoptotic factors Bax and Bcl-2.

Key words: deoxyschizandrin, glioma cells, CyclinD1, Bax/Bcl-2

中图分类号: 

  • R739.41