头孢哌酮钠舒巴坦钠对反复下呼吸道感染患者中性粒细胞胞外网状陷阱形成的影响

doi:10.13481/j.1671-587x.20170327

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (03): 607-610.doi: 10.13481/j.1671-587x.20170327

头孢哌酮钠舒巴坦钠对反复下呼吸道感染患者中性粒细胞胞外网状陷阱形成的影响

唐颖, 黄元平, 荆文花, 关英慧

吉林大学第一医院呼吸内科, 吉林长春 130021

收稿日期:2016-08-26 出版日期:2017-05-28 发布日期:2017-06-01
通讯作者: 关英慧,副主任医师,硕士研究生导师(Tel:0431-84808365,E-mail:1186887791@qq.com) E-mail:1186887791@qq.com
作者简介:唐颖(1982-),女,辽宁省沈阳市人,主治医师,医学博士,主要从事呼吸系统疾病免疫机制方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20110489）；吉林大学第一医院二部科研专项基金资助课题（B-24）

Effect of sulbactam sodium/cefoperazone sodium on neutrophil extracellular traps of patients with recurrent lower respiratory tract infection

TANG Ying, HUANG Yuanping, JING Wenhua, GUAN Yinghui

Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, China

Received:2016-08-26 Online:2017-05-28 Published:2017-06-01

摘要/Abstract

摘要： 目的：检测反复下呼吸道感染患者外周血中性粒细胞胞外网状陷阱（NETs）的形成，评价头孢哌酮钠舒巴坦钠对NETs形成的影响。方法：选取反复下呼吸道感染患者36例和健康体检者30名作为病例组和健康对照组。采用激光共聚焦显微镜及扫描电镜检测2组研究对象NETs的形成情况，并根据中性粒细胞的形态将NETs的形成情况分成Ⅰ、Ⅱ和Ⅲ级，计数2组研究对象不同级别NETs形成细胞数量；观察头孢哌酮钠舒巴坦钠处理前后病例组患者NETs的形成情况。结果：病例组患者Ⅰ和Ⅱ级NETs形成细胞数量明显多于健康对照组（P<0.05），而Ⅲ级NETs形成细胞数量少于健康对照组（P<0.05）；头孢哌酮钠舒巴坦钠处理后病例组患者Ⅰ和Ⅱ级NETs形成细胞数量明显少于用药前（P<0.05），而Ⅲ级NETs形成细胞数量多于用药前（P<0.05）。结论：反复下呼吸道感染患者体内中性粒细胞能形成大量具有高效抗菌功能的NETs，而头孢哌酮钠舒巴坦钠可以抑制NETs的形成。

关键词: 头孢哌酮钠舒巴坦钠, 扫描电镜, 激光共聚焦显微镜, 反复下呼吸道感染, 中性粒细胞胞外网状陷阱

Abstract: Objective: To detect the neutrophil extracellular traps (NETs) formation in the peripheral blood of the patients with recurrent respiratory tract infection, and to evaluate the effect of sulbactam sodium/cefoperazone sodium on the formation of NETs. Methods: A total of 36 patients with recurrent respiratory tract infection (case group) and 30 healthy volunteers (healthy control group) were selected. The NETs formation of subjects in two groups was detected by confocal microscope and scanning electron microscope (SEM). According to the appearance of neutrophils, the formation of NETs was classified as grade Ⅰ, Ⅱ and Ⅲ,the number of NETs formation cells of subjects in two groups was calculated. The formation of NETs of the patients in case group were detected before and after treated with sulbactam sodium/cefoperazone sodium. Results: The number of NETs formation cells of grade Ⅰ and Ⅱ of the patients in case group was more than that in healthy control group (P<0.05); while the number of NETs formation cells of grade Ⅲ of the patients in case group was less than that in healthy control group (P<0.05). The number of NETs formation cells of grade Ⅰ and Ⅱ of the patients in case group were significantly decreased (P<0.05), while the number of NETs formation cells of grade Ⅲ was significantly increased (P<0.05) after treated with sulbactam sodium/cefoperazone sodium. Conclusion: A lot of NETs with high antibacterial function can be formed in the patients with recurrent lower respiratory tract infection, and sulbactam sodium/cefoperazone sodium can inhibit the formation of NETs.

Key words: recurrent lower respiratory tract infection, confocal microscope, scanning electron microscope, neutrophil extracellular traps, sulbactam sodium/cefoperazone sodium

中图分类号:

R563

唐颖, 黄元平, 荆文花, 关英慧. 头孢哌酮钠舒巴坦钠对反复下呼吸道感染患者中性粒细胞胞外网状陷阱形成的影响[J]. 吉林大学学报(医学版), 2017, 43(03): 607-610.

TANG Ying, HUANG Yuanping, JING Wenhua, GUAN Yinghui. Effect of sulbactam sodium/cefoperazone sodium on neutrophil extracellular traps of patients with recurrent lower respiratory tract infection[J]. Journal of Jilin University Medicine Edition, 2017, 43(03): 607-610.

参考文献

[1] Brinkmann V, Reichard U, Goosmann C, et al.Neutrophil extracellular traps kill bacteria[J].Science, 2004,303(5663):1532-1535.
[2] Grabcanovic-Musija F, Obermayer A, Stoiber W, et al. Neutrophil extracellular trap (NET) formation characterises stable and exacerbated COPD and correlates with airflow limitation[J]. Respir Res, 2015, 16(1):59.
[3] 杨俊. 研究舒普深在治疗中重度下呼吸道感染中的有效性[J]. 世界最新医学信息文摘, 2015, 15(60):95.
[4] Brinkmann V, Laube B, Abu UA, et al. Neutrophil extracellular traps:how to generate and visualize them[J]. J Vis Exp, 2010, 36(36):77-82.
[5] Obermayer A, Stoiber W, Krautgartner WD, et al. New aspects on the structure of neutrophil extracellular traps from chronic obstructive pulmonary disease and in vitro generation[J]. PLoS One, 2014, 9(5):e97784.
[6] Hamaguchi S, Hirose T, Matsumoto N, et al. Neutrophil extracellular traps in bronchial aspirates:a quantitative analysis[J]. Eur Respir J, 2014, 43(6):1709-1718.
[7] Hamaguchi S, Hirose T, Akeda Y, et al. Identification of neutrophil extracellular traps in the blood of patients with systemic inflammatory response syndrome[J]. J Int Med Res, 2013, 41(1):162-168.
[8] Parker H, Dragunow M, Hampton MB, et al. Requirements for NADPH oxidase and myeloperoxidase in neutrophil extracellular trap formation differ depending on the stimulus[J]. J Leukoc Biol, 2012, 92(4):841-849.
[9] Funchal GA, Jaeger N,Czepielewski RS, et al. Respiratory syncytial virus fusion protein promotes TLR-4-dependent neutrophil extracellular trap formation by human neutrophils[J]. PLoS One, 2015, 10(4):e0124082.
[10] 原铭贞, 高广媛, 李波,等. 过氧化物酶6与肺部炎症性疾病关系的研究进展[J]. 吉林大学学报:医学版, 2014, 40(1):210-214.
[11] Chalmers JD, Hill AT. Mechanisms of immune dysfunction and bacterial persistence in non-cystic fibrosis bronchiectasis[J]. Mol Immunol, 2013, 55(1):27-34.
[12] 金梅. 中性粒细胞外陷阱(NETs)杀灭金黄色葡萄球菌及糖皮质激素对其影响的体外研究[D]. 杭州:浙江大学, 2014.
[13] Gonzalez DJ, Corriden R, Akong-Moore K, et al. N-terminal ArgD peptides from the classical Staphylococcus aureus Agr system have cytotoxic and proinflammatory activities[J]. Chem Biol, 2014, 21(11):1457-1462.
[14] Jenne CN, Wong CH, Zemp FJ, et al. Neutrophils recruited to sites of infection protect from virus challenge by releasing neutrophil extracellular traps[J]. Cell Host Microbe,2013, 13(2):169-180.
[15] Wright TK, Gibson PG, Simpson JL, et al. Neutrophil extracellular traps are associated with inflammation in chronic airway disease[J]. Respirology, 2016, 21(3):467-475.

头孢哌酮钠舒巴坦钠对反复下呼吸道感染患者中性粒细胞胞外网状陷阱形成的影响

Effect of sulbactam sodium/cefoperazone sodium on neutrophil extracellular traps of patients with recurrent lower respiratory tract infection

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

Metrics

本文评价

推荐阅读 0

[1]	牟胤赫, 杨陆一, 吴嫣然, 滕蓉, 夏小雪, 宁磊, 王守东. 不同条件下3种正畸粘接剂粘结强度的比较及其意义[J]. 吉林大学学报(医学版), 2016, 42(03): 512-516.
[2]	姜俊如，刘岚，沈犁，楚丽娟，张佳星，王磊，周薇，伏小红. 不可分型流感嗜血杆菌生物膜体外形成规律及其扫描电镜形态学[J]. 吉林大学学报(医学版), 2014, 40(04): 729-733.
[3]	李家奇，孟维艳，周延民，蔡青，阿兰，付丽，李保胜，王世振. 不同表面形貌的纯钛种植体对牙龈软组织界面影响的扫描电镜观察[J]. 吉林大学学报(医学版), 2014, 40(01): 102-108.