吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 782-786.doi: 10.13481/j.1671-587x.20170423

• 临床研究 • 上一篇    下一篇

IL-10和IL-12检测在结核性和恶性胸腔积液鉴别诊断中的应用

刘红梅1, 初喆2, 田锐3, 唐颖1   

  1. 1. 吉林大学第一医院呼吸内科, 吉林 长春 130021;
    2. 吉林大学第一医院急诊内科, 吉林 长春 130021;
    3. 空军航空大学门诊部, 吉林 长春 130022
  • 收稿日期:2016-08-17 出版日期:2017-07-28 发布日期:2017-08-01
  • 通讯作者: 唐颖,主治医师(Tel:0431-88783442,E-mail:tang_ying@jlu.edu.cn) E-mail:tang_ying@jlu.edu.cn
  • 作者简介:刘红梅(1969-),女,吉林省长春市人,主管护师,主要从事呼吸内镜在呼吸系统疾病诊断和治疗中应用的研究。
  • 基金资助:
    吉林省科技厅科技发展计划项目资助课题(20110489);吉林省科技厅医药产业发展专项资金项目资助课题(20130727040YY);吉林大学白求恩培育项目资助课题(2015328)

Application of IL-10 and IL-12 detection in differential diagnosis of tuberculous and malignant pleural effusion

LIU Hongmei1, CHU Zhe2, TIAN Rui3, TANG Ying1   

  1. 1. Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, China;
    2. Department of Emergency, First Hospital, Jilin University, Changchun 130021, China;
    3. Department of Out-patients, Aviation University of Air Force, Changchun 130022, China
  • Received:2016-08-17 Online:2017-07-28 Published:2017-08-01

摘要: 目的:通过检测结核性和恶性胸腔积液患者血清和胸腔积液中白细胞介素10(IL-10)和白细胞介素12(IL-12)的水平,探讨二者对于鉴别结核性和恶性胸腔积液的价值。方法:选取住院未经治疗的渗出性胸腔积液患者48例,根据病因分为结核性胸腔积液组25例和恶性胸腔积液组23例。采用流式微球阵列法(CBA)检测2组患者血清和胸腔积液中IL-10和IL-12的水平,比较2组患者IL-10、IL-12水平和IL-12/IL-10比值的差异;通过受试者工作特征(ROC)曲线分析上述指标与胸水脱落细胞和腺苷脱氢酶(ADA)在鉴别结核性和恶性胸腔积液中的作用。结果:结核性胸腔积液组和恶性胸腔积液组患者血清中IL-10、IL-12水平和IL-12/IL-10比值比较差异无统计学意义(P> 0.05),恶性胸腔积液组患者胸腔积液中IL-12水平和IL-12/IL-10比值明显低于结核性胸腔积液组(P< 0.01)。利用胸腔积液IL-12水平鉴别结核性和恶性胸腔积液的ROC曲线下面积为0.984,明显高于IL-12/IL-10比值(0.744)、脱落细胞(0.804)和ADA(0.911)。结论:胸腔积液中IL-12水平检测有助于结核性和恶性胸腔积液的鉴别诊断,且标本容易获取,值得临床上推广应用。

关键词: 结核性胸腔积液, 白细胞介素10, 恶性胸腔积液, 白细胞介素12

Abstract: Objective: To investigate the role of interleukin-10 (IL-10) and IL-12 in the differential diagnosis of tuberculous and malignant pleural effusion through measuring the serum and pleural effusion IL-10 and IL-12 levels of tuberculous and malignant pleural effusion patients.Methods: Forty-eight inpatients with exudative pleural effusion who didn't receive any treatment before were selected. According to the pathogeny, the patients were divided into tuberculous pleural effusion group (n=25) and malignant pleural effusion group (n=23). The levels of IL-10 and IL-12 in peripheral blood and pleural effusion of the patients in two groups were detected by Cytometric Bead Array (CBA). The differences in IL-10, IL-12 levels and IL-12/IL-10 ratio were compared between two groups. The ROC curves of the above indexes, cast-off and adenosine deaminase (ADA) were used to compare the effects in differential diagnosis of tuberculous and malignant pleural effusion.Results: There were no statistical differences in the levels of serum IL-10, IL-12 and IL-12/IL-10 ratios of the patients between tuberculous and malignant pleural effusion groups (P>0.05),and the IL-12 level in pleural effusion and the IL-12/IL-10 ratio of the patients in malignant pleural effusion group were lower than those in tuberculous pleural effusion group (P<0.005).The area under the ROC curve for the levels of IL-12 in pleural effusion (0.984) was higher than those for the ratio of IL-12/IL-10, cast-off and ADA (0.744, 0.804 and 0.911, respectively) in the differential diagnosis of tuberculous and malignant pleural effusion.Conclusion: The detection of IL-12 levels in pleural effusion is helpful for the differential diagnosis of tuberculous and malignant pleural effusion, and the pleural effusion is easy to obtain, therefore the detection of IL-12 in pleural effusion is worth to be widely applied in clinic.

Key words: interleukin-10, interleukin-12, tuberculous pleural effusion, malignant pleural effusion

中图分类号: 

  • R561.1