关键词: 乙二醛酶I, 泛素羧基末端水解酶L1, 热休克蛋白27, 血清淀粉样蛋白P, 卵泡发育

Abstract: Objective:To detect the expressions of ovarian function-related proteins in the ovarian follicles in different development stages, and to explore their roles and significances in the regulation of follicular development and ovarian function. Methods:The ovarian tissue containing primordial, primary, secondary follicles, mature follicles,atretic follicles and corpus albicans of the woman in the childbearing age was selected.Immunohisto chemical analysis was used to detect the expressions of Glyoxal enzyme I (glyoxalase I), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), heat shock protein 27 (HSP27), serum amyloid P (SAP) proteins in the ovarian follicles in different stages.The expression intensities of these proteins were compared. Results:The expression intensities of Glyoxalase I and UCH-L1 in the granulosa cells and theca cells of secondary follicles and marure follicles were strong, which were higher than those in the cytoplasm of primordial and primary follicles. The expression intensities of HSP27 in the cytoplasm of primordial and primary follicles were strong, which were higher than those in the granulosa cells and theca cells of secondary follicles and marure follicles.The expression intensities of Glyoxalase I and HSP27 in the atretic follicles were strong,which were higher than that in the growth follicles.The expressions of SAP were positive in the primordial, primary follicles, secondary and mature follicles and the expression intensities were not different. The expressions of four proteins in the corpus albicans did not express. Conclusion:The high expression of UCH-L1 and low expression of HSP27 are associated with the mature and development of follicles; the high expressions of Glyoxalase I and HSP27 are associated with the follicular atresia and ovarian failure.

Key words: serum amyloid p, follicle development, glyoxal enzyme I, ubiquitin carboxy-terminal hydrolase L1, heat shock protein 27