白藜芦醇对肝癌HepG2细胞中脂肪合成的抑制作用及其机制

doi:10.13481/j.1671-587x.20190113

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (01): 69-72.doi: 10.13481/j.1671-587x.20190113

• 基础研究 • 上一篇

白藜芦醇对肝癌HepG₂细胞中脂肪合成的抑制作用及其机制

苗向霞¹, 郭蕊², 张璎¹, 卫银银¹, 罗正奇¹, 闵亚丽¹, 刘凯歌³

1. 西安医学院研究生院, 陕西西安 710068;
2. 宁夏医科大学研究生院, 宁夏银川 750004;
3. 西安医学院第一附属医院消化内科, 陕西西安 710077

收稿日期:2018-07-12 发布日期:2019-01-28
通讯作者: 刘凯歌,教授,硕士研究生导师(Tel:029-84270539,E-mail:kaigeliu007@163.com) E-mail:kaigeliu007@163.com
作者简介:苗向霞(1992-),女,陕西省榆林市人,在读医学硕士,主要从事肝脏脂代谢方面的研究。

Inhibitory effect of resveratrol on fat synthesis in liver cancer HepG₂ cells and its mechanism

MIAO Xiangxia¹, GUO Rui², ZHANG Ying¹, WEI Yinyin¹, LUO Zhengqi¹, MIN Yali¹, LIU Kaige³

1. Graduate School, Xi'an Medical University, Xi'an 710068, China;
2. Graduate School, Ningxia Medical University, Yinchuan 750004, China;
3. Department of Digestive Medicine, First Affiliated Hospital, Xi'an Medical College, Xi'an 710077, China

Received:2018-07-12 Published:2019-01-28
Contact: 陕西省教育厅科研项目资助课题（11JK0708）；陕西省科技厅科研项目资助课题（2016SF-327） E-mail:kaigeliu007@163.com

摘要/Abstract

摘要： 目的：探讨白藜芦醇（Res）对肝癌HepG₂细胞脂肪合成的影响，阐明其可能机制。方法：体外培养HepG₂细胞，分为Res组（40μmol·L^-1DMSO稀释的Res作用24h）和对照组（相同浓度的DMSO作用24h）。收集细胞上清，ELISA法检测2组细胞中甘油三酯（TG）和总胆固醇（TC）水平。采用实时荧光定量PCR （qRT-PCR）和Western blotting法分别检测2组细胞中脂肪合成酶乙酰辅酶A羧化酶（ACC1）、脂肪酸合成酶（FASN）、硬脂酰辅酶A去饱和酶（SCD1） mRNA和蛋白表达水平，采用Western blotting法检测2组细胞中氧链接N乙酰葡糖胺（O-GlcNAc）糖基化水平。结果：与对照组比较，Res组细胞中TG和TC水平均下降，但差异无统计学意义（t₁=1.886，P<0.05；t₂=2.457，P>0.05）；与对照组比较，Res组细胞中ACC1、FASN、SCD1 mRNA和蛋白表达水平均明显降低（P<0.05或P<0.01），O-GlcNAc糖基化水平明显降低（t=2.87，P<0.05）。结论：Res具有抑制肝癌HepG₂细胞脂肪合成的作用，其机制可能与降低细胞中O-GlcNAc糖基化水平和FASN表达有关。

关键词: 白藜芦醇, 非酒精性脂肪肝, 肝癌HepG₂细胞, 脂肪酸合成酶, 氧链接N乙酰葡糖胺糖基化

Abstract: Objective: To investigate the effect of resveratrol (Res) on the fat synthesis in the liver cancer HepG₂ cells, and to elucidate its possible mechanism.Methods: The HepG₂ cells were cultured in vitro and divided into Res group (treated with 40 μmol·L^-1 DMSO- diluted Res for 24 h) and control group (treated with the same concentration of DMSO for 24 h). The cell supernatant was collected, and the levels of triglyceride (TG) and total cholesterol (TC) in the cells in various groups were measured by ELISA. The mRNA and protein expression levels of lipase synthase acetyl-CoA carboxylase (ACC1), fatty acid synthetase (FASN) and stearoyl-CoA desaturase (SCD1) in the cells in various groups were detected by qRT-PCR and Western blotting method. The levels of O-linked N-acetylglucosamine(O-GlcNAc)glycosylation in the cells in various groups were detected by Western blotting method.Results: Compared with control group, the levels of TG and TC in the cells in Res group were decreased, but the difference was not statistically significant (t₁=1.886,P>0.05; t₂=2.457,P>0.05). Compared with control group, the levels of expressions of ACC1, FASN and SCD1 mRNA and proteins in the cells in Res group were significantly decreased (P<0.05 or P<0.01); the O-GlcNAc glycosylation level in the cells in Res group was significantly decreased (t=2.87, P<0.05).Conclusion: Res has the effect of inhibiting the fat synthesis in the liver cancer HepG₂ cells. Its mechanism may be related to the reduction of cellular O-GlcNAc glycosylation level and the reduction of the expression of FASN.

Key words: resveratrol, non-alcoholic fatty liver disease, HepG₂ cells, fatty acid synthetase, O-GlcNAc glycosylation

中图分类号:

R285.5

苗向霞, 郭蕊, 张璎, 卫银银, 罗正奇, 闵亚丽, 刘凯歌. 白藜芦醇对肝癌HepG₂细胞中脂肪合成的抑制作用及其机制[J]. 吉林大学学报(医学版), 2019, 45(01): 69-72.

MIAO Xiangxia, GUO Rui, ZHANG Ying, WEI Yinyin, LUO Zhengqi, MIN Yali, LIU Kaige. Inhibitory effect of resveratrol on fat synthesis in liver cancer HepG₂ cells and its mechanism[J]. Journal of Jilin University(Medicine Edition), 2019, 45(01): 69-72.

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白藜芦醇对肝癌HepG₂细胞中脂肪合成的抑制作用及其机制

Inhibitory effect of resveratrol on fat synthesis in liver cancer HepG₂ cells and its mechanism

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