姜黄素对骨质疏松大鼠种植体骨结合的促进作用

doi:10.13481/j.1671-587x.20190423

摘要/Abstract

摘要： 目的：探讨姜黄素对骨质疏松大鼠种植体骨结合的影响，阐明其的作用机制。方法：45只大鼠随机分为对照组（植入螺纹钉）、模型组（骨质疏松模型+植入螺纹钉）和姜黄素组（骨质疏松模型+植入螺纹钉+姜黄素治疗），每组15只。显微CT检查各组大鼠种植体周围骨组织的相对骨体积分数（BV/TV）、平均骨小梁粗度（Tb.Th）、骨小梁间隙（Tb.Sp）、平均骨小梁数量（Tb.N）和骨结合指数（OI），亚甲基蓝-碱性品红染色检查骨组织形态表现，计算骨结合率和松质骨区骨量，扭矩测试仪测定种植体脱位扭矩。结果：与对照组比较，模型组和姜黄素组大鼠种植体周围骨界面BV/TV、Tb.Th、Tb.N、OI、骨结合率、松质骨区骨量和脱位扭矩均明显降低（P<0.01），Tb.Sp明显升高（P<0.01）；与模型组比较，姜黄素组大鼠种植体周围骨界面BV/TV、Tb.Th、Tb.N、OI、骨结合率、松质骨区骨量和脱位扭矩明显升高（P<0.01），Tb.Sp明显降低（P<0.01）。对照组大鼠种植体和骨结合紧密，种植体骨结合界面骨板较厚，骨小梁较密集；模型组大鼠种植体骨结合界面骨板比较薄，骨小梁稀疏；姜黄素组大鼠种植体骨结合界面骨板较厚，骨小梁较密集。结论：姜黄素可促进骨质疏松大鼠种植体愈合，改善种植体骨结合强度，增加种植体稳定性。

关键词: 姜黄素, 骨质疏松, 种植体, 骨结合, 大鼠

Abstract: Objective:To investigate the effect of curcumin on the implant osseointegration in the rats with osteoporosis, and to clarify its mechanism. Methods:A total of 45 rats were randomly divided into control group (screw implant), model group (osteoporosis model + screw implant) and curcumin group (osteoporosis model + screw implant + curcumin treatment), with 15 rats in each group. The micro-CT was used to examine the relative bone volume fraction (BV/TV), average bone trabecular thickness (Tb. Th), trabecular space (Tb.Sp), average bone trabecular number (Tb.N),osseointegration index(OI) of the bone tissue around the implants of the rats in various groups; methylene blue-basic fuchsin staining was used to examine the morphology of bone tissue;the bone contact rate and the bone mass in cancellous bone area were calculated. The torque tester was used to measure the implant dislocation torque. Results:Compared with control group, the BV/TV, Tb.Th, Tb.N, OI,the bone contact rates,the bone mass in cancellous bone area,and the dislocation torque of bone interface around the implants of the rats in model group and curcumin group were significantly decreased (P<0.01), while Tb.Sp in model group and curcumin group were significantly increased (P<0.01). Compared with model group, the BV/TV, Tb.Th, Tb.N, OI, the bone contact rate, the bone mass in cancellous bone area,and the dislocation torque of bone interface around the implants of the rats in curcumin group were increased (P<0.01),while Tb.Sp was significantly decreased (P<0.01). In control group, the bonding of implant and bone was close, and the bone plate and trabeculae at the bonding interface of implant and bone were thicker and denser. In model group, the bone plate at the bonding interface of implant and bone was thin and the trabecular bone was sparse. In curcumin group, the bone plate and trabeculae at bonding interface of the implant and bone of the rats were thicker and denser. Conclusion:Curcumin can promote the implant healing, improve the implant and bone bonding strength and increase the implant stability in the osteoporosis rats.

Key words: curcumin, osteoporosis, implant, osseointegration, rats

中图分类号:

R285.5

许东亮, 彭朝晖, 熊美才. 姜黄素对骨质疏松大鼠种植体骨结合的促进作用[J]. 吉林大学学报(医学版), 2019, 45(04): 877-881.

XU Dongliang, PENG Zhaohui, XIONG Meicai. Promotion effect of curcumin on implant osseointegration in osteoporosis rats[J]. Journal of Jilin University(Medicine Edition), 2019, 45(04): 877-881.

参考文献

[1] CAI J, LI W, SUN T,et al.Pulsed electromagnetic fields preserve bone architecture and mechanical properties and stimulate porousimplantosseointegrationby promoting bone anabolism in type 1 diabetic rabbits[J].Osteoporos Int,2018,29(5):1177-1191.
[2] DUAN Y, MA W, LI D,et al.Enhancedosseointegration of titanium implants in a rat model of osteoporosis using multilayer bone mesenchymal stem cell sheets[J].Exp Ther Med,2017,14(6):5717-5726.
[3] ZHAO R, XIE PF, ZHANG K, et al. Selective effect of hydroxyapatite nanoparticles on osteoporotic and healthy bone formation correlates with intracellular calcium homeostasis regulation[J]. Acta Biomater, 2017, 59:338-350.
[4] CHOI Y H, HAN Y, JIN S W, et al. Pseudoshikonin Ⅰ enhances osteoblast differentiation by stimulating Runx2 and Osterix[J]. J Cell Biochem, 2018, 119(1):748-757.
[5] PEDDADA K V, PEDDADA K V, SHUKLA S K,et al.Role of curcumin in common musculoskeletal disorders:a review of current laboratory, translational, and clinical data[J].Orthop Surg,2015,7(3):222-231.
[6] XIN M, YANG Y, ZHANG D,et al.Attenuation of hind-limb suspension-induced bone loss by curcumin is associated with reduced oxidative stress and increased vitamine D receptor expression[J].Osteoporos Int,2015,26(11):2665-2676.
[7] APOSTU D, LUCACIU O, BERCE C,et al.Current methods of preventing aseptic loosening and improving osseointegration of titanium implants in cementless total hip arthroplasty:a review[J].J Int Med Res,2018,46(6):2104-2119.
[8] OKI Y, DOI K, MAKIHARA Y,et al.Effects of continual intermittent administration of parathyroid hormone on implant stability in the presence of osteoporosis:An in vivo study using resonance frequency analysis in a rabbit model[J].J Appl Oral Sci,2017,25(5):498-505.
[9] GHANEM A, KELLESARIAN S V, ABDULJABBAR T,et al.Role of osteogenic coatings on implant surfaces in promoting bone-to-implant contact in experimental osteoporosis:A systematic review and meta-analysis[J]. Implant Dent, 2017,26(5):770-777.
[10] LIU YM, HU J, LIU B,et al.The effect of osteoprotegerin on implant osseointegration in ovariectomized rats[J].Arch Med Sci,2017,13(2):489-495.
[11] ZHOU HB, HOU YF, ZHU ZM,et al.Effects of low-intensity pulsed ultrasound on implant osseointegration in ovariectomized rats[J].J Ultrasound Med,2016,35(4):747-754.
[12] HUANG YM, LIN Y, WU YS, et al. Molecular mechanisms of the inhibitory effects of jiangu granule-containing serum on RANKL-induced osteoclastogenesis[J]. Mol Med Rep, 2017, 16(6):8420-8426.
[13] 王买全,李运峰.同时应用雌激素和二膦酸盐对骨质疏松大鼠种植体骨结合的累加效应[J].实用口腔医学杂志,2016,32(5):640-644.
[14] JEBAHI S, SAOUDI M, FARHAT L,et al.Effect of novel curcumin-encapsulated chitosan-bioglass drug on bone and skin repair after gamma radiation:experimental study on a Wistar rat model[J].Cell Biochem Funct,2015,33(3):150-159.
[15] RIVA A, TOGNI S, GIACOMELLI L,et al.Effects of a curcumin-based supplementation in asymptomatic subjects with low bone density:A preliminary 24-week supplement study[J].Eur Rev Med Pharmacol Sci,2017,21(7):1684-1689.
[16] 李刚,刘莹,种铁,等.姜黄素对裸鼠人ACHN肾癌移植瘤的放射增敏作用[J].西安交通大学学报:医学版,2019,40(2):284-289.
[17] 陈之光,薛今琦,付勤,等.姜黄素对骨骼系统的影响[J].中华骨质疏松和骨矿盐疾病杂志,2016,9(3):323-329.
[18] CHEN Z, XUE J, SHEN T,et al.Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway[J].Int J Mol Med,2016,37(2):329-338.
[19] CHO D C, RYU K, KIM K T,et al.The therapeutic effects of combination therapy with curcumin and alendronate on spine fusion surgery in the ovariectomized rats[J].Korean J Spine,2017,14(2):35-40.
[20] 李义,张建中,宋英,等.姜黄素对去卵巢骨质疏松模型鼠的机制研究[J].中成药,2013,35(7):1359-1362.
[21] 马如风,王丽丽,左加成,等.姜黄素通过调节组织蛋白酶K改善高脂诱导C57BL/6J小鼠骨结构和骨质量的实验研究[J].中国药理学通报,2017,33(10):1446-1451.