关键词: 非人工呼吸机, 心肌缺血再灌注损伤, 无复流现象, 疾病模型,动物

Abstract: Objective:To establish the rat models of myocardial ischemia reperfusion-related no-reflow under non-artificial ventilator, to evaluate the models by morphology, hematological biochemistry and hemorheology, and to lay the foundation for studying the pathogenesis of cardiovascular diseases and evaluating the pharmacodynamics of drugs of cardiovascular diseases. Methods:Fifty healthy female Wistar rats were randomly divided into sham operation group (n=20) and model group (n=30). The myocardial ischemia reperfusion-related no-reflow rat models in model group were induced by ligation of left anterior descending coronary artery for 2 h and another 2 h for reperfusion. The rats in sham group were only threaded and not ligated. The model was evaluated by detecting the area at risk (AAR), area at infarct (AAI), area at no-reflow (AAN) of myocardium, the activities of creatine kinase MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) in serum,the whole blood viscosity,the plasma viscosity, the platelet adhesion rate (PAR),the platelet aggregation (PAG) of the rats. Results:Compared with sham operation group, the AAR, AAI,and AAN of myocardium, the activities of CK-MB, LDH,and AST in serum,the whole blood low shear viscosity(20/s),the middle shear viscosity(60/s), the high shear viscosity(120/s),the plasma viscosity, PAR, PAG (1, 3, 5 min)and the maximum platelet aggregation rate(MAPG) of the rats in model group were significantly increased (P<0.05 or P<0.01). Conclusion:The rat model of myocardial ischemia reperfusion-related no-reflow under non-artificial ventilator is successfully established. This method is characterized by simple operation, decreased injury to animals, higher stability of model construction, shorter experimental cycle and less cost.

Key words: non-artificial ventilator, myocardial ischemia reperfusion injury, no-reflow phenomenon, disease model, animal