CpG ODN BW006对实验性牙移动模型大鼠牙周组织中Runx2和Osterix表达的影响及其意义

doi:10.13481/j.1671-587x.20200222

吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (02): 340-345.doi: 10.13481/j.1671-587x.20200222

• 基础研究 • 上一篇

CpG ODN BW006对实验性牙移动模型大鼠牙周组织中Runx2和Osterix表达的影响及其意义

黄蕾^1,2, 刘宇妍¹, 于文雯¹, 周雪纯¹, 张骁³, 申玉芹⁴, 郑义⁴, 孙新华¹

1. 吉林大学口腔医院正畸科, 吉林长春 130021;
2. 吉林省牙发育及颌骨重塑与再生重点实验室, 吉林长春 130021;
3. 吉林大学口腔医院修复科, 吉林长春 130021;
4. 吉林大学口腔医院牙周病科, 吉林长春 130021

收稿日期:2019-12-09 发布日期:2020-04-07
通讯作者: 孙新华,教授,博士研究生导师(Tel:0431-88796023,E-mail:xinhuasun8@163.com) E-mail:xinhuasun8@163.com
作者简介:黄蕾(1993-),女,内蒙古自治区包头市人,在读医学硕士,主要从事正畸及牙周组织改建方面的研究。
基金资助:
吉林省科技厅医药健康产业发展专项资金资助课题（20170311032YY）；吉林省财政厅科研基金资助课题（jsz2018170-12）

Effect of CpG ODN BW006 on expressions of Runx2 and Osterix in periodontal tissue of experimental tooth movement model rats and its significance

HUANG Lei^1,2, LIU Yuyan¹, YU Wenwen¹, ZHOU Xuechun¹, ZHANG Xiao³, SHEN Yuqin⁴, ZHENG Yi⁴, SUN Xinhua¹

1. Department of Orthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
2. Jilin Provincial Key Laboratory of Tooth Development and Jaw Remodeling and Regeration, Changchun 130021, China;
3. Department of Prosthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
4. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2019-12-09 Published:2020-04-07

摘要/Abstract

摘要： 目的：通过观察免疫刺激性脱氧寡核苷酸（CpG ODN）BW006对实验性牙移动大鼠牙周组织中成骨相关转录因子Runx2和Osterix表达的影响，探讨其对正畸牙移动大鼠牙周组织改建的作用。方法：选取36只7周龄雄性Wistar大鼠建立实验性牙移动模型，左侧上颌为实验组（局部注射CpG ODN BW006），右侧上颌为对照组（局部注射PBS），每3 d给药1次，每次40 μL，于加力3、7和14 d时分别随机选取12只大鼠处死，取含有第一磨牙的上颌骨组织制备标本，进行HE染色和免疫组织化学染色，观察牙周组织形态表现，测量牙齿移动距离，检测牙周组织中Runx2和Osterix的表达。结果：HE染色，实验组大鼠压力侧骨吸收程度较低，对照组大鼠压力侧骨吸收明显，实验组大鼠张力侧可见大量成骨细胞，牙槽骨丰满度及高度高于对照组；加力7和14 d时实验组牙齿移动距离分别为（0.347±0.007）和（0.443±0.016）mm，对照组分别为（0.585±0.012）和（0.975±0.084）mm，实验组与对照组牙齿移动距离比较差异有统计学意义（P<0.01）。免疫组织化学染色，加力3和7 d时实验组大鼠牙周组织中Runx2和Osterix阳性表达水平明显升高，与对照组比较差异有统计学意义（P<0.05或P<0.01），且均于加力7 d时达到峰值；加力14 d时实验组Runx2和Osterix阳性表达水平略有下降，但与对照组比较差异有统计学意义（P<0.05或P<0.01）。结论：CpG ODN BW006可通过促进牙移动过程中Runx2和Osterix的表达调控牙周组织改建。

关键词: 免疫刺激性脱氧寡核苷酸, Runt2, Osterix, 牙周组织改建

Abstract: Objective: To observe the effect of immunostimulatory oligodeoxynucleotide (CpG ODN) BW006 on the expressions of Runt-related transcription factor 2(Runx2) and Osterix in periodontal tissue of the rats with experimental tooth movement, and to explore its effect on the periodontal remodeling of the rats with orthodontic tooth movement. Methods: The experimental tooth movement models were established in the 36 7-week-old male Wistar rats. The left maxilla was used as experimental group (local injection of CpG ODN BW006) and the right maxilla as control group (local injection of PBS), once every 3 d, 40 μL each time. Twelve rats were randomly selected at 3, 7 and 14 d after stress, and the maxillary tissue containing the first molars was prepared. HE staining and immunohistochemical staining were performed to observe the morphological changes of periodontal tissue, measure the tooth movement distance and detect the expressions of Runx2 and Osterix. Results: The HE staining results showed that the bone resorption on the pressure side of the rats in experimental group was lower than that in control group, and a large number of osteoblasts were seen on the tension side of the rats in experimental group; the alveolar bone fullness and height of the rats in experimental group were significantly higher than those in control group. The distances of tooth movement in experimental group were (0.347±0.007) and (0.443±0.016)mm, while they were (0.585±0.012) and (0.975±0.084)mm in control group at 7 and 14 d; there were significantly differences between two groups(P<0.01). The immunohistochemical staining results showed that the positive expression levels of Runx2 and Osterix in the periodontal tissue of the rats in experimental group were significantly higher than those in control group at 3 and 7 d (P<0.05 or P<0.01), and reached a peak on the 7th day; the positive expression levels of Runx2 and Osterix in experimental group were decreased slightly on the 14th day, but there were still significant differences compared with control group (P<0.05 or P<0.01). Conclusion: CpG ODN BW006 can regulate the periodontal tissue remodeling by promoting the expressions of Runx2 and Osterix during tooth movement.

Key words: immunostimulatory oligodeoxynucleotide, Runt2, Osterix, periodontal tissue remodeling

中图分类号:

R783.5

黄蕾, 刘宇妍, 于文雯, 周雪纯, 张骁, 申玉芹, 郑义, 孙新华. CpG ODN BW006对实验性牙移动模型大鼠牙周组织中Runx2和Osterix表达的影响及其意义[J]. 吉林大学学报(医学版), 2020, 46(02): 340-345.

HUANG Lei, LIU Yuyan, YU Wenwen, ZHOU Xuechun, ZHANG Xiao, SHEN Yuqin, ZHENG Yi, SUN Xinhua. Effect of CpG ODN BW006 on expressions of Runx2 and Osterix in periodontal tissue of experimental tooth movement model rats and its significance[J]. Journal of Jilin University(Medicine Edition), 2020, 46(02): 340-345.

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CpG ODN BW006对实验性牙移动模型大鼠牙周组织中Runx2和Osterix表达的影响及其意义

Effect of CpG ODN BW006 on expressions of Runx2 and Osterix in periodontal tissue of experimental tooth movement model rats and its significance

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