美学区切牙拔牙创大鼠模型的建立和改良微创拔牙手术的效果评价

doi:10.13481/j.1671-587x.20200313

吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (03): 504-508.doi: 10.13481/j.1671-587x.20200313

• 基础研究 • 上一篇

美学区切牙拔牙创大鼠模型的建立和改良微创拔牙手术的效果评价

潘永生^1,2, 邝融^1,2, 孙铎², 毛天娇², 姜可新², 李江²

1. 吉林省牙发育及颌骨重塑与再生重点实验室, 吉林长春 130021;
2. 吉林大学口腔医院口腔修复科, 吉林长春 130021

收稿日期:2019-10-20 发布日期:2020-06-11
通讯作者: 李江,教授,主任医师,博士研究生导师(Tel:0431-88796018,E-mail:ljiang@jlu.edu.cn) E-mail:ljiang@jlu.edu.cn
作者简介:潘永生(1991-),男,安徽省宿州市人,住院医师,医学硕士,主要从事口腔修复学方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20170204009YY）；吉林省长春市科技局科技计划项目资助课题（17YJ001）；吉林省发改委和财政厅省级产业创新专项资金项目资助课题（2016C048-3）

Establishment of incisor extraction wound ratmodels in aesthetic area and evaluation on effectiveness of modified minimally invasive tooth extraction surgery

PAN Yongsheng^1,2, KUANG Rong^1,2, SUN Duo², MAO Tianjiao², JIANG Kexin², LI Jiang²

1. Jilin Provincial Key Laboratory of Tooth Development and Jaw Bone Remodeling and Regeneration, Changchun 130021, China;
2. Department of Prosthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2019-10-20 Published:2020-06-11

摘要/Abstract

摘要： 目的：探讨美学区切牙拔牙创大鼠模型的建立和改良微创拔牙手术的应用效果，为建立美学区切牙拔牙创模型及提高牙槽骨和软组织愈合效果提供依据。方法：24只SD大鼠随机分为实验组和对照组（n=12），分别在手术前的第9、6和3天沿着牙龈组织上方磨除大鼠右下切牙的牙冠组织，在建模的第10天进行切牙拔除手术。对照组大鼠采用常规切牙拔除手术，实验组大鼠采用改良的微创手术方式拔除切牙。观察切牙拔除手术前后2组大鼠存活率和体质量，检测2组大鼠切牙拔除的完整程度，并计算切牙完整拔除率，比较实验组与对照组大鼠在切牙拔除手术后1周拔牙创周围软组织的愈合情况以及术后4周大鼠下颌切牙牙槽骨愈合情况。结果：实验组大鼠存活率高于对照组（P<0.05）。术后各时间点实验组大鼠体质量高于对照组（P<0.05）。与对照组比较，实验组大鼠切牙完整拔除率升高（P<0.01）。实验组大鼠拔牙创周围软组织在拔牙后1周内完全愈合，4周后在牙槽脊顶具有大量的新生骨；对照组大鼠在拔牙后1周拔牙创周围软组织仍有红肿现象，拔牙后4周牙槽骨的牙槽脊顶处骨小梁结构紊乱，新生骨结构不良。结论：改良微创拔牙手术可以明显改善大鼠切牙的完整拔除效果，术后拔牙创周围软组织和牙槽骨在相应时间内愈合良好。

关键词: 美学区拔牙创, 微创拔牙术, 软组织愈合, 牙槽骨

Abstract: Objective: To explore the establishment of the incisor extraction wound models in aesthetic area of the rats and the application effect of modified minimally invasive tooth extraction surgery, and to provide the basis for establishment of the incisor extraction wound models in aesthetic area and improvement of the healing of alveolar bone and soft tissue. Methods: Twenty-four SD rats were randomly divided into control group and experiment group (n=12). The crowns of right lower incisors of the rats were removed along with the top of gingival tissue on the 9th, 6th and 3rd days before the incisors extraction, and then the incisor were extracted on the 10th day of experimental modeling. In experiment group, the incisors of rats were extracted by the modified minimally invasive incisor extraction, while in control group the conventional extraction was used to extract the incisor of the rats. The survival rates and body weights of the rats in two groups were observed before and after the incisor extraction. The complete degrees of incisor extraction of the rats in two groups were detected,and the rates of complete incisor extraction were calculated; the healing condition of soft tissue around the incisor extraction of the rats in two groups 1 week after incisor extraction and the recovery condition of mandibular alveolar bone of the rats 4 weeks after incisor extraction were compared. Results: The survival rate of the rats in experiment group was higher than that in control group (P<0.05). The body weights of the rats in experiment group at different time points after extraction were higher than those in control group (P<0.05). Compared with control group, the rate of complete incisor extration of the rats in experiment group was increased(P<0.01).The soft tissue around the incisor extraction wound of the rats in experiment group was completely healed within one week after incisor extraction, and new bone was found at the top of alveolar ridge four weeks after incisor extraction; meanwhile, the soft tissue around the incisor extraction wound of the rats in control group was still red and swollen one week after incisor extraction, and the trabecular structure of bone at the top of alveolar ridge was disordered found weeks after incisor extracion, and the new bone structure was poor. Conclusion: The modified minimally invasive tooth extraction surgery can significantly improve the complete extraction effect of incisors in the rats, and the soft tissue around the incisor extraction wound and the alveolar bone are healed well after minimally invasive tooth extraction.

Key words: extraction wound in aesthetic area, minimally invasive extraction, soft tissue healing, alveolar bone

中图分类号:

R782.05

潘永生, 邝融, 孙铎, 毛天娇, 姜可新, 李江. 美学区切牙拔牙创大鼠模型的建立和改良微创拔牙手术的效果评价[J]. 吉林大学学报(医学版), 2020, 46(03): 504-508.

PAN Yongsheng, KUANG Rong, SUN Duo, MAO Tianjiao, JIANG Kexin, LI Jiang. Establishment of incisor extraction wound ratmodels in aesthetic area and evaluation on effectiveness of modified minimally invasive tooth extraction surgery[J]. Journal of Jilin University(Medicine Edition), 2020, 46(03): 504-508.

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美学区切牙拔牙创大鼠模型的建立和改良微创拔牙手术的效果评价

Establishment of incisor extraction wound ratmodels in aesthetic area and evaluation on effectiveness of modified minimally invasive tooth extraction surgery

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