关键词: 核酸, 恒温扩增, 免疫层析, 布鲁菌病

Abstract: Objective  To investigate the application  value  of nucleic acid isothermal amplification-immunochromatographic test in the diagnosis of brucellosis
and to provide experimental basis for clinical application of nucleic acid test.Methods The brucella nucleic acid was extracted from blood and serum samples, and  isothermal amplification technique was applied to amplify nucleic acid and  immunochromatographic test device was used to interpret the results of nucleic acid amplification directly.Tube agglutination test (SAT) was used as immunological tests.The pairing Chi-square test was performed  in the data analyses.Results The brucella nucleic acid was extracted from the  whole blood and serum of 29 cases,the amplification rate of whole blood nucleic acid was 55.2%,the amplification rate of serum nucleic acid was 23.1%,and there was significant difference (P<0.05).The nucleic acid was extracted from the whole blood of 190 cases in different course,using SAT diagnostic technique,the cases were divided into positive and negative treatment
 populations,to these people,the agreement rate of the nucleic acid test results and SAT test results was 57.9%. 93 cases who saw the  doctor at the first time were divided into positive and negative treatment populations with SAT diagnostic technique,to these people,the agreement rate of the nucleic acid test results and SAT test results was 63.4%.Conclusion As a reference of laboratory-assisted diagnosis and treatment,nucleic acid isothermal amplification- immunochromatographic test has higher clinical application value for acute early brucellosis cases,but for differential diagnosis has no reference value for  brucellosis patients and non-brucellosis patients.
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Key words: nucleic acid, isothermal amplification, immunochromatography, brucellosis
nucleic acid, isothermal amplification, immunochromatography, brucellosis
nucleic acid, isothermal amplification, immunochromatography, brucellosis