吉林大学学报(医学版)

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HMGA2和E-cadherin在膀胱移行细胞癌组织中的表达及其意义

目的:探讨HMGA2和E-cadherin在膀胱移行细胞癌组织中的表达及其与临床分期、病理分级及预后的关系。方法:选取健康人正常膀胱组织5例、膀胱移行细胞癌组织49例。49例膀胱移行细胞癌组织分为非肌层浸润组(41例)、肌层浸润组(8例);低级别组(24例)、高级别组(25例)。应用免疫组织化学方法检测正常膀胱组织和膀胱移行细胞癌组织中HMGA2和E-cadherin蛋白的表达情况,并结合临床资料进行分析。结果:5例正常膀胱组织中HMGA2呈阴性表达、E-cadherin呈阳性表达;在49例膀胱癌组织中HMGA2阳性表达率为41%、E-cadherin的阴性表达率为43%;其中在非肌层浸润组(Tis~T1)、肌层浸润组(T2)膀胱癌组织中HMGA2的阳性表达率分别为34%和75%,HMGA2在肌层浸润组阳性表达率显著高于非肌层浸润组 (P<0.05);E-cadherin阴性表达率分别为37%和75%,E-cadherin在肌层浸润组阴性表达率显著高于非肌层浸润组 (P<0.05);在低级别、高级别肿瘤中HMGA2的阳性表达率分别为25%和56%,HMGA2在高级别组阳性表达率显著高于低级别组 (P<0.05),E-cadherin阴性表达率分别为25%和60%,E-cadherin在高级别组阴性表达率显著高于低级别组 (P<0.05);术后随访18~40个月,随访42例膀胱癌患者中复发18例。在复发组与未复发组中HMGA2的阳性表达率分别为56%和25%,HMGA2在复发组阳性表达率显著高于非复发组 (P<0.05);E-cadherin阴性表达率分别为67%和33%,E-cadherin在复发组阴性表达率显著高于非复发组 (P<0.05)。结论:HMGA2、E-cadherin的异常表达与膀胱移行细胞癌的恶性程度有关联,可以作为检测膀胱移行细胞癌临床分期、病理分级及预后的重要指标。   

  1. 吉林大学第一医院泌尿外科,吉林,长春 130021
  • 收稿日期:2013-04-25 出版日期:2013-09-28 发布日期:2013-12-13
  • 通讯作者: 李春萍(Tel:0431-81875804,E-mail:jfm63@sina.com) E-mail:jfm63@sina.com
  • 作者简介:姜凤鸣(1963-),男,吉林省长春市人,副教授,医学硕士,主要从事泌尿系肿瘤的诊治及基础研究。
  • 基金资助:

    吉林省科技厅科研基金资助课题(201115047)

Expressions and clinical significances of HMGA2 and E-cadherin in bladder transitional cell carcinoma tissue

膀胱肿瘤;高迁移率族蛋白A2;上皮钙依赖黏附蛋白E;免疫组织化学   

  1. Department of Urinary Surgery,First Hospital,Jilin University,Changchun 130021,China
  • Received:2013-04-25 Online:2013-09-28 Published:2013-12-13

摘要:

目的:探讨HMGA2和E-cadherin在膀胱移行细胞癌组织中的表达及其与临床分期、病理分级及预后的关系。方法:选取健康人正常膀胱组织5例、膀胱移行细胞癌组织49例。49例膀胱移行细胞癌组织分为非肌层浸润组(41例)、肌层浸润组(8例);低级别组(24例)、高级别组(25例)。应用免疫组织化学方法检测正常膀胱组织和膀胱移行细胞癌组织中HMGA2和E-cadherin蛋白的表达情况,并结合临床资料进行分析。结果:5例正常膀胱组织中HMGA2呈阴性表达、E-cadherin呈阳性表达;在49例膀胱癌组织中HMGA2阳性表达率为41%、E-cadherin的阴性表达率为43%;其中在非肌层浸润组(Tis~T1)、肌层浸润组(T2)膀胱癌组织中HMGA2的阳性表达率分别为34%和75%,HMGA2在肌层浸润组阳性表达率显著高于非肌层浸润组 (P<0.05);E-cadherin阴性表达率分别为37%和75%,E-cadherin在肌层浸润组阴性表达率显著高于非肌层浸润组 (P<0.05);在低级别、高级别肿瘤中HMGA2的阳性表达率分别为25%和56%,HMGA2在高级别组阳性表达率显著高于低级别组 (P<0.05),E-cadherin阴性表达率分别为25%和60%,E-cadherin在高级别组阴性表达率显著高于低级别组 (P<0.05);术后随访18~40个月,随访42例膀胱癌患者中复发18例。在复发组与未复发组中HMGA2的阳性表达率分别为56%和25%,HMGA2在复发组阳性表达率显著高于非复发组 (P<0.05);E-cadherin阴性表达率分别为67%和33%,E-cadherin在复发组阴性表达率显著高于非复发组 (P<0.05)。结论:HMGA2、E-cadherin的异常表达与膀胱移行细胞癌的恶性程度有关联,可以作为检测膀胱移行细胞癌临床分期、病理分级及预后的重要指标。

关键词:  , 膀胱肿瘤, 高迁移率族蛋白A2, 上皮钙依赖黏附蛋白E, 免疫组织化学

Abstract:

Abstract:Objective To investigate the expressions of HMGA2 and E-cadherin in bladder transitional cell carcinoma(BTCC) tissue and their relationship with clinical stage,pathological grade and prognosis.Methods The expressions of HMGA2 and E-cadherin in 49 cases of  BTCC (including 41 cases of non-muscle invasive bladder cancer,8 cases of muscle invasive bladder cancer;24 cases of low grade bladder cancer,25 cases of high  grade bladder cancer)and 5 specimens of normal bladder tissues were detected by  immunohistochemtry,and their correlations to the clinicopathologic features were analyzed.Results The HMGA2 expression was negative,but the E-cadherin expression was positive in 5 cases of  normal bladder tissue.The positive expression rate of HMGA2 and negative expression rate of E-cadherin in bladder cancer tissue was 41% and 43%,respectively;the positive expression rate of HMGA2 and negative expression rate of E-cadherin in non-muscle invasive bladder cancer and muscle invasive bladder cancer were 34%,75% and 37%,75%,respectively;the positive expression rate of HMGA2 in muscle invasive bladder cancer was apparently higher than that in  non-muscle invasive bladder cancer(P<0.05);the negative expression rate of E-cadherin in muscle invasive bladder cancer was  apparently higher than that in  non-muscle invasive bladder cancer(P<0.05).The positive expression rate of HMGA2 and negative expression rate of E-cadherin in low grade and high grade of BTCC were 25%,56% and 25%,60%, respectively;the positive expression rate of HMGA2 in high grade of BTCC was apparently higher than that in  low grade(P<0.05);the negative expression rate of E-cadherin in high grade of BTCC was apparently higher than that in  low grade of BTCC(P<0.05).The correlation between HMGA2 and E-cadherin was statistically significant (P<0.05).42 patients were followed up for 18-40 months,among which,18 reccured.The positive expression rate of HMGA2 and negative expression rate of E-cadherin in recurrence and non-recurrence groups were 56%,25% and 67%,33%,respectively;the positive expression rate of HMGA2 in recurrence group was apparently higher than that in  non-recurrence group(P<0.05);the negative expression rate of E-cadherin in recurrence group was apparently higher than that in non-recurrence group(P<0.05).Conclusion The abnormal expressions of HMGA2 and E-cadherin are related to the malignant tendency of BTCC,and they may be used as markers to predict clinical stage,pathological grade and prognosis of BTCC.

Key words: uring bladder neoplasms, high mobility group protein A2, E-cadherin, immunohistochemistry

中图分类号: 

  • R737.14