吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

人结肠癌细胞中血管生成拟态与肿瘤细胞迁移和侵袭能力的关系

刘见荣1,管宇2,可飞3,李文1,罗梅宏2,侯风刚1   

  • 收稿日期:2013-05-16 发布日期:2013-12-12
  • 通讯作者: 侯风刚(Tel:021-56639828-2602,E-mail:fghou555@hotmail.com) E-mail:fghou555@hotmail.com
  • 作者简介:刘见荣(1986-),女,河南省郑州市人,在读医学硕士,主要从事大肠癌中医基础与临床研究。
  • 基金资助:

    国家自然科学基金资助课题(81173221);上海教委创新项目资助课题(12YZ057)

Relationship  between vasculogenic mimicry in colon cancer cells and   migration and invasion of carcinoma cells

LIU Jian-rong1,GUAN Yu2,KE Fei3,LI Wen1,LUO Mei-hong2,HOU Feng-gang1   

  1. 1.Department of Oncology,Traditional Chinese Medical Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China;2. Laboratory Center,Traditional Chinese Medical Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China;3. Department of Pathology and Pathophysiology,School of Medical Sciences,Southeast University,Nanjing 210009,China
  • Received:2013-05-16 Published:2013-12-12

摘要:

目的:观察人结肠癌细胞中血管生成拟态(VM)的形成及密度,阐明VM与细胞的迁移、侵袭能力之间的相关性,为结肠癌的临床治疗提供新的切入点和理论依据。方法:体外三维培养人结肠癌细胞株HCT8、HCT116、LS174T和HT29,在倒置显微镜下观察VM形成情况,计算VM密度;采用划痕实验观察4株细胞的迁移能力;Transwell小室法观察4株细胞的侵袭能力;直线相关分析法分析VM密度与肿瘤细胞迁移距离和穿膜细胞数之间的相关性。结果:HCT8和HCT116细胞在体外三维培养条件下均能够形成VM,其密度分别为(6.75±0.957)和(5.75±0.957)个/视野;LS174T和HT29细胞则不能形成VM。划痕实验,HCT8、HCT116和LS174T细胞在图片上的迁移距离分别为(3.833±0.831)、(3.967±0.975)和(0.817±0.333)cm,HT29细胞无迁移趋势。Transwell小室法,HCT8、HCT116、LS174T和HT29细胞在高倍视野下的穿膜细胞数分别为(71.6±4.506)、(22.4±1.517)、(0.6±0.894)和(0.2±0.447)个。VM密度与迁移距离呈正相关关系(r=0.934,P<0.05),VM密度和Transwell侵袭实验中的穿膜细胞数亦呈正相关关系(r=0.853,P<0.05)。结论:人结肠癌细胞中存在VM现象,VM可能与结肠癌的迁移、侵袭能力有关联。

关键词: 结肠肿瘤, 血管生成拟态, 三维培养, 迁移, 侵袭

Abstract:

Abstract:Objective
To investigate  the formation and density of vasculogenic minicry(VM)  in colon cancer cells,and   to clarify the relevance between VM and cell migration and invasion,and to provide a new starting point and theoretical basis for clinical treatment of colon carcinoma.Methods The colon cancer cell lines HCT8,HCT116,LS174T and HT29 were cultured in three-dimensional condition in vitro,and the formation of VM was observed under inverted microscope;then the density of VM was calculated;the cell migration was tested by wound healing assay,while the cell invasion was observed by Transwell  assay;the linear correlation analysis was used to detect  the relationship between the  density of VM and tumor cell migration distance or the number of cells penetrating the membrane.Results The   HCT8 and HCT116 cells cultured in three-dimensional condition could form VM  in vitro,and the densities of VM of HCT8 cells were (6.75±0.957) and (5.75±0.957)/ visual field,while the LS174T,HT29 cells were unable to form VM.The results of wound healing assay showed that the migration distances on the picture   of HCT8,HCT116 and LS174T cells were (3.833±0.831),(3.967±0.975), and (0.817±0.333) cm,respectively;but the  HT29 cells had no migration trend.The Transwell assay results showed that the number of HCT8,HCT116,LS174T, and HT29 cells penetrating the membrane of every vision was 71.6±4.506,22.4±1.517,0.6±0.894,and 0.2±0.447,respectively.The density of VM was positively correlated with the migration distance (r=0.934,P<0.05) and the number of cells penetrating the membrane detected by Transwell  assay (r=0.853,P<0.05).Conclusion VM exists in the colon cancer cells,and VM may be contributed to the migration and invasion of colon cancer.

Key words:  colon neoplasms, vasculogenic mimicry, three-dimensional culture, migration, invasion

中图分类号: 

  • R735.3