热休克蛋白融合蛋白对人树突状细胞分化成熟的刺激作用

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• 基础研究 • 下一篇

热休克蛋白融合蛋白对人树突状细胞分化成熟的刺激作用

万敏¹, 张培因¹, 王宣军², 吴秀丽¹, 卫红飞¹, 王燕媚¹, 于永利², 王丽颖^1*，邓平³，张慧杰³

1.吉林大学基础医学院分子生物学教研室，吉林　长春130021；2. 吉林大学基础医学院免疫学教研室，吉林长春130021；3.吉林省长春市中心血站，吉林长春130033

收稿日期:2004-02-27 修回日期:1900-01-01 出版日期:2004-11-28 发布日期:2004-11-28
通讯作者: 王丽颖

Stimulation of HSP-fusion protein on differentiationand maturation of human dendritic cells

WAN Min¹, ZHANG Pei-yin¹, WANG Xuan-jun², WU Xiu-li¹, WEI Hong-fei¹, WANG Yan-mei¹, YU Yong-li², WANG Li-ying^1*，DENG Ping³, ZHANG Hui-jie³

1. Department of Molecular Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China;2. Deparment of Immunology, School of Basic Medical Sciences, Jilin University, Changchun130021,China；3.Changchun Blood Center, Jilin Province, Changchun 130033,China

Received:2004-02-27 Revised:1900-01-01 Online:2004-11-28 Published:2004-11-28
Contact: WANG Li-ying

摘要/Abstract

摘要： 目的：研究热休克蛋白融合蛋白对人树突状细胞分化成熟的刺激作用。方法：采用GM-CSF和IL-4将人外周血单核细胞诱导成未成熟树突状细胞，然后用热休克蛋白融合蛋白刺激其分化成熟，以单核细胞条件培养液（MCM）和模拟的单核细胞条件培养液（MCM mimic）为对照。以流式细胞仪检测树突状细胞表面分子的表达情况。结果：热休克蛋白融合蛋白可以刺激树突状细胞表面表达CD40、CD80、CD86和HLA-A2分子。结论：热休克蛋白融合蛋白可诱导人树突状细胞分化成熟。

关键词: 热休克蛋白质类, 重组融合蛋白质类, 生物合成

Abstract: Objective To study the effects of heat-shock protein (HSP) fusion protein on the differentiation and maturation of human monocyte derived dendritic cells (DC). Methods Human peripheral blood mononuclear cells (PBMC) were induced into immature dendritic cells (iDC) in the presences of GM-CSF and IL-4. On the 5th day the iDC was stimulated with HSP-fusion protein. Monocyte conditioned medium (MCM) and MCM mimic, a cocktail of IL-1β，IL-6，TNF-α and PGE2 were used as control. On the 7th day the DC stimulated with the HSP-fusion protein was stained with FITC-labeled antibodies against CD40, CD80, CD86, and HLA-A2 separately and the results were determined by FACS. Results HSP-fusion protein could up-regulate the iDC to express CD40, CD80, CD86, and HLA-A2 molecules. Conclusion HSP-fusion protein can induce the differentiation and maturation of human DC.

Key words: heat-shock proteins, recombinant fusion proteins, biosynthsis

中图分类号:

R392.12

万敏, 张培因, 王宣军, 吴秀丽, 卫红飞, 王燕媚, 于永利, 王丽颖，邓平，张慧杰. 热休克蛋白融合蛋白对人树突状细胞分化成熟的刺激作用[J]. J4, 2004, 30(6): 835-838.

WAN Min, ZHANG Pei-yin, WANG Xuan-jun, WU Xiu-li, WEI Hong-fei, WANG Yan-mei, YU Yong-li, WANG Li-ying，DENG Ping, ZHANG Hui-jie. Stimulation of HSP-fusion protein on differentiationand maturation of human dendritic cells[J]. J4, 2004, 30(6): 835-838.

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热休克蛋白融合蛋白对人树突状细胞分化成熟的刺激作用

Stimulation of HSP-fusion protein on differentiationand maturation of human dendritic cells

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