含MT蛋奶粉对小鼠辐射损伤的保护作用

doi:吉林省科技厅

含MT蛋奶粉对小鼠辐射损伤的保护作用

赵红光¹，王志成¹，杜翔¹，吕吉吉¹，姜晓艳¹，史经宇²，龚守良^1*

1. 吉林大学公共卫生学院卫生部放射生物学重点实验室，吉林长春130021;2. 哈尔滨春源生物科技开发有限公司，黑龙江哈尔滨150036

收稿日期:2004-11-16 修回日期:1900-01-01 出版日期:2005-07-28 发布日期:2005-07-28
通讯作者: 龚守良

Protective effects of egg-milk with MTon mice damaged by irradiation

ZHAO Hong-guang¹, WANG Zhi-cheng¹, DU Xiang¹, LU-Zhe¹,JIANG Xiao-yan¹,SHI Jing-yu², GONG Shou-liang^1*

1. MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China；2. Haerbin Chunyuan Biotechnology Development Co.,LTD, Haerbin 150036,China

Received:2004-11-16 Revised:1900-01-01 Online:2005-07-28 Published:2005-07-28
Contact: GONG Shou-liang

摘要/Abstract

摘要： 目的：研究含金属硫蛋白（MT）蛋奶粉对小鼠电离辐射损伤的保护作用。方法：小鼠受2.5 Gy （12.5 mGy·min^-1）X射线照射后，灌胃给予含 MT蛋奶粉，连续14 d后检测外周血白细胞数、淋巴细胞增殖率、股骨骨髓细胞DNA含量、胸腺细胞周期进程以及肝脏和肾脏丙二醛（MDA）含量和抗氧化酶（SOD、CAT和GSH-Px）活性。结果：受照射小鼠给予含MT蛋奶粉后，尤其是含中高剂量MT蛋奶粉组，白细胞数、淋巴细胞增殖率和股骨骨髓细胞DNA含量明显高于辐射对照组（P<0.01）；含MT蛋奶粉加照射组胸腺细胞G0/G₁期细胞数明显低于辐射对照组（P<0.01），S期和G₂/M期细胞数明显高于辐射对照组（P<0.01或P<0.05）；照射组小鼠肝脏和肾脏MDA含量较正常对照组显著增加（P<0.01或P<0.05），抗氧化酶SOD、CAT和GSH-Px活性则较正常对照组显著降低（P<0.01或P<0.05），而含MT蛋奶粉加照射组与辐射对照组比较，肝脏和肾脏MDA水平明显降低（P<0.01），抗氧化酶SOD、CAT和GSH-Px活性显著增高（P<0.01或P<0.05）。结论：含MT蛋奶粉可明显改善X射线照射对小鼠的损伤效应，具有保护作用。［关键词］［中图分类号］

关键词: 药理学, 辐射损伤, 实验性, 丙二醛, DNA

Abstract: Objective To explore the protective effects of egg-milk with metallothionein (MT) on mice damaged by irradiation. Methods After the mice were irradiated with 2.5 Gy (12.5 mGy·min^-1) X-rays, they were fed with egg-milk with MT for 14 days. The level of WBC in peripheral blood, lymphocyte proliferation rate, DNA content in marrow cells, malondiadehyde (MDA) levels and the activities of antioxidant enzymes (SOD, CAT and GSH-Px) in liver and kidney were measured. Results After the irradiated mice were fed with egg-milk with MT, especially in the egg-milk with MT of mild and high doses plus irradiation groups, the WBC number, lymphocyte proliferation rate and DNA content of marrow cells in the mice were significantly higher than those in the irradiation group (P<0.01), at the same time, the percentage of thymocyte G0/G₁ phase of these mice were significantly lower than that of the irradiation group (P<0.01), and the percentage of thymocyte S and G₂/M phase were signifi cantly higher than tho se in the irradiation group　(P<0.01 or P<0.05). Compared with the no rmal control, MDA levels in liver and kidney of the irradiation group increased significantly (P<0.01 or P<0.05), and the antioxidant enzyme activit ies of SOD, CAT and GSH-Px decreased significantly (P<0.01 or P<0.05). While in the egg-milk with MT plus irradiation groups, the MDA contents were significantly lower than those in the irradiation group (P<0.01) and the activities of SOD, CAT and GSH-Px were significantly higher than those in the irra diation group (P<0.01 or P<0.05). Conclusion The egg-milk with MT has protective effects on mice injuried by irradiation. Key words：metallothionein/pharmacology; radiation injuries, e xperimental;malondialdehyde; DNA

Key words: pharmacology, radiation injuries, experimental, malondialdehyde, DNA

中图分类号:

R818.74

赵红光，王志成，杜翔，吕吉吉，姜晓艳，史经宇，龚守良. 含MT蛋奶粉对小鼠辐射损伤的保护作用[J]. J4, 2005, 31(4): 543-546.

ZHAO Hong-guang, WANG Zhi-cheng, DU Xiang, LU-Zhe,JIANG Xiao-yan,SHI Jing-yu, GONG Shou-liang. Protective effects of egg-milk with MTon mice damaged by irradiation[J]. J4, 2005, 31(4): 543-546.

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