“温阳补气”针法对实验性自身免疫性重症肌无力大鼠神经肌肉接头处AchR mRNA表达的影响

doi:10.13481/j.1671-587x.20160506

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (05): 872-876.doi: 10.13481/j.1671-587x.20160506

“温阳补气”针法对实验性自身免疫性重症肌无力大鼠神经肌肉接头处AchR mRNA表达的影响

胡英华¹, 王婷婷², 王喜臣³, 王洪峰³

1. 长春中医药大学针推学院针灸基础教研室, 吉林长春 130117;
2. 北京市第一中西医结合医院针灸科, 北京 100026;
3. 长春中医药大学针灸基础重点研究室, 吉林长春 130117

收稿日期:2016-01-30 出版日期:2016-09-28 发布日期:2016-09-29
通讯作者: 王洪峰,教授,博士研究生导师(Tel:0431-86172415,E-mail:24974606@qq.com) E-mail:24974606@qq.com
作者简介:胡英华(1974-),女,吉林省永吉县人,医学博士,讲师,主要从事特定穴配伍规律理论和临床方面的研究。
基金资助:
国家自然科学基金资助课题（81173344）；教育部博士点科研基金资助课题（20112227120006）；吉林省教育厅重点项目资助课题[吉教科合字（2012第64号）]

Effect of “Warming Yang and Nourishing Qi” Acupuncture on AChR mRNA expression in neuromuscular junction of rats with experimental autoimmune myasthenis gravis

HU Yinghua¹, WANG Tingting², WANG Xichen³, WANG Hongfeng³

1. Acupuncture-Moxibustion Basic Teaching and Research Section, College of Acupuncture-Moxibustion and Massage, Changchun University of Chinese Medicine, Changchun 130117, China;
2. Department of Acupuncture and Moxibustion, Beijing First Hospital of Integrated Chinese and Western Medicine, Beijing 100026, China;
3. Key Research Center of Acupuncture and Moxibustion, Changchun University of Chinese Medicine, Changchun 130117, China

Received:2016-01-30 Online:2016-09-28 Published:2016-09-29

摘要/Abstract

摘要：

目的：探讨“温阳补气”针法对实验性自身免疫性重症肌无力（EAMG）大鼠神经肌肉接头处（NMJ）乙酰胆碱受体（AchR）mRNA表达的影响，阐明“温阳补气”针法对治疗EAMG的效应机制。方法：AchRα1 129-145多肽片段主动免疫60只SPF三级Lewis雌性大鼠，构建EAMG大鼠模型，模型制备成功后，随机选取30只大鼠分为模型对照组、针刺治疗组和药物对照组，每组10只，并将同批购进且相同条件下饲养的另外10只SPF三级Lewis大鼠设为空白对照组。空白对照组及模型对照组大鼠不予任何干预，针灸治疗组大鼠施以“温阳补气”针法治疗，药物对照组大鼠施以溴吡斯的明灌胃治疗。15d后，取实验大鼠腓肠肌NMJ处周围组织，采用RT-PCR法检测AchR mRNA表达水平，并定量分析各组之间的表达差异。结果：与空白对照组比较，针刺治疗组、药物对照组和模型对照组大鼠NMJ处γ-AchR和ε-AchR mRNA相对表达水平均升高（P<0.01）；与模型对照组比较，针灸治疗组与药物对照组大鼠NMJ处γ-AchR和ε-AchR mRNA相对表达水平均升高（P<0.01）。结论：“温阳补气”针法能够有效提高EAMG大鼠AchR mRNA表达水平，提示该方法可能是治疗重症肌无力的有效方法。

关键词: ", 温阳补气", 针法, 乙酰胆碱受体, 实验性自身免疫性重症肌无力

Abstract:

Objective: To observe the effect of "Warming Yang and Nourishing Qi" Acupuncture on the acetylcholine receptor (AchR) mRNA expression in neuromuscular junction (NMJ) of the rats with experimental autoimmune myasthenis gravis (EAMG),and to analyze the mechanism of acupuncture treatment for EAMG. Methods: AchRα1 129-145 peptide fragments were used to immune the 60 female Lewis rats to establish the EAMG rat models. 30 EAMG rats were selected randomly and divided into model control group, acupuncture treatment group and drug control group(n=10). Another 10 SPF- "grade three" female Lewis rats, which were raised together with EAMG rats, were used as blank control group. The rats in model control group and blank control group had no treatment. The rats in acupuncture treatment group were treated with "Warming Yang and Nourishing Qi" Acupuncture.The rats in drug control group were treated with pyridostigmine bromide by gavage. The tissue samples in NMJ of gastrocnemius of the rats were collected, and the expression levels of AChR mRNA were detected with Real-time PCR method; the differences among various groups were analyzed quantitatively. Results: Compared with blank control group,the expression levels of γ-AChR mRNA and ε-AChR mRNA in NMJ of the rats in acupuncture treatment group drug control group and model control group were significantly increased (P<0.01). Compared with model control group,the γ- AChR mRNA and ε- AChR mRNA in NMJ of the rats in drug acupuncture treatment group and control group were significantly increased (P<0.01). Conclusion: "Warming Yang and Nourishing Qi" Acupuncture can effectively improve the expression level of AchR mRNA and it is an effective treatment method for myasthenia gravis.

Key words: "Warming Yang and Nourishing Qi", Acupuncture, acetylcholine receptor, experimental autoimmune myasthenia gravis

中图分类号:

R245

胡英华, 王婷婷, 王喜臣, 王洪峰. “温阳补气”针法对实验性自身免疫性重症肌无力大鼠神经肌肉接头处AchR mRNA表达的影响[J]. 吉林大学学报(医学版), 2016, 42(05): 872-876.

HU Yinghua, WANG Tingting, WANG Xichen, WANG Hongfeng. Effect of “Warming Yang and Nourishing Qi” Acupuncture on AChR mRNA expression in neuromuscular junction of rats with experimental autoimmune myasthenis gravis[J]. Journal of Jilin University Medicine Edition, 2016, 42(05): 872-876.

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“温阳补气”针法对实验性自身免疫性重症肌无力大鼠神经肌肉接头处AchR mRNA表达的影响

Effect of “Warming Yang and Nourishing Qi” Acupuncture on AChR mRNA expression in neuromuscular junction of rats with experimental autoimmune myasthenis gravis

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